BBa_K814004 1 BBa_K814004 mycosporine-glycine biosynthetic pathway 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z All open reading frames in this composite part were taken from Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1). Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. Dehydroquinate-O-methyltransferase (O-MT) then generates 4-deoxygadusol, which is converted to mycosprine-glycine by ATP-grasp (ATPG). This composite part contains protein generators for DHQS, O-MT and ATPG. E. coli transformed with this composite part are able to synthesize mycosporine-glycine, as detected by HPLC analysis of the media. 4-deoxygadusol is also detected in HPLC analysis. false false _1071_ 0 9070 9 Not in stock false Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota)for A. variabilis. From here, PCR primer extension was used to clone each individual gene with flanking restriction digest sites to be used for vector cloning. Each gene was cloned into an individual pUCBB BioBrick??? cloning vectors containing the lacP' promoter and an RBS. The resulting generators were then stacked into a single BioBrick??? cloning vector using classic assembly techniques false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn component2210158 1 BBa_K814001 component2210162 1 BBa_K814002 component2210154 1 BBa_K814000 annotation2210158 1 BBa_K814001 range2210158 1 1454 3042 annotation2210154 1 BBa_K814000 range2210154 1 1 1445 annotation2210162 1 BBa_K814002 range2210162 1 3051 4102 BBa_K814001 1 BBa_K814001 ATP-grasp (ATPG) generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1) Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. ATP-grasp (ATPG) catalyzes the third step in this pathway, 4-deoxygadusol into mycosporine-glycine. This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli. false false _1071_ 0 9070 9 It's complicated false Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites. false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn annotation2210142 1 lacP' range2210142 1 1 127 annotation2210143 1 RBS range2210143 1 128 133 annotation2210144 1 ATPG range2210144 1 140 1517 BBa_K814002 1 BBa_K814002 dehydroquinate O-methyltrasferase (O-MT) generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1) Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. Dehydroquinate O-methyltransferase (O-MT) catalyzes the second step in this pathway, converting dehydroquinate to 4-deoxygadusol. This part includes a modified constitutive lac promoter (lacPmod), and RBS and the open reading frame of O-MT. This part can be used to express O-MT in E. coli. false false _1071_ 0 9070 9 Not in stock false Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites. false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn annotation2210145 1 lacP' range2210145 1 1 127 annotation2210147 1 O-MT range2210147 1 140 980 annotation2210146 1 RBS range2210146 1 128 133 BBa_K814000 1 BBa_K814000 dehydroquinate synthase (DHQS) generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1) Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli. false false _1071_ 0 9070 9 Not in stock false Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites. false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn annotation2210139 1 RBS range2210139 1 128 133 annotation2210141 1 lacP' range2210141 1 1 127 annotation2210140 1 DHQS range2210140 1 140 1373 BBa_K814001_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatggcacaatcccttcccctttcttccgcacctgctacaccgtctcttccttcccagacgaaaatagccgcaattatccaaaatatctgcactttggctttgttattactagcattgcccattaatgccaccattgtttttatatccttgttagtcttccgaccgcaaaaggtcaaagcagcaaacccccaaaccattcttatcagtggcggtaagatgaccaaagctttacaactagcaaggtcattccacgcggctggacatagagttgtcttggtggaaacccataaatactggttgactggtcatcgtttttcccaagcagtggataagttttacacagtccccgcaccccaggacaatccccaagcttacattcaggctttggtagatatcgtcaaacaagaaaacatcgatgtttatattcccgtcaccagtccagtgggtagctactacgactcattagccaaaccagagttatcccattattgcgaagtgtttcactttgacgcagatattacccaaatgttggatgataaatttgcgttgacacaaaaagcgcgatcgcttggtttatcagtacccaaatcctttaaaattacctcaccagaacaagtcatcaacttcgatttttctggagagacacgtaaatacatcctcaaaagcattccctacgactcagtgcggcggttggacttaaccaaactcccctgtgctactccagaggaaacagcagcattcgtcagaagtttgccaattactcccgaaaaaccgtggattatgcaggaatttatccccggtaaggaattctgcacccatagcaccgttcggaatggggaactcagactgcattgctgttgcgaatcttcagccttccaagttaattatgagaatgtaaataacccgcaaattaccgaatgggtacagcattttgtcaaggaactgaaactgacaggacagatttcctttgactttatccaagccgaagacggaacagtttacgccatcgagtgtaacccccgcacacattcagcaattaccacattttacgaccacccccaggtagcagaagcgtacttgagtcaagcaccgacgactgaaaccatacaaccactaacgacaagcaagcctacctattggacttatcacgaagtttggcgtttaactggtatccgttctttcacccagttgcaaagatggctggggaatatttggcgcgggactgatgcgatttatcagccagatgaccccttaccgtttttgatggtacatcattggcaaattcccctactgttattgaataatttgcgtcgtcttaaaggttggacgcggatagatttcaatattgggaagttggtggaattggggggagattaggcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg BBa_K814004_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgagtatcgtccaagcaaagtttgaagctaaggaaacatcttttcatgtagaaggttacgaaaagattgagtatgatttggtgtatgtagatggtatttttgaaatccagaattctgcactagcagatgtatatcaaggttttggacgatgcttggcgattgtagatgctaacgtcagtcggttgtatggtaatcaaattcaggcatatttccagtattatggtatagaactgaggctatttcctattaccattactgaaccagataagactattcaaactttcgagagagttatagatgtctttgcagatttcaaattagtccgcaaagaaccagtattagtcgtgggtggcggtttaattacagatgttgtcggctttgcttgttctacatatcgtcgcagcagcaattacatccgcattcctactacattgattggattaattgatgccagtgtagcaattaaggtagcagttaatcatcgcaaactgaaaaaccgtttgggtgcttatcatgcttctcgcaaagtatttttagatttctccttgttgcgtactctccctacagaccaagtacgtaacgggatggcggaattggtaaaaatcgctgtagtagcgcatcaagaagtttttgaattgttggagaagtacggcgaagaattactacgtactcattttggcaatatagatgcaactccagagattaaagaaatagcccatcgtttgacttacaaagctatccataagatgttggaattggaagttcccaacctgcatgagttagacctagatagggtgattgcttacggtcacacttggagtcccaccttggaacttgcgcctcgtctacccatgttccacggacacgccgttaatgtagatatggctttctcggcaacgatcgccgcccgtagaggatatattacaattgcagaacgcgatcgtattttaggattaatgagtcgcgttggtctatccctcgaccatcccatgttggatatagatattttgtggcgtggtactgaatctatcacattaactcgtgatggtttgttaagagctgctatgccaaaacccattggtgattgtgtcttcgtcaatgacctgacaagagaagaattagcagccgcattagctgaccacaaagaactttgtaccagttatccccgtggtggtgaaggtgtggatgtgtatcccgtttatcaaaaagaattaatcgggagtgttaaataagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagagcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatggcacaatcccttcccctttcttccgcacctgctacaccgtctcttccttcccagacgaaaatagccgcaattatccaaaatatctgcactttggctttgttattactagcattgcccattaatgccaccattgtttttatatccttgttagtcttccgaccgcaaaaggtcaaagcagcaaacccccaaaccattcttatcagtggcggtaagatgaccaaagctttacaactagcaaggtcattccacgcggctggacatagagttgtcttggtggaaacccataaatactggttgactggtcatcgtttttcccaagcagtggataagttttacacagtccccgcaccccaggacaatccccaagcttacattcaggctttggtagatatcgtcaaacaagaaaacatcgatgtttatattcccgtcaccagtccagtgggtagctactacgactcattagccaaaccagagttatcccattattgcgaagtgtttcactttgacgcagatattacccaaatgttggatgataaatttgcgttgacacaaaaagcgcgatcgcttggtttatcagtacccaaatcctttaaaattacctcaccagaacaagtcatcaacttcgatttttctggagagacacgtaaatacatcctcaaaagcattccctacgactcagtgcggcggttggacttaaccaaactcccctgtgctactccagaggaaacagcagcattcgtcagaagtttgccaattactcccgaaaaaccgtggattatgcaggaatttatccccggtaaggaattctgcacccatagcaccgttcggaatggggaactcagactgcattgctgttgcgaatcttcagccttccaagttaattatgagaatgtaaataacccgcaaattaccgaatgggtacagcattttgtcaaggaactgaaactgacaggacagatttcctttgactttatccaagccgaagacggaacagtttacgccatcgagtgtaacccccgcacacattcagcaattaccacattttacgaccacccccaggtagcagaagcgtacttgagtcaagcaccgacgactgaaaccatacaaccactaacgacaagcaagcctacctattggacttatcacgaagtttggcgtttaactggtatccgttctttcacccagttgcaaagatggctggggaatatttggcgcgggactgatgcgatttatcagccagatgaccccttaccgtttttgatggtacatcattggcaaattcccctactgttattgaataatttgcgtcgtcttaaaggttggacgcggatagatttcaatattgggaagttggtggaattggggggagattaggcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagagcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgacaaatgtgattgtccaaccaacagctagacctgttacaccattgggaattttaaccaagcagttagaagccatagtccaagaggttaagcaacatccagatttacctggggaattgatagcaaacatccatcaggcttggcgtttagccgcaggtatagacccttatttggaagaatgcaccactccagaatctcctgaactcgctgcattggcaaaaaccacagccaccgaagcctggggagaacacttccacggaggtacaaccgtccgtcctctagaacaagagatgctttctggtcatatcgaaggacaaaccttaaagatgtttgttcacatgaccaaagctaaaaaagtcttagaaattgggatgtttaccggttattcggcgctggcgatggcggaagcattaccagaggatggactgcttgtggcttgtgaagttgacccttacgcggcggaaattggacagaaagcctttcaacaatctccccacggtggaaagattcgtgtggaattggatgcagccttagcaactcttgataagttagcagaagctggggagtcttttgacttggtatttatcgacgcagataaaaaagagtatgtagcctattttcacaagttgctaggtagcagtttgttagcaccagatggctttatttgtgtagataacaccttattacaaggggaagtttatctaccagcagaggaacgtagcgtcaatggtgaagcgatcgcgcaatttaatcatacagtagctatagacccccgtgtagaacaggttttgttgccgttgcgagatggtttaacaattatccgcagaatacaaccttaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg BBa_K814000_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgagtatcgtccaagcaaagtttgaagctaaggaaacatcttttcatgtagaaggttacgaaaagattgagtatgatttggtgtatgtagatggtatttttgaaatccagaattctgcactagcagatgtatatcaaggttttggacgatgcttggcgattgtagatgctaacgtcagtcggttgtatggtaatcaaattcaggcatatttccagtattatggtatagaactgaggctatttcctattaccattactgaaccagataagactattcaaactttcgagagagttatagatgtctttgcagatttcaaattagtccgcaaagaaccagtattagtcgtgggtggcggtttaattacagatgttgtcggctttgcttgttctacatatcgtcgcagcagcaattacatccgcattcctactacattgattggattaattgatgccagtgtagcaattaaggtagcagttaatcatcgcaaactgaaaaaccgtttgggtgcttatcatgcttctcgcaaagtatttttagatttctccttgttgcgtactctccctacagaccaagtacgtaacgggatggcggaattggtaaaaatcgctgtagtagcgcatcaagaagtttttgaattgttggagaagtacggcgaagaattactacgtactcattttggcaatatagatgcaactccagagattaaagaaatagcccatcgtttgacttacaaagctatccataagatgttggaattggaagttcccaacctgcatgagttagacctagatagggtgattgcttacggtcacacttggagtcccaccttggaacttgcgcctcgtctacccatgttccacggacacgccgttaatgtagatatggctttctcggcaacgatcgccgcccgtagaggatatattacaattgcagaacgcgatcgtattttaggattaatgagtcgcgttggtctatccctcgaccatcccatgttggatatagatattttgtggcgtggtactgaatctatcacattaactcgtgatggtttgttaagagctgctatgccaaaacccattggtgattgtgtcttcgtcaatgacctgacaagagaagaattagcagccgcattagctgaccacaaagaactttgtaccagttatccccgtggtggtgaaggtgtggatgtgtatcccgtttatcaaaaagaattaatcgggagtgttaaataagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg BBa_K814002_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgacaaatgtgattgtccaaccaacagctagacctgttacaccattgggaattttaaccaagcagttagaagccatagtccaagaggttaagcaacatccagatttacctggggaattgatagcaaacatccatcaggcttggcgtttagccgcaggtatagacccttatttggaagaatgcaccactccagaatctcctgaactcgctgcattggcaaaaaccacagccaccgaagcctggggagaacacttccacggaggtacaaccgtccgtcctctagaacaagagatgctttctggtcatatcgaaggacaaaccttaaagatgtttgttcacatgaccaaagctaaaaaagtcttagaaattgggatgtttaccggttattcggcgctggcgatggcggaagcattaccagaggatggactgcttgtggcttgtgaagttgacccttacgcggcggaaattggacagaaagcctttcaacaatctccccacggtggaaagattcgtgtggaattggatgcagccttagcaactcttgataagttagcagaagctggggagtcttttgacttggtatttatcgacgcagataaaaaagagtatgtagcctattttcacaagttgctaggtagcagtttgttagcaccagatggctttatttgtgtagataacaccttattacaaggggaagtttatctaccagcagaggaacgtagcgtcaatggtgaagcgatcgcgcaatttaatcatacagtagctatagacccccgtgtagaacaggttttgttgccgttgcgagatggtttaacaattatccgcagaatacaaccttaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z