BBa_K814009 1 BBa_K814009 ScyC generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Nostoc punctiforme PCC 73102, accession NC_010628.1 Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyC (NpR1276) is a protein of unknown function which converts 3-indole pyruvic acid to a ketoacid intermediate which spontaneously dimerizes to form scytonemin. This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyC. This part can be used to express ScyC protein in E. coli. false false _1071_ 0 9070 9 It's complicated false N. punctiforme genomic DNA was obtained from ATCC. From here, PCR primer extension was used to clone the gene with flanking restriction digest sites to be used for vector cloning. The gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a constitutive modified lac promoter (lacP???) and an RBS. false Sarah Bloch annotation2210569 1 lacP' range2210569 1 1 127 annotation2210570 1 RBS range2210570 1 128 133 annotation2210571 1 ScyC range2210571 1 140 2014 BBa_K814009_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgagtcaaaactatactggttccaactctcctctcatcactactgagccatacaaagaatttccagcaaatagatatgtagaatcttctcgtcagcttgatgaatcggagaataacgggggaactcagccttttttaaatgatgaaacaccagcaacgctctcagttgccgatgcgatcgctcagatgttggtaaatttgggagtaagctacgcttttggtgtagcgggtggtgcaatggccagcctttggggggcgctatcaaatagcagcatagaagtgttgaacttccgccatgaagctggagcagcctttgcagccaccgaagcacactttgccaataatcgccccactgtagtttttaccacagccgggccggggatcactaacgcgctgaccggattatttgcggctcgtggtgaaggcgcaaaggtaattttgttgtcggcttgcacctctgcaccgcaacgtggacgttgggcaattcaagaaaccagcacttatacattgcccagtgggggaatctttaccgcaggagcgctattcaactatgcaatcactattgaatctgcggctcaactaccgcagattttccgtaaacttgcgatggctttggcgcaaccaggcggatttgtcgctcatttgagcattcccaccggtgtgcagacaagtttagttgaggatgtagctttaccccaactagatgttactccgtttccgatgactgcttccaaagaagcgatcgctaaatcggtagagttattatcatctggcccctttgctatctgggttggtttcggtgcgcgtgacgcagcagaggaaatccttgaactcgctgaaaaaacgggagcagccgttatgtgttccccccgtggtaaaggtatcttccctgaagatcatccccagtttgttggtgtcacaggtttaggcggtcatgcttccgtcttaacttatatggaacagcaacctccactacgcacactcgtattaggaacccgccttggtgaaccgacttctttctggagttcggcgttagttccaaaagaaggtttcatccatgtagatattgacccagaagtcccaggtgtagcctatccacaggttgaaactttcggggttcggtctgatatcaaagcttttgtggaagagttattggagcaattaccggatgctccccgttccacaactttgtcgctacctaatccagaacgcaaagcaatcgaacctgcaccagaggtagattatccagtgcggccagaagtattgatggcagcaattcaaaagataattgttgaaggtagcgatgcaatagtcatggcggagtgtggtaactcatttacttggtcaacgcatctactccaatttaccgaaaccaatcgttatcgagtcagcaccggagtcggcgcaatgggtcacgccgttacaggagtattgggtgcagcattggcaagcaataccaaggcggtaggcattgtaggcgatggggcaatgctgatgaataacgaaatcagcacagccgtgaaatacaaaattccggcgatttggattgtcctcaacgatgcacgttacaacatgtgccatcaagggatgaaaatcttgggattaaagggcgcagatgccacacttccaccgacaaacttcgccatgattgctcgtggcatgggagcagaagcgctcgtggttgttagagagtcggatattgaagcggcattagaacaagcgatcgcatcatctgttccctttctcattgatgtcgtaattgacgctgatcgcccagcaccttctggtggacgtaataagagtttggctgcacaaggaattaaatcaagtccagctaaaaatggagccaagcaagtttcatttccaatggtttgagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z