BBa_K814002 1 BBa_K814002 dehydroquinate O-methyltrasferase (O-MT) generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1) Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. Dehydroquinate O-methyltransferase (O-MT) catalyzes the second step in this pathway, converting dehydroquinate to 4-deoxygadusol. This part includes a modified constitutive lac promoter (lacPmod), and RBS and the open reading frame of O-MT. This part can be used to express O-MT in E. coli. false false _1071_ 0 9070 9 Not in stock false Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites. false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn annotation2210145 1 lacP' range2210145 1 1 127 annotation2210146 1 RBS range2210146 1 128 133 annotation2210147 1 O-MT range2210147 1 140 980 BBa_K814002_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgacaaatgtgattgtccaaccaacagctagacctgttacaccattgggaattttaaccaagcagttagaagccatagtccaagaggttaagcaacatccagatttacctggggaattgatagcaaacatccatcaggcttggcgtttagccgcaggtatagacccttatttggaagaatgcaccactccagaatctcctgaactcgctgcattggcaaaaaccacagccaccgaagcctggggagaacacttccacggaggtacaaccgtccgtcctctagaacaagagatgctttctggtcatatcgaaggacaaaccttaaagatgtttgttcacatgaccaaagctaaaaaagtcttagaaattgggatgtttaccggttattcggcgctggcgatggcggaagcattaccagaggatggactgcttgtggcttgtgaagttgacccttacgcggcggaaattggacagaaagcctttcaacaatctccccacggtggaaagattcgtgtggaattggatgcagccttagcaactcttgataagttagcagaagctggggagtcttttgacttggtatttatcgacgcagataaaaaagagtatgtagcctattttcacaagttgctaggtagcagtttgttagcaccagatggctttatttgtgtagataacaccttattacaaggggaagtttatctaccagcagaggaacgtagcgtcaatggtgaagcgatcgcgcaatttaatcatacagtagctatagacccccgtgtagaacaggttttgttgccgttgcgagatggtttaacaattatccgcagaatacaaccttaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z