BBa_K814008 1 BBa_K814008 ScyB (leucine dehydrogenase) generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Nostoc punctiforme PCC 73102, accession NC_010628.1 Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyB (NpR1275), an acetolactate synthetase, carries out the first step in scytonemin biosynthesis, converting L-tryptophan to alpha-keto-tryptophan. This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyB. This part can be used to express ScyB protein in E. coli. false false _1071_ 0 9070 9 Not in stock false N. punctiforme genomic DNA was obtained from ATCC. From here, PCR primer extension was used to clone the gene with flanking restriction digest sites to be used for vector cloning. The gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a constitutive modified lac promoter (lacP???) and an RBS. false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn annotation2210325 1 lacP' range2210325 1 1 127 annotation2210327 1 RBS range2210327 1 128 133 annotation2210328 1 ScyB range2210328 1 140 1201 BBa_K814008_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgctgctatttgaaactgttagagaaatgggtcacgaacaagttcttttttgtcatggtaaaaatcccgaaattaaggcaattattgccatccatgacacgaccttgggcccagcaatgggagctacaagactcatgccttatgtcaacgaagaagctgctttaaaagatgcacttcgtctcagtcggggcatgacttataaagctgcttgtgcaaacattccagctggtggtggcaaagcagttattattgctaatcctgaaaataaaacagatgatctgttgagagcctacggacgttttgttaacagcctcaatgggcgttttatcactggacaagatgtcaatattacccctgatgatgtccgaacaatcagccaagaaactaaccatgttgttggagtctcagaaaaatctggtgggcctgctcccatcacatcattaggagtttttctaggaattaaagctgctgtagagtctcgttggcagagcaaaagacttgatgggatgaaagttgcagttcaaggcttaggtaatgtcgggaaaaatctctgtcgccacttacatgagcatgatgtcaagctttttgttagcgatgtagatccagcaaaagcggaagaagcaaaacggctttttggcgcaactgttgtagaacctactgaaatttactctcttgatgtagatatcttttctccttgcgctctgggaggaattcttaacagtcatacgattcctttcctccaagctcagattattgctggtgcagctaataatcaattggagaatgaacaattacatagtcaaatgctcactaggaaagggattctttacagcccagattatgtaattaatgctggaggtctaatcaacgtctacaacgaaatgattggttatgacgaagaaaaagctttcaaacaagtgcataacatttatgacacgctattagcaatttttgatattgctaaagaacaaggagttactactaacgatgctgctaaacgattagcagaagaccgcatcagcaatagtaagcgcagcaagtctaaagcgatcgcagcttaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z