BBa_K814008 1 BBa_K814008 ScyB (leucine dehydrogenase) generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Nostoc punctiforme PCC 73102, accession NC_010628.1 Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyB (NpR1275), an acetolactate synthetase, carries out the first step in scytonemin biosynthesis, converting L-tryptophan to alpha-keto-tryptophan. This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyB. This part can be used to express ScyB protein in E. coli. false false _1071_ 0 9070 9 Not in stock false N. punctiforme genomic DNA was obtained from ATCC. From here, PCR primer extension was used to clone the gene with flanking restriction digest sites to be used for vector cloning. The gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a constitutive modified lac promoter (lacP???) and an RBS. false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn annotation2210325 1 lacP' range2210325 1 1 127 annotation2210327 1 RBS range2210327 1 128 133 annotation2210328 1 ScyB range2210328 1 140 1201 BBa_K814007 1 BBa_K814007 ScyA (acetolactate synthase) generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Nostoc punctiforme PCC 73102 genomic DNA, accession NC_010628.1 Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyA (NpR1276), an acetolactate synthetase, carries out the first step in scytonemin biosynthesis, converting L-tryptophan to alpha-keto-tryptophan. This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyA. This part can be used to express ScyA protein in E. coli. false false _1071_ 0 9070 9 Not in stock false N. punctiforme genomic DNA was obtained from ATCC. From here, PCR primer extension was used to clone the gene with flanking restriction digest sites to be used for vector cloning. The gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a constitutive modified lac promoter (lacP???) and an RBS. false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn annotation2210318 1 lacP' range2210318 1 1 127 annotation2210319 1 RBS range2210319 1 128 133 annotation2210320 1 ScyA range2210320 1 140 1108 BBa_K814011 1 BBa_K814011 scytonemin biosynthetic pathway, scyBAC 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z All open reading frames contained within this part came from Nostoc punctiforme PCC 73102, accession NC_010628.1 Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyB first converts tryptophan into 3-indole pyruvic acid, which is converted into a ketoacid intermediate by ScyA. ScyC then converts the ketoacid into an indole compound which spontaneously forms scytonemin. This part is made of three basic parts and contains the scyB, scyA and scyC generators. This part could be used for scytonemin biosynthesis in E. coli false false _1071_ 0 9070 9 Not in stock false N. punctiforme genomic DNA was obtained from ATCC. From here, PCR primer extension was used to clone each gene with flanking restriction digest sites to be used for vector cloning. Each gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a constitutive modified lac promoter (lacP???) and an RBS. These basic parts were then assembled into the scyBAC pathway through standard BioBrick??? assembly techniques false Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn component2210691 1 BBa_K814007 component2210695 1 BBa_K814009 component2210687 1 BBa_K814008 annotation2210691 1 BBa_K814007 range2210691 1 1283 2463 annotation2210687 1 BBa_K814008 range2210687 1 1 1274 annotation2210695 1 BBa_K814009 range2210695 1 2472 4558 BBa_K814009 1 BBa_K814009 ScyC generator 2012-10-02T11:00:00Z 2015-05-08T01:13:28Z Nostoc punctiforme PCC 73102, accession NC_010628.1 Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyC (NpR1276) is a protein of unknown function which converts 3-indole pyruvic acid to a ketoacid intermediate which spontaneously dimerizes to form scytonemin. This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyC. This part can be used to express ScyC protein in E. coli. false false _1071_ 0 9070 9 It's complicated false N. punctiforme genomic DNA was obtained from ATCC. From here, PCR primer extension was used to clone the gene with flanking restriction digest sites to be used for vector cloning. The gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a constitutive modified lac promoter (lacP???) and an RBS. false Sarah Bloch annotation2210569 1 lacP' range2210569 1 1 127 annotation2210571 1 ScyC range2210571 1 140 2014 annotation2210570 1 RBS range2210570 1 128 133 BBa_K814008_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgctgctatttgaaactgttagagaaatgggtcacgaacaagttcttttttgtcatggtaaaaatcccgaaattaaggcaattattgccatccatgacacgaccttgggcccagcaatgggagctacaagactcatgccttatgtcaacgaagaagctgctttaaaagatgcacttcgtctcagtcggggcatgacttataaagctgcttgtgcaaacattccagctggtggtggcaaagcagttattattgctaatcctgaaaataaaacagatgatctgttgagagcctacggacgttttgttaacagcctcaatgggcgttttatcactggacaagatgtcaatattacccctgatgatgtccgaacaatcagccaagaaactaaccatgttgttggagtctcagaaaaatctggtgggcctgctcccatcacatcattaggagtttttctaggaattaaagctgctgtagagtctcgttggcagagcaaaagacttgatgggatgaaagttgcagttcaaggcttaggtaatgtcgggaaaaatctctgtcgccacttacatgagcatgatgtcaagctttttgttagcgatgtagatccagcaaaagcggaagaagcaaaacggctttttggcgcaactgttgtagaacctactgaaatttactctcttgatgtagatatcttttctccttgcgctctgggaggaattcttaacagtcatacgattcctttcctccaagctcagattattgctggtgcagctaataatcaattggagaatgaacaattacatagtcaaatgctcactaggaaagggattctttacagcccagattatgtaattaatgctggaggtctaatcaacgtctacaacgaaatgattggttatgacgaagaaaaagctttcaaacaagtgcataacatttatgacacgctattagcaatttttgatattgctaaagaacaaggagttactactaacgatgctgctaaacgattagcagaagaccgcatcagcaatagtaagcgcagcaagtctaaagcgatcgcagcttaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg BBa_K814007_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatggaaaaaaatacttttgcaacatcagcttacattgctacttcacctgagagtgcatttgaatatctttgtagtttaaagaacctggatgaatggactctttatagccggatgaaagagcaaattgatgaagatacctggctcggaactgcatcgggttatcacaaaaatctctattatcacgttaaaaaactccaaagtccgcttttctatggcattgaatggcactgtggattagagtatcaaaaatattttcaagtctatcctgttttgctgtttcccaccgactacatcgagccaggaacagatgaaaaaggggtgtattttcactggttgagctttgtcgatccaaaacggcagactcagatgattatgcaggggattcatacagtacacacttctgaatgtcgttctctcaaaggtaatttggaacgcaaagctggtctgacctcagcagctaaaggaagccactttatcgatactgataccatttatgttgatgccccaattgaaatcggtattgaatacttaaaagacttacaaaacatagatgagtgggcgcatctgttacgcccaaatggtgatattacttctgaatcaggtgaattcaaagatgaatacgatcaaaaagtcaaagtttccgtgagagttcattctttgagtaaatactatttacttgaacaagaatatttttatccagagtacgaatattatcagcgttctgtagcgttacttatcccaaccgcttatgctttcgctgaccccgaagcttctggtttcatcctgcatcgaatcacattctggaaaacagatggaactgttagccacggcaaacttcaaattgaagactttggcgctgagagcatgaacatcaaacgtttactcgaagccaaagctggcaacctcaaatcattcgacagaggaatgagctatctgccaaaaattcaagaatccctagttcccaactaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg BBa_K814009_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgagtcaaaactatactggttccaactctcctctcatcactactgagccatacaaagaatttccagcaaatagatatgtagaatcttctcgtcagcttgatgaatcggagaataacgggggaactcagccttttttaaatgatgaaacaccagcaacgctctcagttgccgatgcgatcgctcagatgttggtaaatttgggagtaagctacgcttttggtgtagcgggtggtgcaatggccagcctttggggggcgctatcaaatagcagcatagaagtgttgaacttccgccatgaagctggagcagcctttgcagccaccgaagcacactttgccaataatcgccccactgtagtttttaccacagccgggccggggatcactaacgcgctgaccggattatttgcggctcgtggtgaaggcgcaaaggtaattttgttgtcggcttgcacctctgcaccgcaacgtggacgttgggcaattcaagaaaccagcacttatacattgcccagtgggggaatctttaccgcaggagcgctattcaactatgcaatcactattgaatctgcggctcaactaccgcagattttccgtaaacttgcgatggctttggcgcaaccaggcggatttgtcgctcatttgagcattcccaccggtgtgcagacaagtttagttgaggatgtagctttaccccaactagatgttactccgtttccgatgactgcttccaaagaagcgatcgctaaatcggtagagttattatcatctggcccctttgctatctgggttggtttcggtgcgcgtgacgcagcagaggaaatccttgaactcgctgaaaaaacgggagcagccgttatgtgttccccccgtggtaaaggtatcttccctgaagatcatccccagtttgttggtgtcacaggtttaggcggtcatgcttccgtcttaacttatatggaacagcaacctccactacgcacactcgtattaggaacccgccttggtgaaccgacttctttctggagttcggcgttagttccaaaagaaggtttcatccatgtagatattgacccagaagtcccaggtgtagcctatccacaggttgaaactttcggggttcggtctgatatcaaagcttttgtggaagagttattggagcaattaccggatgctccccgttccacaactttgtcgctacctaatccagaacgcaaagcaatcgaacctgcaccagaggtagattatccagtgcggccagaagtattgatggcagcaattcaaaagataattgttgaaggtagcgatgcaatagtcatggcggagtgtggtaactcatttacttggtcaacgcatctactccaatttaccgaaaccaatcgttatcgagtcagcaccggagtcggcgcaatgggtcacgccgttacaggagtattgggtgcagcattggcaagcaataccaaggcggtaggcattgtaggcgatggggcaatgctgatgaataacgaaatcagcacagccgtgaaatacaaaattccggcgatttggattgtcctcaacgatgcacgttacaacatgtgccatcaagggatgaaaatcttgggattaaagggcgcagatgccacacttccaccgacaaacttcgccatgattgctcgtggcatgggagcagaagcgctcgtggttgttagagagtcggatattgaagcggcattagaacaagcgatcgcatcatctgttccctttctcattgatgtcgtaattgacgctgatcgcccagcaccttctggtggacgtaataagagtttggctgcacaaggaattaaatcaagtccagctaaaaatggagccaagcaagtttcatttccaatggtttgagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg BBa_K814011_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgctgctatttgaaactgttagagaaatgggtcacgaacaagttcttttttgtcatggtaaaaatcccgaaattaaggcaattattgccatccatgacacgaccttgggcccagcaatgggagctacaagactcatgccttatgtcaacgaagaagctgctttaaaagatgcacttcgtctcagtcggggcatgacttataaagctgcttgtgcaaacattccagctggtggtggcaaagcagttattattgctaatcctgaaaataaaacagatgatctgttgagagcctacggacgttttgttaacagcctcaatgggcgttttatcactggacaagatgtcaatattacccctgatgatgtccgaacaatcagccaagaaactaaccatgttgttggagtctcagaaaaatctggtgggcctgctcccatcacatcattaggagtttttctaggaattaaagctgctgtagagtctcgttggcagagcaaaagacttgatgggatgaaagttgcagttcaaggcttaggtaatgtcgggaaaaatctctgtcgccacttacatgagcatgatgtcaagctttttgttagcgatgtagatccagcaaaagcggaagaagcaaaacggctttttggcgcaactgttgtagaacctactgaaatttactctcttgatgtagatatcttttctccttgcgctctgggaggaattcttaacagtcatacgattcctttcctccaagctcagattattgctggtgcagctaataatcaattggagaatgaacaattacatagtcaaatgctcactaggaaagggattctttacagcccagattatgtaattaatgctggaggtctaatcaacgtctacaacgaaatgattggttatgacgaagaaaaagctttcaaacaagtgcataacatttatgacacgctattagcaatttttgatattgctaaagaacaaggagttactactaacgatgctgctaaacgattagcagaagaccgcatcagcaatagtaagcgcagcaagtctaaagcgatcgcagcttaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagagcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatggaaaaaaatacttttgcaacatcagcttacattgctacttcacctgagagtgcatttgaatatctttgtagtttaaagaacctggatgaatggactctttatagccggatgaaagagcaaattgatgaagatacctggctcggaactgcatcgggttatcacaaaaatctctattatcacgttaaaaaactccaaagtccgcttttctatggcattgaatggcactgtggattagagtatcaaaaatattttcaagtctatcctgttttgctgtttcccaccgactacatcgagccaggaacagatgaaaaaggggtgtattttcactggttgagctttgtcgatccaaaacggcagactcagatgattatgcaggggattcatacagtacacacttctgaatgtcgttctctcaaaggtaatttggaacgcaaagctggtctgacctcagcagctaaaggaagccactttatcgatactgataccatttatgttgatgccccaattgaaatcggtattgaatacttaaaagacttacaaaacatagatgagtgggcgcatctgttacgcccaaatggtgatattacttctgaatcaggtgaattcaaagatgaatacgatcaaaaagtcaaagtttccgtgagagttcattctttgagtaaatactatttacttgaacaagaatatttttatccagagtacgaatattatcagcgttctgtagcgttacttatcccaaccgcttatgctttcgctgaccccgaagcttctggtttcatcctgcatcgaatcacattctggaaaacagatggaactgttagccacggcaaacttcaaattgaagactttggcgctgagagcatgaacatcaaacgtttactcgaagccaaagctggcaacctcaaatcattcgacagaggaatgagctatctgccaaaaattcaagaatccctagttcccaactaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagagcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgagtcaaaactatactggttccaactctcctctcatcactactgagccatacaaagaatttccagcaaatagatatgtagaatcttctcgtcagcttgatgaatcggagaataacgggggaactcagccttttttaaatgatgaaacaccagcaacgctctcagttgccgatgcgatcgctcagatgttggtaaatttgggagtaagctacgcttttggtgtagcgggtggtgcaatggccagcctttggggggcgctatcaaatagcagcatagaagtgttgaacttccgccatgaagctggagcagcctttgcagccaccgaagcacactttgccaataatcgccccactgtagtttttaccacagccgggccggggatcactaacgcgctgaccggattatttgcggctcgtggtgaaggcgcaaaggtaattttgttgtcggcttgcacctctgcaccgcaacgtggacgttgggcaattcaagaaaccagcacttatacattgcccagtgggggaatctttaccgcaggagcgctattcaactatgcaatcactattgaatctgcggctcaactaccgcagattttccgtaaacttgcgatggctttggcgcaaccaggcggatttgtcgctcatttgagcattcccaccggtgtgcagacaagtttagttgaggatgtagctttaccccaactagatgttactccgtttccgatgactgcttccaaagaagcgatcgctaaatcggtagagttattatcatctggcccctttgctatctgggttggtttcggtgcgcgtgacgcagcagaggaaatccttgaactcgctgaaaaaacgggagcagccgttatgtgttccccccgtggtaaaggtatcttccctgaagatcatccccagtttgttggtgtcacaggtttaggcggtcatgcttccgtcttaacttatatggaacagcaacctccactacgcacactcgtattaggaacccgccttggtgaaccgacttctttctggagttcggcgttagttccaaaagaaggtttcatccatgtagatattgacccagaagtcccaggtgtagcctatccacaggttgaaactttcggggttcggtctgatatcaaagcttttgtggaagagttattggagcaattaccggatgctccccgttccacaactttgtcgctacctaatccagaacgcaaagcaatcgaacctgcaccagaggtagattatccagtgcggccagaagtattgatggcagcaattcaaaagataattgttgaaggtagcgatgcaatagtcatggcggagtgtggtaactcatttacttggtcaacgcatctactccaatttaccgaaaccaatcgttatcgagtcagcaccggagtcggcgcaatgggtcacgccgttacaggagtattgggtgcagcattggcaagcaataccaaggcggtaggcattgtaggcgatggggcaatgctgatgaataacgaaatcagcacagccgtgaaatacaaaattccggcgatttggattgtcctcaacgatgcacgttacaacatgtgccatcaagggatgaaaatcttgggattaaagggcgcagatgccacacttccaccgacaaacttcgccatgattgctcgtggcatgggagcagaagcgctcgtggttgttagagagtcggatattgaagcggcattagaacaagcgatcgcatcatctgttccctttctcattgatgtcgtaattgacgctgatcgcccagcaccttctggtggacgtaataagagtttggctgcacaaggaattaaatcaagtccagctaaaaatggagccaagcaagtttcatttccaatggtttgagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z