BBa_K812031 1 BBa_K812031 sfGFP with kozak sequence for eukaryotic expression 2012-09-19T11:00:00Z 2015-05-08T01:13:27Z PCR amplified with kozak sequence from DNA coming from Alfonso Jaramillo's lab Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts. false true _1069_ 0 12937 9 In stock false PCR and cloned in pSB1C3 using EcoRI and PstI false Chauris Karine annotation2187642 1 sfGFP range2187642 1 7 720 annotation2187641 1 kozak range2187641 1 1 6 BBa_K812331 1 sfGFP sfGFP in pCS2+ with HSP70 promoter 2012-09-19T11:00:00Z 2015-05-08T01:13:28Z pCS2+ is from K812000. sfGFP is from Alphonso Jamarillo's lab, the Biobrick is K812031. HSP70 is from the article: Love, N.R. et al., 2011. pTransgenesis: a cross-species, modular transgenesis resource. Development (Cambridge, England), 138(24), pp.5451???8 The sfGFP protein is expressed under the inducible promoter Heat Shock Promoter 70 (HSP70). This part is complete and can be directly injected into fertilized frog's egg. To induce the expression the tadpole must be heat (for example from 21??C to 34??C). true false _1069_ 0 12000 9 Discontinued false pCS2+ is from K812000 and the promoter CMV was replaced by HSP70 with double digestion: SalI and HindIII digestion. HSP 70 is amplified with primers having the prefix SalI and the suffix HindIII. false Tristan Cerisy annotation2188297 1 sfGFP range2188297 1 7 720 annotation2188298 1 stop range2188298 1 721 726 annotation2188296 1 Kozak range2188296 1 1 6 BBa_K812331_sequence 1 gccaccatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa BBa_K812031_sequence 1 gccaccatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z