LOCUS       BBa_K812031              726 bp    DNA     linear       23-Dec-2024
DEFINITION  sfGFP with kozak sequence for eukaryotic expression
ACCESSION   BBa_K812031
VERSION     BBa_K812031.1  
FEATURES             Location/Qualifiers
     RBS             1..6
                     /label=kozak
     CDS             7..720
                     /label=sfGFP
ORIGIN
        1 gccaccatgc gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg
       61 gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact
      121 aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt accttggccg
      181 actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg
      241 aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt
      301 tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc
      361 ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa tatcctgggc
      421 cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga taaacaaaaa
      481 aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag cgtgcagctg
      541 gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat
      601 cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg
      661 gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa
      721 taataa
//
LOCUS       BBa_K812331              726 bp    DNA     linear       23-Dec-2024
DEFINITION  sfGFP in pCS2+ with HSP70 promoter
ACCESSION   BBa_K812331
VERSION     BBa_K812331.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=Kozak
     CDS             7..720
                     /label=sfGFP
     misc_feature    721..726
                     /label=stop
ORIGIN
        1 gccaccatgc gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg
       61 gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact
      121 aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt accttggccg
      181 actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg
      241 aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt
      301 tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc
      361 ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa tatcctgggc
      421 cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga taaacaaaaa
      481 aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag cgtgcagctg
      541 gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat
      601 cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg
      661 gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa
      721 taataa
//