LOCUS BBa_K814015 5703 bp DNA linear 29-Nov-2024 DEFINITION G418 selection yeast shuttle vector designed for BioBrick ACCESSION BBa_K814015 VERSION BBa_K814015.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=BioBrick suffix stem_loop 21..91 /label=His operon terminator misc_feature 260..614 /label=rep (pBM1) stem_loop 926..1031 /label=T0 terminator CDS 1043..1703 /label=Chloramphenicol resistance misc_feature 1803..3009 /label=2u Yeast ORI stem_loop 3035..3259 /label=tTEF1 terminator CDS 3260..4069 /label=G418 resistance marker RBS 4070..4075 /label=Yeast Kozak promoter 4095..5539 /label=pADH1 primer_bind 155..170 /label=VR primer_bind 5565..5583 /label=VF2 stem_loop 5654..5673 /label=stem_loop ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt 61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct 121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 241 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga 301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctggtctga cgctcagtgg 901 aacgagccct taaccagcta gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa 961 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg 1021 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg 1081 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat 1141 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg 1201 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg 1261 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc 1321 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta 1381 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg 1441 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc 1501 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt 1561 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc 1621 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt 1681 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc 1741 aaaaaatacg cccggtagtg atctgggtcc ttatggtgaa agttggaccc tcctacgtgc 1801 cctaggaact cgctaccttt catcctacat aaatagacgc atataagtac gcatttaagc 1861 ataaacacgc actatgccgt tcttctcatg tatatatata tacaggcaac acgcagatat 1921 aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt cggaagcgct 1981 cgttttcgga aacgctttga agttcctatt ccgaagttcc tattcgctag aaagtatagg 2041 aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca aaaaaccaaa 2101 aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca caaacattgc 2161 tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc tacccatcca 2221 cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg tttatctcta 2281 gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga atcgaaaaca 2341 atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga agaaaccgtt 2401 cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca caaagtatgc 2461 gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa aatgcacccg 2521 cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt atggaagaga 2581 aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca aaaagttatc 2641 aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat gctttgttag 2701 aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag attctttgtt 2761 ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg 2821 tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag cacagattct 2881 tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc agctcagatt 2941 ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat gaagcacaga 3001 tgcttcgttc gttgggcatt gccctaagtt ggggtggatg gcggcgttag tatcgaatcg 3061 acagcagtat agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac 3121 ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat 3181 ttgattaaaa tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa 3241 caagaatctt tttattgtct tagaaaaact catcgagcat caaatgaaac tgcaatttat 3301 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 3361 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 3421 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 3481 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc 3541 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 3601 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 3661 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 3721 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttgccg gggatcgcag 3781 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 3841 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 3901 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 3961 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 4021 tgttggaatt taatcgcggc ctcgaaacgt gagtcttttc cttacccatt tttttggtct 4081 gctcaagcgg gcccagttga ttgtatgctt ggtatagctt gaaatattgt gcagaaaaag 4141 aaacaaggaa gaaagggaac gagaacaatg acgaggaaac aaaagattaa taattgcagg 4201 tctatttata cttgatagca aagcggcaaa ctttttttat ttcaaattca agtaactgga 4261 aggaaggccg tataccgttg ctcattagag agtagtgtgc gtgaatgaag gaaggaaaaa 4321 gtttcgtgtg ttcgaagata cccctcatca gctctggaac aacgacatct gttggtgctg 4381 tctttgtcgt taattttttc ctttagtgtc ttccatcatt ttttttgtca ttgcggatat 4441 ggtgagacaa caacggggga gagagaaaag aaaaaaaaag aaaagaagtt gcatgcgcct 4501 attattactt caatagatgg caaatggaaa aagggtagtg aaacttcgat atgatgatgg 4561 ctatcaagtc tagggctaca gtattagttc gttatgtacc accatcaatg aggcagtgta 4621 attggtgtag tcttgtttag cccattatgt cttgtctggt atctgttcta ttgtatatct 4681 cccctccgcc acctacatgt tagggagacc aacgaaggta ttataggaat cccgatgtat 4741 gggtttggtt gccagaaaag aggaagtcca tattgtacac ccggaaacaa caaaaggata 4801 tccgaaatat tccacggttt agaaaaaaat cggaaaagag cgcggagggg tgttaccccc 4861 cttctctact agcattggac tttaattaat atatgtgcat aggagaagtg taaagttccc 4921 ttccatattg taacataata aagtgcacac ccaaatgaat tgaaagcgta ctcaaacaga 4981 caaccatttc cagtgttgta tgtacctgtc tatttatact ggtagcaacc ctattgctgt 5041 ttcctcttca aagtactcta gcggttatgc gcgtctcacc ttcaaggtca tggtcgctct 5101 attgttcgca ccaccggcaa actcgcgtct cgcaagtctt ggctcattct tctagtatac 5161 tcatgttgca aatgcactca ggttctttcg gcaacttaaa taatgacacc agttgtcgtg 5221 gtcgtcatca tcgcaacccc aaccggcatt cttattgctt ctccaatctc gccccttagc 5281 gcagggtaaa ccttggaaaa tgcaggcgca aaaaactccg ccgggcacag cctcacgccc 5341 agcgttatcg ccgggccggc aagagcgcgg gtccgccaca gagtcagcat gattgtgcaa 5401 ttgcgtaaac tcgttttttc ggcgccgcaa agccaaatac atcatatcaa cacttttcac 5461 tttatttttc gttcgaccct tatatttgtc ttttgccttc atgctccttg atttcctatt 5521 tcatttacca tcatttcttg gggttccgcg cacatctcac aggggccacc tgacgtctaa 5581 gaaaccatta ttaccatgac attaacctat aaaaataggc gtatcacgag gcagaatttc 5641 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta 5701 gag //