LOCUS BBa_K814005 6990 bp DNA linear 23-Nov-2024 DEFINITION shinorine biosynthetic pathway ACCESSION BBa_K814005 VERSION BBa_K814005.1 FEATURES Location/Qualifiers RBS 128..133 /label=RBS CDS 140..1373 /label=DHQS promoter 1..127 /label=lacP' misc_feature 1..1445 /label=BBa_K814000 promoter 1454..1580 /label=lacP' RBS 1581..1586 /label=RBS CDS 1593..2970 /label=ATPG misc_feature 1454..3042 /label=BBa_K814001 promoter 3051..3177 /label=lacP' RBS 3178..3183 /label=RBS CDS 3190..4030 /label=O-MT misc_feature 3051..4102 /label=BBa_K814002 promoter 4111..4237 /label=lacP' RBS 4238..4243 /label=RBS CDS 4250..6916 /label=NRPS misc_feature 4111..6990 /label=BBa_K814003 ORIGIN 1 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 61 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgtc 121 tagtagaagg aggagatcta tgagtatcgt ccaagcaaag tttgaagcta aggaaacatc 181 ttttcatgta gaaggttacg aaaagattga gtatgatttg gtgtatgtag atggtatttt 241 tgaaatccag aattctgcac tagcagatgt atatcaaggt tttggacgat gcttggcgat 301 tgtagatgct aacgtcagtc ggttgtatgg taatcaaatt caggcatatt tccagtatta 361 tggtatagaa ctgaggctat ttcctattac cattactgaa ccagataaga ctattcaaac 421 tttcgagaga gttatagatg tctttgcaga tttcaaatta gtccgcaaag aaccagtatt 481 agtcgtgggt ggcggtttaa ttacagatgt tgtcggcttt gcttgttcta catatcgtcg 541 cagcagcaat tacatccgca ttcctactac attgattgga ttaattgatg ccagtgtagc 601 aattaaggta gcagttaatc atcgcaaact gaaaaaccgt ttgggtgctt atcatgcttc 661 tcgcaaagta tttttagatt tctccttgtt gcgtactctc cctacagacc aagtacgtaa 721 cgggatggcg gaattggtaa aaatcgctgt agtagcgcat caagaagttt ttgaattgtt 781 ggagaagtac ggcgaagaat tactacgtac tcattttggc aatatagatg caactccaga 841 gattaaagaa atagcccatc gtttgactta caaagctatc cataagatgt tggaattgga 901 agttcccaac ctgcatgagt tagacctaga tagggtgatt gcttacggtc acacttggag 961 tcccaccttg gaacttgcgc ctcgtctacc catgttccac ggacacgccg ttaatgtaga 1021 tatggctttc tcggcaacga tcgccgcccg tagaggatat attacaattg cagaacgcga 1081 tcgtatttta ggattaatga gtcgcgttgg tctatccctc gaccatccca tgttggatat 1141 agatattttg tggcgtggta ctgaatctat cacattaact cgtgatggtt tgttaagagc 1201 tgctatgcca aaacccattg gtgattgtgt cttcgtcaat gacctgacaa gagaagaatt 1261 agcagccgca ttagctgacc acaaagaact ttgtaccagt tatccccgtg gtggtgaagg 1321 tgtggatgtg tatcccgttt atcaaaaaga attaatcggg agtgttaaat aagcggccgc 1381 ctcgagcatc accatcacca tcactgattc tccttacgca tctgtgcggt atttcacacc 1441 gcatgtacta gagcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 1501 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 1561 aattgtgagc gtctagtaga aggaggagat ctatggcaca atcccttccc ctttcttccg 1621 cacctgctac accgtctctt ccttcccaga cgaaaatagc cgcaattatc caaaatatct 1681 gcactttggc tttgttatta ctagcattgc ccattaatgc caccattgtt tttatatcct 1741 tgttagtctt ccgaccgcaa aaggtcaaag cagcaaaccc ccaaaccatt cttatcagtg 1801 gcggtaagat gaccaaagct ttacaactag caaggtcatt ccacgcggct ggacatagag 1861 ttgtcttggt ggaaacccat aaatactggt tgactggtca tcgtttttcc caagcagtgg 1921 ataagtttta cacagtcccc gcaccccagg acaatcccca agcttacatt caggctttgg 1981 tagatatcgt caaacaagaa aacatcgatg tttatattcc cgtcaccagt ccagtgggta 2041 gctactacga ctcattagcc aaaccagagt tatcccatta ttgcgaagtg tttcactttg 2101 acgcagatat tacccaaatg ttggatgata aatttgcgtt gacacaaaaa gcgcgatcgc 2161 ttggtttatc agtacccaaa tcctttaaaa ttacctcacc agaacaagtc atcaacttcg 2221 atttttctgg agagacacgt aaatacatcc tcaaaagcat tccctacgac tcagtgcggc 2281 ggttggactt aaccaaactc ccctgtgcta ctccagagga aacagcagca ttcgtcagaa 2341 gtttgccaat tactcccgaa aaaccgtgga ttatgcagga atttatcccc ggtaaggaat 2401 tctgcaccca tagcaccgtt cggaatgggg aactcagact gcattgctgt tgcgaatctt 2461 cagccttcca agttaattat gagaatgtaa ataacccgca aattaccgaa tgggtacagc 2521 attttgtcaa ggaactgaaa ctgacaggac agatttcctt tgactttatc caagccgaag 2581 acggaacagt ttacgccatc gagtgtaacc cccgcacaca ttcagcaatt accacatttt 2641 acgaccaccc ccaggtagca gaagcgtact tgagtcaagc accgacgact gaaaccatac 2701 aaccactaac gacaagcaag cctacctatt ggacttatca cgaagtttgg cgtttaactg 2761 gtatccgttc tttcacccag ttgcaaagat ggctggggaa tatttggcgc gggactgatg 2821 cgatttatca gccagatgac cccttaccgt ttttgatggt acatcattgg caaattcccc 2881 tactgttatt gaataatttg cgtcgtctta aaggttggac gcggatagat ttcaatattg 2941 ggaagttggt ggaattgggg ggagattagg cggccgcctc gagcatcacc atcaccatca 3001 ctgattctcc ttacgcatct gtgcggtatt tcacaccgca tgtactagag cccgactgga 3061 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 3121 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgtc tagtagaagg 3181 aggagatcta tgacaaatgt gattgtccaa ccaacagcta gacctgttac accattggga 3241 attttaacca agcagttaga agccatagtc caagaggtta agcaacatcc agatttacct 3301 ggggaattga tagcaaacat ccatcaggct tggcgtttag ccgcaggtat agacccttat 3361 ttggaagaat gcaccactcc agaatctcct gaactcgctg cattggcaaa aaccacagcc 3421 accgaagcct ggggagaaca cttccacgga ggtacaaccg tccgtcctct agaacaagag 3481 atgctttctg gtcatatcga aggacaaacc ttaaagatgt ttgttcacat gaccaaagct 3541 aaaaaagtct tagaaattgg gatgtttacc ggttattcgg cgctggcgat ggcggaagca 3601 ttaccagagg atggactgct tgtggcttgt gaagttgacc cttacgcggc ggaaattgga 3661 cagaaagcct ttcaacaatc tccccacggt ggaaagattc gtgtggaatt ggatgcagcc 3721 ttagcaactc ttgataagtt agcagaagct ggggagtctt ttgacttggt atttatcgac 3781 gcagataaaa aagagtatgt agcctatttt cacaagttgc taggtagcag tttgttagca 3841 ccagatggct ttatttgtgt agataacacc ttattacaag gggaagttta tctaccagca 3901 gaggaacgta gcgtcaatgg tgaagcgatc gcgcaattta atcatacagt agctatagac 3961 ccccgtgtag aacaggtttt gttgccgttg cgagatggtt taacaattat ccgcagaata 4021 caaccttaag cggccgcctc gagcatcacc atcaccatca ctgattctcc ttacgcatct 4081 gtgcggtatt tcacaccgca tgtactagag cccgactgga aagcgggcag tgagcgcaac 4141 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 4201 gctcgtatgt tgtgtggaat tgtgagcgtc tagtagaagg aggagatcta tgcagactat 4261 agattttaat attcgtaagt tacttgtaga gtggaacgcg acccacagag attatgatct 4321 ttcccagagt ttacatgaac taattgtagc tcaagtagaa cgaacacctg aggcgatcgc 4381 tgtcaccttt gacaagcaac aactaactta tcaagaacta aatcataaag caaaccagct 4441 aggacattat ttacaaacat taggagtcca gccagaaacc ctggtaggcg tttgtttaga 4501 acgttcctta gaaatggtta tctgtctttt aggaatcctc aaagctgggg gtgcttatgt 4561 tcctattgac cctgaatatc ctcaagaacg catagcttat atgctagaag attctcaggt 4621 gaaggtacta ctaactcaag aaaaattact caatcaaatt ccccaccatc aagcacaaac 4681 tatctgtgta gatagggaat gggagaaaat ttccacacaa gctaatacca atcccaaaag 4741 taatataaaa acggataatc ttgcttatgt aatttacacc tctggttcca ctggtaaacc 4801 aaaaggtgca atgaacaccc acaaaggtat ctgtaatcgc ttattgtgga tgcaggaagc 4861 ttatcaaatc gattccacag atagcatttt acaaaaaacc ccctttagtt ttgatgtttc 4921 cgtttgggag ttcttttgga ctttattaac tggcgcacgt ttggtaatag ccaaaccagg 4981 cggacataaa gatagtgctt acctcatcga tttaattact caagaacaaa tcactacgtt 5041 gcattttgtc ccctcaatgc tgcaagtgtt tttacaaaat cgccatgtaa gcaaatgcag 5101 ctctctaaaa agagttattt gtagcggtga agctttatct atagatttac aaaatagatt 5161 tttccagcat ttgcaatgtg aattacataa cctctatggc ccgacagaag cagcaattga 5221 tgtcacattt tggcaatgta gaaaagatag taatttaaag agtgtaccta ttggtcgtcc 5281 cattgctaat actcaaattt atattcttga tgccgattta caaccagtaa atattggtgt 5341 cactggtgaa atttatattg gtggtgtagg ggttgctcgt ggttatttga ataaagaaga 5401 attgaccaaa gaaaaattta ttattaatcc ctttcccaat tctgagttta agcgacttta 5461 taaaacaggt gatttagctc gttatttacc cgatggaaat attgaatatc ttggtagaac 5521 agattatcaa gtaaaaattc ggggttatag aattgaaatt ggcgagattg aaaatgtttt 5581 atcttcacac ccacaagtca gagaagctgt agtcatagcg cgggatgata acgctcaaga 5641 aaaacaaatc atcgcttata ttacctataa ctccatcaaa cctcagcttg ataatctgcg 5701 tgatttccta aaagcaaggc tacctgattt tatgattcca gccgcttttg tgatgctgga 5761 gcatcttcct ttaactccca gtggtaaagt agaccgtaag gcattaccta agcctgattt 5821 atttaattat agtgaacata attcctatgt agcgcctcgg aatgaagttg aagaaaaatt 5881 agtacaaatc tggtcgaata ttctgcattt acctaaagta ggtgtgacag aaaacttttt 5941 cgctattggt ggtaattccc tcaaagctct acatttaatt tctcaaattg aagagttatt 6001 tgctaaagag atatccttag caacactttt aacaaatcca gtaattgcag atttagccaa 6061 ggttattcaa gcaaacaacc aaatccataa ttcaccccta gttccaattc aaccacaagg 6121 taagcagcag cctttctttt gtatacatcc tgctggtggt catgttttat gctattttaa 6181 actcgcacaa tatataggaa ctgaccaacc attttatggc ttacaagctc aaggatttta 6241 tggagatgaa gcacccttga cgcgagttga agatatggct agtctctacg tcaaaactat 6301 tagagaattt caaccccaag ggccttatcg tgtcgggggg tggtcatttg gtggagtcgt 6361 agcttatgaa gtagcacagc agttacatag acaaggacaa gaagtatctt tactagcaat 6421 attagattct tacgtaccga ttctgctgga taaacaaaaa cccattgatg acgtttattt 6481 agttggtgtt ctctccagag tttttggcgg tatgtttggt caagataatc tagtcacacc 6541 tgaagaaata gaaaatttaa ctgtagaaga aaaaattaat tacatcattg ataaagcacg 6601 gagcgctaga atattcccgc ctggtgtaga acgtcaaaat aatcgccgta ttcttgatgt 6661 tttggtggga actttaaaag caacttattc ctatataaga caaccatatc caggaaaagt 6721 cactgtattt cgagccaggg aaaaacatat tatggctcct gacccgacct tagtttgggt 6781 agaattattt tctgtaatgg cggctcaaga aattaagatt attgatgtcc ctggaaacca 6841 ttattcgttt gttctagaac cccatgtaca ggttttagca cagcgtttac aagattgtct 6901 ggaaaataat tcataagcgg ccgcctcgag catcaccatc accatcactg attctcctta 6961 cgcatctgtg cggtatttca caccgcatgt //