BBa_K812031 1 BBa_K812031 sfGFP with kozak sequence for eukaryotic expression 2012-09-19T11:00:00Z 2015-05-08T01:13:27Z PCR amplified with kozak sequence from DNA coming from Alfonso Jaramillo's lab Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts. false true _1069_ 0 12937 9 In stock false PCR and cloned in pSB1C3 using EcoRI and PstI false Chauris Karine annotation2187641 1 kozak range2187641 1 1 6 annotation2187642 1 sfGFP range2187642 1 7 720 BBa_K812333 1 BBa_K812333 pCS2+ with HSP70 promoter and sfGFP reporter 2012-09-19T11:00:00Z 2015-05-08T01:13:28Z sfGFP is from the Alphonso Jamarillo's lab. HSP70 is from the article: Love, N.R. et al., 2011. pTransgenesis: a cross-species, modular transgenesis resource. Development (Cambridge, England), 138(24), pp.5451???8 pCS2+ is from the part K812000, the promoter was changed by HSP70 and J04450 by sfGFP (K812031) which contain the kozak sequence. The plasmid is ready to use in fertilized frog's egg. The expression of sfGFP occur after an induction by heat shock (for example from 24??C to 34??C). true false _1069_ 0 12000 9 Discontinued false The promoter CMV from the part K812000 was changed by HSP70 with restriction sites SalI and HindIII. The reporter is sfGFP from K812031, it was integrate using Biobrik prefix EcoRI and suffix PstI present in our pCS2+ (Biobricked)K812000. false Tristan Cerisy annotation2188254 1 Kozak range2188254 1 1 6 annotation2188256 1 sfGFP range2188256 1 7 720 annotation2188255 1 stop range2188255 1 721 726 BBa_K812333_sequence 1 gccaccatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa BBa_K812031_sequence 1 gccaccatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z