BBa_K814003 1 BBa_K814003 shinorine non-ribosomal peptide synthase (NRPS) generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1) Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. NRPS catalyzes the final step in the pathway, converting mycosporine-glycine into shinorine. This part includes a modified constitutive lac promoter (lacP???), an RBS and the open reading frame of NRPS. This part can be used to express the shinorine NRPS in E. coli. false false _1071_ 0 9070 9 It's complicated false Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites. false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn annotation2210150 1 NRPS range2210150 1 140 2806 annotation2210149 1 RBS range2210149 1 128 133 annotation2210148 1 lacP' range2210148 1 1 127 BBa_K814003_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgcagactatagattttaatattcgtaagttacttgtagagtggaacgcgacccacagagattatgatctttcccagagtttacatgaactaattgtagctcaagtagaacgaacacctgaggcgatcgctgtcacctttgacaagcaacaactaacttatcaagaactaaatcataaagcaaaccagctaggacattatttacaaacattaggagtccagccagaaaccctggtaggcgtttgtttagaacgttccttagaaatggttatctgtcttttaggaatcctcaaagctgggggtgcttatgttcctattgaccctgaatatcctcaagaacgcatagcttatatgctagaagattctcaggtgaaggtactactaactcaagaaaaattactcaatcaaattccccaccatcaagcacaaactatctgtgtagatagggaatgggagaaaatttccacacaagctaataccaatcccaaaagtaatataaaaacggataatcttgcttatgtaatttacacctctggttccactggtaaaccaaaaggtgcaatgaacacccacaaaggtatctgtaatcgcttattgtggatgcaggaagcttatcaaatcgattccacagatagcattttacaaaaaaccccctttagttttgatgtttccgtttgggagttcttttggactttattaactggcgcacgtttggtaatagccaaaccaggcggacataaagatagtgcttacctcatcgatttaattactcaagaacaaatcactacgttgcattttgtcccctcaatgctgcaagtgtttttacaaaatcgccatgtaagcaaatgcagctctctaaaaagagttatttgtagcggtgaagctttatctatagatttacaaaatagatttttccagcatttgcaatgtgaattacataacctctatggcccgacagaagcagcaattgatgtcacattttggcaatgtagaaaagatagtaatttaaagagtgtacctattggtcgtcccattgctaatactcaaatttatattcttgatgccgatttacaaccagtaaatattggtgtcactggtgaaatttatattggtggtgtaggggttgctcgtggttatttgaataaagaagaattgaccaaagaaaaatttattattaatccctttcccaattctgagtttaagcgactttataaaacaggtgatttagctcgttatttacccgatggaaatattgaatatcttggtagaacagattatcaagtaaaaattcggggttatagaattgaaattggcgagattgaaaatgttttatcttcacacccacaagtcagagaagctgtagtcatagcgcgggatgataacgctcaagaaaaacaaatcatcgcttatattacctataactccatcaaacctcagcttgataatctgcgtgatttcctaaaagcaaggctacctgattttatgattccagccgcttttgtgatgctggagcatcttcctttaactcccagtggtaaagtagaccgtaaggcattacctaagcctgatttatttaattatagtgaacataattcctatgtagcgcctcggaatgaagttgaagaaaaattagtacaaatctggtcgaatattctgcatttacctaaagtaggtgtgacagaaaactttttcgctattggtggtaattccctcaaagctctacatttaatttctcaaattgaagagttatttgctaaagagatatccttagcaacacttttaacaaatccagtaattgcagatttagccaaggttattcaagcaaacaaccaaatccataattcacccctagttccaattcaaccacaaggtaagcagcagcctttcttttgtatacatcctgctggtggtcatgttttatgctattttaaactcgcacaatatataggaactgaccaaccattttatggcttacaagctcaaggattttatggagatgaagcacccttgacgcgagttgaagatatggctagtctctacgtcaaaactattagagaatttcaaccccaagggccttatcgtgtcggggggtggtcatttggtggagtcgtagcttatgaagtagcacagcagttacatagacaaggacaagaagtatctttactagcaatattagattcttacgtaccgattctgctggataaacaaaaacccattgatgacgtttatttagttggtgttctctccagagtttttggcggtatgtttggtcaagataatctagtcacacctgaagaaatagaaaatttaactgtagaagaaaaaattaattacatcattgataaagcacggagcgctagaatattcccgcctggtgtagaacgtcaaaataatcgccgtattcttgatgttttggtgggaactttaaaagcaacttattcctatataagacaaccatatccaggaaaagtcactgtatttcgagccagggaaaaacatattatggctcctgacccgaccttagtttgggtagaattattttctgtaatggcggctcaagaaattaagattattgatgtccctggaaaccattattcgtttgttctagaaccccatgtacaggttttagcacagcgtttacaagattgtctggaaaataattcataagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z