##gff-version 3
##sequence-region BBa_K814015 1 5673
BBa_K814015	.	sequence_feature	1803	3009	.	+	0	ID=annotation2211686;Name=2u Yeast ORI
BBa_K814015	.	stem_loop	926	1031	.	+	0	ID=annotation2211684;Name=T0 terminator
BBa_K814015	.	stem_loop	21	91	.	+	0	ID=annotation2211682;Name=His operon terminator
BBa_K814015	.	stem_loop	5654	5673	.	+	0	ID=annotation2211693;Name=stem_loop
BBa_K814015	.	stem_loop	3035	3259	.	+	0	ID=annotation2211687;Name=tTEF1 terminator
BBa_K814015	.	primer_binding_site	155	170	.	+	0	ID=annotation2211691;Name=VR
BBa_K814015	.	CDS	1043	1703	.	+	0	ID=annotation2211685;Name=Chloramphenicol resistance
BBa_K814015	.	ribosome_entry_site	4070	4075	.	+	0	ID=annotation2211689;Name=Yeast Kozak
BBa_K814015	.	CDS	3260	4069	.	+	0	ID=annotation2211688;Name=G418 resistance marker
BBa_K814015	.	sequence_feature	1	20	.	+	0	ID=annotation2211681;Name=BioBrick suffix
BBa_K814015	.	primer_binding_site	5565	5583	.	+	0	ID=annotation2211692;Name=VF2
BBa_K814015	.	sequence_feature	260	614	.	+	0	ID=annotation2211683;Name=rep (pBM1)
BBa_K814015	.	promoter	4095	5539	.	+	0	ID=annotation2211690;Name=pADH1
>BBa_K814015
tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaaga
ttacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctc
actcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaa
ggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcga
cgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgct
ctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctc
acgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgac
cgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactg
gtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactag
aagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaa
caaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatc
ctttgatcttttctggtctgacgctcagtggaacgagcccttaaccagctagaggcttggattctcaccaataaaaaac
gcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaa
gcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacat
ggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcc
catggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattg
gctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcg
aatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaa
aacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagca
ttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccg
taatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgcca
ttgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataac
tcaaaaaatacgcccggtagtgatctgggtccttatggtgaaagttggaccctcctacgtgccctaggaactcgctacc
tttcatcctacataaatagacgcatataagtacgcatttaagcataaacacgcactatgccgttcttctcatgtatata
tatatacaggcaacacgcagatataggtgcgacgtgaacagtgagctgtatgtgcgcagctcgcgttgcattttcggaa
gcgctcgttttcggaaacgctttgaagttcctattccgaagttcctattcgctagaaagtataggaacttcagagcgct
tttgaaaaccaaaagcgctctgaagacgcactttcaaaaaaccaaaaacgcaccggactgtaacgagctactaaaatat
tgcgaataccgcttccacaaacattgctcaaaagtatctctttgctatatatctctgtgctatatccctatataaccta
cccatccacctttcgctccttgaacttgcatctaaactcgacctctacattttttatgtttatctctagtattactctt
tagacaaaaaaattgtagtaagaactattcatagagtgaatcgaaaacaatacgaaaatgtaaacatttcctatacgta
gtatatagagacaaaatagaagaaaccgttcataattttctgaccaatgaagaatcatcaacgctatcactttctgttc
acaaagtatgcgcaatccacatcggtatagaatataatcggggatgcctttatcttgaaaaaatgcacccgcagcttcg
ctagtaatcagtaaacgcgggaagtggagtcaggctttttttatggaagagaaaatagacaccaaagtagccttcttct
aaccttaacggacctacagtgcaaaaagttatcaagagactgcattatagagcgcacaaaggagaaaaaaagtaatcta
agatgctttgttagaaaaatagcgctctcgggatgcatttttgtagaacaaaaaagaagtatagattctttgttggtaa
aatagcgctctcgcgttgcatttctgttctgtaaaaatgcagctcagattctttgtttgaaaaattagcgctctcgcgt
tgcatttttgttttacaaaaatgaagcacagattcttcgttggtaaaatagcgctttcgcgttgcatttctgttctgta
aaaatgcagctcagattctttgtttgaaaaattagcgctctcgcgttgcatttttgttctacaaaatgaagcacagatg
cttcgttcgttgggcattgccctaagttggggtggatggcggcgttagtatcgaatcgacagcagtatagcgaccagca
ttcacatacgattgacgcatgatattactttctgcgcacttaacttcgcatctgggcagatgatgtcgaggcgaaaaaa
aatataaatcacgctaacatttgattaaaatagaacaactacaatataaaaaaactatacaaatgacaagttcttgaaa
acaagaatctttttattgtcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaa
taccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctg
gtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagt
gagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacag
gccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacg
aaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatca
acaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttgccggggatcgcagtggtgagtaacc
atgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccat
ctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaat
cgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaat
ttaatcgcggcctcgaaacgtgagtcttttccttacccatttttttggtctgctcaagcgggcccagttgattgtatgc
ttggtatagcttgaaatattgtgcagaaaaagaaacaaggaagaaagggaacgagaacaatgacgaggaaacaaaagat
taataattgcaggtctatttatacttgatagcaaagcggcaaactttttttatttcaaattcaagtaactggaaggaag
gccgtataccgttgctcattagagagtagtgtgcgtgaatgaaggaaggaaaaagtttcgtgtgttcgaagatacccct
catcagctctggaacaacgacatctgttggtgctgtctttgtcgttaattttttcctttagtgtcttccatcatttttt
ttgtcattgcggatatggtgagacaacaacgggggagagagaaaagaaaaaaaaagaaaagaagttgcatgcgcctatt
attacttcaatagatggcaaatggaaaaagggtagtgaaacttcgatatgatgatggctatcaagtctagggctacagt
attagttcgttatgtaccaccatcaatgaggcagtgtaattggtgtagtcttgtttagcccattatgtcttgtctggta
tctgttctattgtatatctcccctccgccacctacatgttagggagaccaacgaaggtattataggaatcccgatgtat
gggtttggttgccagaaaagaggaagtccatattgtacacccggaaacaacaaaaggatatccgaaatattccacggtt
tagaaaaaaatcggaaaagagcgcggaggggtgttaccccccttctctactagcattggactttaattaatatatgtgc
ataggagaagtgtaaagttcccttccatattgtaacataataaagtgcacacccaaatgaattgaaagcgtactcaaac
agacaaccatttccagtgttgtatgtacctgtctatttatactggtagcaaccctattgctgtttcctcttcaaagtac
tctagcggttatgcgcgtctcaccttcaaggtcatggtcgctctattgttcgcaccaccggcaaactcgcgtctcgcaa
gtcttggctcattcttctagtatactcatgttgcaaatgcactcaggttctttcggcaacttaaataatgacaccagtt
gtcgtggtcgtcatcatcgcaaccccaaccggcattcttattgcttctccaatctcgccccttagcgcagggtaaacct
tggaaaatgcaggcgcaaaaaactccgccgggcacagcctcacgcccagcgttatcgccgggccggcaagagcgcgggt
ccgccacagagtcagcatgattgtgcaattgcgtaaactcgttttttcggcgccgcaaagccaaatacatcatatcaac
acttttcactttatttttcgttcgacccttatatttgtcttttgccttcatgctccttgatttcctatttcatttacca
tcatttcttggggttccgcgcacatctcacaggggccacctgacgtctaagaaaccattattaccatgacattaaccta
taaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcg
cggccgcttctagag