LOCUS       BBa_K814003             2880 bp    DNA     linear       22-Nov-2024
DEFINITION  shinorine non-ribosomal peptide synthase (NRPS) generator
ACCESSION   BBa_K814003
VERSION     BBa_K814003.1  
FEATURES             Location/Qualifiers
     promoter        1..127
                     /label=lacP'
     RBS             128..133
                     /label=RBS
     CDS             140..2806
                     /label=NRPS
ORIGIN
        1 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
       61 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgtc
      121 tagtagaagg aggagatcta tgcagactat agattttaat attcgtaagt tacttgtaga
      181 gtggaacgcg acccacagag attatgatct ttcccagagt ttacatgaac taattgtagc
      241 tcaagtagaa cgaacacctg aggcgatcgc tgtcaccttt gacaagcaac aactaactta
      301 tcaagaacta aatcataaag caaaccagct aggacattat ttacaaacat taggagtcca
      361 gccagaaacc ctggtaggcg tttgtttaga acgttcctta gaaatggtta tctgtctttt
      421 aggaatcctc aaagctgggg gtgcttatgt tcctattgac cctgaatatc ctcaagaacg
      481 catagcttat atgctagaag attctcaggt gaaggtacta ctaactcaag aaaaattact
      541 caatcaaatt ccccaccatc aagcacaaac tatctgtgta gatagggaat gggagaaaat
      601 ttccacacaa gctaatacca atcccaaaag taatataaaa acggataatc ttgcttatgt
      661 aatttacacc tctggttcca ctggtaaacc aaaaggtgca atgaacaccc acaaaggtat
      721 ctgtaatcgc ttattgtgga tgcaggaagc ttatcaaatc gattccacag atagcatttt
      781 acaaaaaacc ccctttagtt ttgatgtttc cgtttgggag ttcttttgga ctttattaac
      841 tggcgcacgt ttggtaatag ccaaaccagg cggacataaa gatagtgctt acctcatcga
      901 tttaattact caagaacaaa tcactacgtt gcattttgtc ccctcaatgc tgcaagtgtt
      961 tttacaaaat cgccatgtaa gcaaatgcag ctctctaaaa agagttattt gtagcggtga
     1021 agctttatct atagatttac aaaatagatt tttccagcat ttgcaatgtg aattacataa
     1081 cctctatggc ccgacagaag cagcaattga tgtcacattt tggcaatgta gaaaagatag
     1141 taatttaaag agtgtaccta ttggtcgtcc cattgctaat actcaaattt atattcttga
     1201 tgccgattta caaccagtaa atattggtgt cactggtgaa atttatattg gtggtgtagg
     1261 ggttgctcgt ggttatttga ataaagaaga attgaccaaa gaaaaattta ttattaatcc
     1321 ctttcccaat tctgagttta agcgacttta taaaacaggt gatttagctc gttatttacc
     1381 cgatggaaat attgaatatc ttggtagaac agattatcaa gtaaaaattc ggggttatag
     1441 aattgaaatt ggcgagattg aaaatgtttt atcttcacac ccacaagtca gagaagctgt
     1501 agtcatagcg cgggatgata acgctcaaga aaaacaaatc atcgcttata ttacctataa
     1561 ctccatcaaa cctcagcttg ataatctgcg tgatttccta aaagcaaggc tacctgattt
     1621 tatgattcca gccgcttttg tgatgctgga gcatcttcct ttaactccca gtggtaaagt
     1681 agaccgtaag gcattaccta agcctgattt atttaattat agtgaacata attcctatgt
     1741 agcgcctcgg aatgaagttg aagaaaaatt agtacaaatc tggtcgaata ttctgcattt
     1801 acctaaagta ggtgtgacag aaaacttttt cgctattggt ggtaattccc tcaaagctct
     1861 acatttaatt tctcaaattg aagagttatt tgctaaagag atatccttag caacactttt
     1921 aacaaatcca gtaattgcag atttagccaa ggttattcaa gcaaacaacc aaatccataa
     1981 ttcaccccta gttccaattc aaccacaagg taagcagcag cctttctttt gtatacatcc
     2041 tgctggtggt catgttttat gctattttaa actcgcacaa tatataggaa ctgaccaacc
     2101 attttatggc ttacaagctc aaggatttta tggagatgaa gcacccttga cgcgagttga
     2161 agatatggct agtctctacg tcaaaactat tagagaattt caaccccaag ggccttatcg
     2221 tgtcgggggg tggtcatttg gtggagtcgt agcttatgaa gtagcacagc agttacatag
     2281 acaaggacaa gaagtatctt tactagcaat attagattct tacgtaccga ttctgctgga
     2341 taaacaaaaa cccattgatg acgtttattt agttggtgtt ctctccagag tttttggcgg
     2401 tatgtttggt caagataatc tagtcacacc tgaagaaata gaaaatttaa ctgtagaaga
     2461 aaaaattaat tacatcattg ataaagcacg gagcgctaga atattcccgc ctggtgtaga
     2521 acgtcaaaat aatcgccgta ttcttgatgt tttggtggga actttaaaag caacttattc
     2581 ctatataaga caaccatatc caggaaaagt cactgtattt cgagccaggg aaaaacatat
     2641 tatggctcct gacccgacct tagtttgggt agaattattt tctgtaatgg cggctcaaga
     2701 aattaagatt attgatgtcc ctggaaacca ttattcgttt gttctagaac cccatgtaca
     2761 ggttttagca cagcgtttac aagattgtct ggaaaataat tcataagcgg ccgcctcgag
     2821 catcaccatc accatcactg attctcctta cgcatctgtg cggtatttca caccgcatgt
//