BBa_K814000 1 BBa_K814000 dehydroquinate synthase (DHQS) generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1) Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli. false false _1071_ 0 9070 9 Not in stock false Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites. false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn annotation2210139 1 RBS range2210139 1 128 133 annotation2210141 1 lacP' range2210141 1 1 127 annotation2210140 1 DHQS range2210140 1 140 1373 BBa_K814006 1 BBa_K814006 negative control for mycosporine-like amino acid biosynthesis 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z All open reading frames contained within this part came from Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1). Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. Dehydroquinate-O-methyltransferase (O-MT) then generates 4-deoxygadusol, which is converted to mycosprine-glycine by ATP-grasp (ATPG). Finally, mycosporine-glycine is converted into shinorine by a non-ribosomal peptide synthase (NRPS). This composite part contains protein generators for DHQS and ATPG. The part is non-functional and was used by the 2012 Minnesota iGEM team as a negative control when testing biosynthesis of mycosporine-like amino acids by the parts BBa_K814004 and BBa_K814005. false false _1071_ 0 9070 9 Not in stock false Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone each individual gene (seven in total) with flanking restriction digest sites to be used for vector cloning. Each gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a modified constitutive lac promoter (lacP')and RBS. From here, the resulting generators were stacked into a BioBrick??? vector using classical assembly techniques. false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn component2210182 1 BBa_K814000 component2210186 1 BBa_K814001 annotation2210186 1 BBa_K814001 range2210186 1 1454 3042 annotation2210182 1 BBa_K814000 range2210182 1 1 1445 BBa_K814001 1 BBa_K814001 ATP-grasp (ATPG) generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1) Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. ATP-grasp (ATPG) catalyzes the third step in this pathway, 4-deoxygadusol into mycosporine-glycine. This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli. false false _1071_ 0 9070 9 It's complicated false Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites. false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn annotation2210144 1 ATPG range2210144 1 140 1517 annotation2210143 1 RBS range2210143 1 128 133 annotation2210142 1 lacP' range2210142 1 1 127 BBa_K814001_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatggcacaatcccttcccctttcttccgcacctgctacaccgtctcttccttcccagacgaaaatagccgcaattatccaaaatatctgcactttggctttgttattactagcattgcccattaatgccaccattgtttttatatccttgttagtcttccgaccgcaaaaggtcaaagcagcaaacccccaaaccattcttatcagtggcggtaagatgaccaaagctttacaactagcaaggtcattccacgcggctggacatagagttgtcttggtggaaacccataaatactggttgactggtcatcgtttttcccaagcagtggataagttttacacagtccccgcaccccaggacaatccccaagcttacattcaggctttggtagatatcgtcaaacaagaaaacatcgatgtttatattcccgtcaccagtccagtgggtagctactacgactcattagccaaaccagagttatcccattattgcgaagtgtttcactttgacgcagatattacccaaatgttggatgataaatttgcgttgacacaaaaagcgcgatcgcttggtttatcagtacccaaatcctttaaaattacctcaccagaacaagtcatcaacttcgatttttctggagagacacgtaaatacatcctcaaaagcattccctacgactcagtgcggcggttggacttaaccaaactcccctgtgctactccagaggaaacagcagcattcgtcagaagtttgccaattactcccgaaaaaccgtggattatgcaggaatttatccccggtaaggaattctgcacccatagcaccgttcggaatggggaactcagactgcattgctgttgcgaatcttcagccttccaagttaattatgagaatgtaaataacccgcaaattaccgaatgggtacagcattttgtcaaggaactgaaactgacaggacagatttcctttgactttatccaagccgaagacggaacagtttacgccatcgagtgtaacccccgcacacattcagcaattaccacattttacgaccacccccaggtagcagaagcgtacttgagtcaagcaccgacgactgaaaccatacaaccactaacgacaagcaagcctacctattggacttatcacgaagtttggcgtttaactggtatccgttctttcacccagttgcaaagatggctggggaatatttggcgcgggactgatgcgatttatcagccagatgaccccttaccgtttttgatggtacatcattggcaaattcccctactgttattgaataatttgcgtcgtcttaaaggttggacgcggatagatttcaatattgggaagttggtggaattggggggagattaggcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg BBa_K814006_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgagtatcgtccaagcaaagtttgaagctaaggaaacatcttttcatgtagaaggttacgaaaagattgagtatgatttggtgtatgtagatggtatttttgaaatccagaattctgcactagcagatgtatatcaaggttttggacgatgcttggcgattgtagatgctaacgtcagtcggttgtatggtaatcaaattcaggcatatttccagtattatggtatagaactgaggctatttcctattaccattactgaaccagataagactattcaaactttcgagagagttatagatgtctttgcagatttcaaattagtccgcaaagaaccagtattagtcgtgggtggcggtttaattacagatgttgtcggctttgcttgttctacatatcgtcgcagcagcaattacatccgcattcctactacattgattggattaattgatgccagtgtagcaattaaggtagcagttaatcatcgcaaactgaaaaaccgtttgggtgcttatcatgcttctcgcaaagtatttttagatttctccttgttgcgtactctccctacagaccaagtacgtaacgggatggcggaattggtaaaaatcgctgtagtagcgcatcaagaagtttttgaattgttggagaagtacggcgaagaattactacgtactcattttggcaatatagatgcaactccagagattaaagaaatagcccatcgtttgacttacaaagctatccataagatgttggaattggaagttcccaacctgcatgagttagacctagatagggtgattgcttacggtcacacttggagtcccaccttggaacttgcgcctcgtctacccatgttccacggacacgccgttaatgtagatatggctttctcggcaacgatcgccgcccgtagaggatatattacaattgcagaacgcgatcgtattttaggattaatgagtcgcgttggtctatccctcgaccatcccatgttggatatagatattttgtggcgtggtactgaatctatcacattaactcgtgatggtttgttaagagctgctatgccaaaacccattggtgattgtgtcttcgtcaatgacctgacaagagaagaattagcagccgcattagctgaccacaaagaactttgtaccagttatccccgtggtggtgaaggtgtggatgtgtatcccgtttatcaaaaagaattaatcgggagtgttaaataagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagagcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatggcacaatcccttcccctttcttccgcacctgctacaccgtctcttccttcccagacgaaaatagccgcaattatccaaaatatctgcactttggctttgttattactagcattgcccattaatgccaccattgtttttatatccttgttagtcttccgaccgcaaaaggtcaaagcagcaaacccccaaaccattcttatcagtggcggtaagatgaccaaagctttacaactagcaaggtcattccacgcggctggacatagagttgtcttggtggaaacccataaatactggttgactggtcatcgtttttcccaagcagtggataagttttacacagtccccgcaccccaggacaatccccaagcttacattcaggctttggtagatatcgtcaaacaagaaaacatcgatgtttatattcccgtcaccagtccagtgggtagctactacgactcattagccaaaccagagttatcccattattgcgaagtgtttcactttgacgcagatattacccaaatgttggatgataaatttgcgttgacacaaaaagcgcgatcgcttggtttatcagtacccaaatcctttaaaattacctcaccagaacaagtcatcaacttcgatttttctggagagacacgtaaatacatcctcaaaagcattccctacgactcagtgcggcggttggacttaaccaaactcccctgtgctactccagaggaaacagcagcattcgtcagaagtttgccaattactcccgaaaaaccgtggattatgcaggaatttatccccggtaaggaattctgcacccatagcaccgttcggaatggggaactcagactgcattgctgttgcgaatcttcagccttccaagttaattatgagaatgtaaataacccgcaaattaccgaatgggtacagcattttgtcaaggaactgaaactgacaggacagatttcctttgactttatccaagccgaagacggaacagtttacgccatcgagtgtaacccccgcacacattcagcaattaccacattttacgaccacccccaggtagcagaagcgtacttgagtcaagcaccgacgactgaaaccatacaaccactaacgacaagcaagcctacctattggacttatcacgaagtttggcgtttaactggtatccgttctttcacccagttgcaaagatggctggggaatatttggcgcgggactgatgcgatttatcagccagatgaccccttaccgtttttgatggtacatcattggcaaattcccctactgttattgaataatttgcgtcgtcttaaaggttggacgcggatagatttcaatattgggaagttggtggaattggggggagattaggcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg BBa_K814000_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgagtatcgtccaagcaaagtttgaagctaaggaaacatcttttcatgtagaaggttacgaaaagattgagtatgatttggtgtatgtagatggtatttttgaaatccagaattctgcactagcagatgtatatcaaggttttggacgatgcttggcgattgtagatgctaacgtcagtcggttgtatggtaatcaaattcaggcatatttccagtattatggtatagaactgaggctatttcctattaccattactgaaccagataagactattcaaactttcgagagagttatagatgtctttgcagatttcaaattagtccgcaaagaaccagtattagtcgtgggtggcggtttaattacagatgttgtcggctttgcttgttctacatatcgtcgcagcagcaattacatccgcattcctactacattgattggattaattgatgccagtgtagcaattaaggtagcagttaatcatcgcaaactgaaaaaccgtttgggtgcttatcatgcttctcgcaaagtatttttagatttctccttgttgcgtactctccctacagaccaagtacgtaacgggatggcggaattggtaaaaatcgctgtagtagcgcatcaagaagtttttgaattgttggagaagtacggcgaagaattactacgtactcattttggcaatatagatgcaactccagagattaaagaaatagcccatcgtttgacttacaaagctatccataagatgttggaattggaagttcccaacctgcatgagttagacctagatagggtgattgcttacggtcacacttggagtcccaccttggaacttgcgcctcgtctacccatgttccacggacacgccgttaatgtagatatggctttctcggcaacgatcgccgcccgtagaggatatattacaattgcagaacgcgatcgtattttaggattaatgagtcgcgttggtctatccctcgaccatcccatgttggatatagatattttgtggcgtggtactgaatctatcacattaactcgtgatggtttgttaagagctgctatgccaaaacccattggtgattgtgtcttcgtcaatgacctgacaagagaagaattagcagccgcattagctgaccacaaagaactttgtaccagttatccccgtggtggtgaaggtgtggatgtgtatcccgtttatcaaaaagaattaatcgggagtgttaaataagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z