BBa_K814000
1
BBa_K814000
dehydroquinate synthase (DHQS) generator
2012-10-02T11:00:00Z
2015-05-08T01:13:28Z
Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1)
Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate.
This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli.
false
false
_1071_
0
9070
9
Not in stock
false
Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites.
false
Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn
annotation2210139
1
RBS
range2210139
1
128
133
annotation2210141
1
lacP'
range2210141
1
1
127
annotation2210140
1
DHQS
range2210140
1
140
1373
BBa_K814001
1
BBa_K814001
ATP-grasp (ATPG) generator
2012-10-02T11:00:00Z
2015-05-08T01:13:28Z
Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1)
Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. ATP-grasp (ATPG) catalyzes the third step in this pathway, 4-deoxygadusol into mycosporine-glycine.
This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli.
false
false
_1071_
0
9070
9
It's complicated
false
Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites.
false
Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn
annotation2210144
1
ATPG
range2210144
1
140
1517
annotation2210142
1
lacP'
range2210142
1
1
127
annotation2210143
1
RBS
range2210143
1
128
133
BBa_K814002
1
BBa_K814002
dehydroquinate O-methyltrasferase (O-MT) generator
2012-10-02T11:00:00Z
2015-05-08T01:13:28Z
Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1)
Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. Dehydroquinate O-methyltransferase (O-MT) catalyzes the second step in this pathway, converting dehydroquinate to 4-deoxygadusol.
This part includes a modified constitutive lac promoter (lacPmod), and RBS and the open reading frame of O-MT. This part can be used to express O-MT in E. coli.
false
false
_1071_
0
9070
9
Not in stock
false
Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites.
false
Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn
annotation2210147
1
O-MT
range2210147
1
140
980
annotation2210145
1
lacP'
range2210145
1
1
127
annotation2210146
1
RBS
range2210146
1
128
133
BBa_K814004
1
BBa_K814004
mycosporine-glycine biosynthetic pathway
2012-10-02T11:00:00Z
2015-05-08T01:13:28Z
All open reading frames in this composite part were taken from Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1).
Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. Dehydroquinate-O-methyltransferase (O-MT) then generates 4-deoxygadusol, which is converted to mycosprine-glycine by ATP-grasp (ATPG).
This composite part contains protein generators for DHQS, O-MT and ATPG. E. coli transformed with this composite part are able to synthesize mycosporine-glycine, as detected by HPLC analysis of the media. 4-deoxygadusol is also detected in HPLC analysis.
false
false
_1071_
0
9070
9
Not in stock
false
Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota)for A. variabilis. From here, PCR primer extension was used to clone each individual gene with flanking restriction digest sites to be used for vector cloning. Each gene was cloned into an individual pUCBB BioBrick??? cloning vectors containing the lacP' promoter and an RBS. The resulting generators were then stacked into a single BioBrick??? cloning vector using classic assembly techniques
false
Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn
component2210158
1
BBa_K814001
component2210162
1
BBa_K814002
component2210154
1
BBa_K814000
annotation2210154
1
BBa_K814000
range2210154
1
1
1445
annotation2210158
1
BBa_K814001
range2210158
1
1454
3042
annotation2210162
1
BBa_K814002
range2210162
1
3051
4102
BBa_K814001_sequence
1
cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatggcacaatcccttcccctttcttccgcacctgctacaccgtctcttccttcccagacgaaaatagccgcaattatccaaaatatctgcactttggctttgttattactagcattgcccattaatgccaccattgtttttatatccttgttagtcttccgaccgcaaaaggtcaaagcagcaaacccccaaaccattcttatcagtggcggtaagatgaccaaagctttacaactagcaaggtcattccacgcggctggacatagagttgtcttggtggaaacccataaatactggttgactggtcatcgtttttcccaagcagtggataagttttacacagtccccgcaccccaggacaatccccaagcttacattcaggctttggtagatatcgtcaaacaagaaaacatcgatgtttatattcccgtcaccagtccagtgggtagctactacgactcattagccaaaccagagttatcccattattgcgaagtgtttcactttgacgcagatattacccaaatgttggatgataaatttgcgttgacacaaaaagcgcgatcgcttggtttatcagtacccaaatcctttaaaattacctcaccagaacaagtcatcaacttcgatttttctggagagacacgtaaatacatcctcaaaagcattccctacgactcagtgcggcggttggacttaaccaaactcccctgtgctactccagaggaaacagcagcattcgtcagaagtttgccaattactcccgaaaaaccgtggattatgcaggaatttatccccggtaaggaattctgcacccatagcaccgttcggaatggggaactcagactgcattgctgttgcgaatcttcagccttccaagttaattatgagaatgtaaataacccgcaaattaccgaatgggtacagcattttgtcaaggaactgaaactgacaggacagatttcctttgactttatccaagccgaagacggaacagtttacgccatcgagtgtaacccccgcacacattcagcaattaccacattttacgaccacccccaggtagcagaagcgtacttgagtcaagcaccgacgactgaaaccatacaaccactaacgacaagcaagcctacctattggacttatcacgaagtttggcgtttaactggtatccgttctttcacccagttgcaaagatggctggggaatatttggcgcgggactgatgcgatttatcagccagatgaccccttaccgtttttgatggtacatcattggcaaattcccctactgttattgaataatttgcgtcgtcttaaaggttggacgcggatagatttcaatattgggaagttggtggaattggggggagattaggcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg
BBa_K814004_sequence
1
cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgagtatcgtccaagcaaagtttgaagctaaggaaacatcttttcatgtagaaggttacgaaaagattgagtatgatttggtgtatgtagatggtatttttgaaatccagaattctgcactagcagatgtatatcaaggttttggacgatgcttggcgattgtagatgctaacgtcagtcggttgtatggtaatcaaattcaggcatatttccagtattatggtatagaactgaggctatttcctattaccattactgaaccagataagactattcaaactttcgagagagttatagatgtctttgcagatttcaaattagtccgcaaagaaccagtattagtcgtgggtggcggtttaattacagatgttgtcggctttgcttgttctacatatcgtcgcagcagcaattacatccgcattcctactacattgattggattaattgatgccagtgtagcaattaaggtagcagttaatcatcgcaaactgaaaaaccgtttgggtgcttatcatgcttctcgcaaagtatttttagatttctccttgttgcgtactctccctacagaccaagtacgtaacgggatggcggaattggtaaaaatcgctgtagtagcgcatcaagaagtttttgaattgttggagaagtacggcgaagaattactacgtactcattttggcaatatagatgcaactccagagattaaagaaatagcccatcgtttgacttacaaagctatccataagatgttggaattggaagttcccaacctgcatgagttagacctagatagggtgattgcttacggtcacacttggagtcccaccttggaacttgcgcctcgtctacccatgttccacggacacgccgttaatgtagatatggctttctcggcaacgatcgccgcccgtagaggatatattacaattgcagaacgcgatcgtattttaggattaatgagtcgcgttggtctatccctcgaccatcccatgttggatatagatattttgtggcgtggtactgaatctatcacattaactcgtgatggtttgttaagagctgctatgccaaaacccattggtgattgtgtcttcgtcaatgacctgacaagagaagaattagcagccgcattagctgaccacaaagaactttgtaccagttatccccgtggtggtgaaggtgtggatgtgtatcccgtttatcaaaaagaattaatcgggagtgttaaataagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagagcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatggcacaatcccttcccctttcttccgcacctgctacaccgtctcttccttcccagacgaaaatagccgcaattatccaaaatatctgcactttggctttgttattactagcattgcccattaatgccaccattgtttttatatccttgttagtcttccgaccgcaaaaggtcaaagcagcaaacccccaaaccattcttatcagtggcggtaagatgaccaaagctttacaactagcaaggtcattccacgcggctggacatagagttgtcttggtggaaacccataaatactggttgactggtcatcgtttttcccaagcagtggataagttttacacagtccccgcaccccaggacaatccccaagcttacattcaggctttggtagatatcgtcaaacaagaaaacatcgatgtttatattcccgtcaccagtccagtgggtagctactacgactcattagccaaaccagagttatcccattattgcgaagtgtttcactttgacgcagatattacccaaatgttggatgataaatttgcgttgacacaaaaagcgcgatcgcttggtttatcagtacccaaatcctttaaaattacctcaccagaacaagtcatcaacttcgatttttctggagagacacgtaaatacatcctcaaaagcattccctacgactcagtgcggcggttggacttaaccaaactcccctgtgctactccagaggaaacagcagcattcgtcagaagtttgccaattactcccgaaaaaccgtggattatgcaggaatttatccccggtaaggaattctgcacccatagcaccgttcggaatggggaactcagactgcattgctgttgcgaatcttcagccttccaagttaattatgagaatgtaaataacccgcaaattaccgaatgggtacagcattttgtcaaggaactgaaactgacaggacagatttcctttgactttatccaagccgaagacggaacagtttacgccatcgagtgtaacccccgcacacattcagcaattaccacattttacgaccacccccaggtagcagaagcgtacttgagtcaagcaccgacgactgaaaccatacaaccactaacgacaagcaagcctacctattggacttatcacgaagtttggcgtttaactggtatccgttctttcacccagttgcaaagatggctggggaatatttggcgcgggactgatgcgatttatcagccagatgaccccttaccgtttttgatggtacatcattggcaaattcccctactgttattgaataatttgcgtcgtcttaaaggttggacgcggatagatttcaatattgggaagttggtggaattggggggagattaggcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagagcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgacaaatgtgattgtccaaccaacagctagacctgttacaccattgggaattttaaccaagcagttagaagccatagtccaagaggttaagcaacatccagatttacctggggaattgatagcaaacatccatcaggcttggcgtttagccgcaggtatagacccttatttggaagaatgcaccactccagaatctcctgaactcgctgcattggcaaaaaccacagccaccgaagcctggggagaacacttccacggaggtacaaccgtccgtcctctagaacaagagatgctttctggtcatatcgaaggacaaaccttaaagatgtttgttcacatgaccaaagctaaaaaagtcttagaaattgggatgtttaccggttattcggcgctggcgatggcggaagcattaccagaggatggactgcttgtggcttgtgaagttgacccttacgcggcggaaattggacagaaagcctttcaacaatctccccacggtggaaagattcgtgtggaattggatgcagccttagcaactcttgataagttagcagaagctggggagtcttttgacttggtatttatcgacgcagataaaaaagagtatgtagcctattttcacaagttgctaggtagcagtttgttagcaccagatggctttatttgtgtagataacaccttattacaaggggaagtttatctaccagcagaggaacgtagcgtcaatggtgaagcgatcgcgcaatttaatcatacagtagctatagacccccgtgtagaacaggttttgttgccgttgcgagatggtttaacaattatccgcagaatacaaccttaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg
BBa_K814000_sequence
1
cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgagtatcgtccaagcaaagtttgaagctaaggaaacatcttttcatgtagaaggttacgaaaagattgagtatgatttggtgtatgtagatggtatttttgaaatccagaattctgcactagcagatgtatatcaaggttttggacgatgcttggcgattgtagatgctaacgtcagtcggttgtatggtaatcaaattcaggcatatttccagtattatggtatagaactgaggctatttcctattaccattactgaaccagataagactattcaaactttcgagagagttatagatgtctttgcagatttcaaattagtccgcaaagaaccagtattagtcgtgggtggcggtttaattacagatgttgtcggctttgcttgttctacatatcgtcgcagcagcaattacatccgcattcctactacattgattggattaattgatgccagtgtagcaattaaggtagcagttaatcatcgcaaactgaaaaaccgtttgggtgcttatcatgcttctcgcaaagtatttttagatttctccttgttgcgtactctccctacagaccaagtacgtaacgggatggcggaattggtaaaaatcgctgtagtagcgcatcaagaagtttttgaattgttggagaagtacggcgaagaattactacgtactcattttggcaatatagatgcaactccagagattaaagaaatagcccatcgtttgacttacaaagctatccataagatgttggaattggaagttcccaacctgcatgagttagacctagatagggtgattgcttacggtcacacttggagtcccaccttggaacttgcgcctcgtctacccatgttccacggacacgccgttaatgtagatatggctttctcggcaacgatcgccgcccgtagaggatatattacaattgcagaacgcgatcgtattttaggattaatgagtcgcgttggtctatccctcgaccatcccatgttggatatagatattttgtggcgtggtactgaatctatcacattaactcgtgatggtttgttaagagctgctatgccaaaacccattggtgattgtgtcttcgtcaatgacctgacaagagaagaattagcagccgcattagctgaccacaaagaactttgtaccagttatccccgtggtggtgaaggtgtggatgtgtatcccgtttatcaaaaagaattaatcgggagtgttaaataagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg
BBa_K814002_sequence
1
cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgacaaatgtgattgtccaaccaacagctagacctgttacaccattgggaattttaaccaagcagttagaagccatagtccaagaggttaagcaacatccagatttacctggggaattgatagcaaacatccatcaggcttggcgtttagccgcaggtatagacccttatttggaagaatgcaccactccagaatctcctgaactcgctgcattggcaaaaaccacagccaccgaagcctggggagaacacttccacggaggtacaaccgtccgtcctctagaacaagagatgctttctggtcatatcgaaggacaaaccttaaagatgtttgttcacatgaccaaagctaaaaaagtcttagaaattgggatgtttaccggttattcggcgctggcgatggcggaagcattaccagaggatggactgcttgtggcttgtgaagttgacccttacgcggcggaaattggacagaaagcctttcaacaatctccccacggtggaaagattcgtgtggaattggatgcagccttagcaactcttgataagttagcagaagctggggagtcttttgacttggtatttatcgacgcagataaaaaagagtatgtagcctattttcacaagttgctaggtagcagtttgttagcaccagatggctttatttgtgtagataacaccttattacaaggggaagtttatctaccagcagaggaacgtagcgtcaatggtgaagcgatcgcgcaatttaatcatacagtagctatagacccccgtgtagaacaggttttgttgccgttgcgagatggtttaacaattatccgcagaatacaaccttaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z