BBa_K814009
1
BBa_K814009
ScyC generator
2012-10-02T11:00:00Z
2015-05-08T01:13:28Z
Nostoc punctiforme PCC 73102, accession NC_010628.1
Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyC (NpR1276) is a protein of unknown function which converts 3-indole pyruvic acid to a ketoacid intermediate which spontaneously dimerizes to form scytonemin.
This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyC. This part can be used to express ScyC protein in E. coli.
false
false
_1071_
0
9070
9
It's complicated
false
N. punctiforme genomic DNA was obtained from ATCC. From here, PCR primer extension was used to clone the gene with flanking restriction digest sites to be used for vector cloning. The gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a constitutive modified lac promoter (lacP???) and an RBS.
false
Sarah Bloch
annotation2210570
1
RBS
range2210570
1
128
133
annotation2210569
1
lacP'
range2210569
1
1
127
annotation2210571
1
ScyC
range2210571
1
140
2014
BBa_K814009_sequence
1
cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgagtcaaaactatactggttccaactctcctctcatcactactgagccatacaaagaatttccagcaaatagatatgtagaatcttctcgtcagcttgatgaatcggagaataacgggggaactcagccttttttaaatgatgaaacaccagcaacgctctcagttgccgatgcgatcgctcagatgttggtaaatttgggagtaagctacgcttttggtgtagcgggtggtgcaatggccagcctttggggggcgctatcaaatagcagcatagaagtgttgaacttccgccatgaagctggagcagcctttgcagccaccgaagcacactttgccaataatcgccccactgtagtttttaccacagccgggccggggatcactaacgcgctgaccggattatttgcggctcgtggtgaaggcgcaaaggtaattttgttgtcggcttgcacctctgcaccgcaacgtggacgttgggcaattcaagaaaccagcacttatacattgcccagtgggggaatctttaccgcaggagcgctattcaactatgcaatcactattgaatctgcggctcaactaccgcagattttccgtaaacttgcgatggctttggcgcaaccaggcggatttgtcgctcatttgagcattcccaccggtgtgcagacaagtttagttgaggatgtagctttaccccaactagatgttactccgtttccgatgactgcttccaaagaagcgatcgctaaatcggtagagttattatcatctggcccctttgctatctgggttggtttcggtgcgcgtgacgcagcagaggaaatccttgaactcgctgaaaaaacgggagcagccgttatgtgttccccccgtggtaaaggtatcttccctgaagatcatccccagtttgttggtgtcacaggtttaggcggtcatgcttccgtcttaacttatatggaacagcaacctccactacgcacactcgtattaggaacccgccttggtgaaccgacttctttctggagttcggcgttagttccaaaagaaggtttcatccatgtagatattgacccagaagtcccaggtgtagcctatccacaggttgaaactttcggggttcggtctgatatcaaagcttttgtggaagagttattggagcaattaccggatgctccccgttccacaactttgtcgctacctaatccagaacgcaaagcaatcgaacctgcaccagaggtagattatccagtgcggccagaagtattgatggcagcaattcaaaagataattgttgaaggtagcgatgcaatagtcatggcggagtgtggtaactcatttacttggtcaacgcatctactccaatttaccgaaaccaatcgttatcgagtcagcaccggagtcggcgcaatgggtcacgccgttacaggagtattgggtgcagcattggcaagcaataccaaggcggtaggcattgtaggcgatggggcaatgctgatgaataacgaaatcagcacagccgtgaaatacaaaattccggcgatttggattgtcctcaacgatgcacgttacaacatgtgccatcaagggatgaaaatcttgggattaaagggcgcagatgccacacttccaccgacaaacttcgccatgattgctcgtggcatgggagcagaagcgctcgtggttgttagagagtcggatattgaagcggcattagaacaagcgatcgcatcatctgttccctttctcattgatgtcgtaattgacgctgatcgcccagcaccttctggtggacgtaataagagtttggctgcacaaggaattaaatcaagtccagctaaaaatggagccaagcaagtttcatttccaatggtttgagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z