BBa_K817017 1 BBa_K817017 pSB2K3 with parS site 2012-09-17T11:00:00Z 2015-05-08T01:13:28Z pseudomonas putida KT2440 chromosome pSB2K3 is mini F plasmid that lacks partition system and replicates as low copy in cell. pSB2K3 with parS site can be partitioned by parAB(BBa_K817007) cassette which can enhances its segregation stability. false false _1075_ 0 13960 9 It's complicated false parS site is put into biobricks cloning site not on backbone. false Shan Chi Hsieh annotation2187465 1 lacOP range2187465 1 2037 2211 annotation2187457 1 suffix range2187457 1 1 21 annotation2187469 1 VF2 range2187469 1 4286 4305 annotation2187473 1 parB binding site (parS) range2187473 1 4426 4441 annotation2187460 1 deletion of two bases range2187460 1 34 35 annotation2187471 1 terminator stem loop range2187471 1 4375 4394 annotation2187472 1 prefix range2187472 1 4404 4425 annotation2187463 1 repE (rev) range2187463 1 617 1372 annotation2187462 1 F1 Replication Origin range2187462 1 242 290 annotation2187458 1 E. coli his operon terminator range2187458 1 22 93 annotation2187470 1 end of terminator range2187470 1 4362 4365 annotation2187459 1 terminator stem loop range2187459 1 30 64 annotation2187466 1 repL range2187466 1 2289 3134 annotation2187468 1 kanR range2187468 1 3433 4248 annotation2187467 1 nnpp promoters range2187467 1 3242 3393 annotation2187461 1 VR range2187461 1 157 176 annotation2187464 1 oriS Replication Origin (rev) range2187464 1 1463 1679 BBa_K817017_sequence 1 tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatggcgcgccacgcgtacccataatacccataatagctgtttgccatcgcgtatgcatcgatcacgtgtccacgttctttaatagtggactcttgttccaaactggaacaacactcggatcgatccggcgcgcaccgtgggaaaaactccaggtagaggtacacacgcggatagccaattcagagtaataaactgtgataatcaaccctcatcaatgatgacgaactaacccccgatatcaggtcacatgacgaagggaaagagaaggaaatcaactgtgacaaactgccctcaaatttggcttccttaaaaattacagttcaaaaagtatgagaaaatccatgcaggctgaaggaaacagcaaaactgtgacaaattaccctcagtaggtcagaacaaatgtgacgaaccaccctcaaatctgtgacagataaccctcagactatcctgtcgtcatggaagtgatatcgcggaaggaaaatacgatatgagtcgtctggcggcctttctttttctcaatgtatgagaggcgcattggagttctgctgttgatctcattaacacagacttgcaggaagcggcggcggaagtcaggcatacgctggtaactttgaggcagctggtaacgctctatgatccagtcgattttcagagagacgatgcctgagccatccggcttacgatactgacacagggattcgtataaacgcatggcatacggattggtgatttcttttgtttcactaagccgaaactgcgtaaaccggttctgtaacccgataaagaagggaatgagatatgggttgatatgtacactgtaaagccctctggatggactgtgcgcacgtttgataaaccaaggaaaagattcatagcctttttcatcgccggcatcctcttcagggcgataaaaaaccacttccttccccgcgaaactcttcaatgcctgccgtatatccttactggcttccgcagaggtcaatccgaatatttcagcatatttagcaacatggatctcgcagataccgtcatgttcctgtagggtgccatcagattttctgatctggtcaacgaacagatacagcatacgtttttgatcccgggagagactatatgccgcctcagtgaggtcgtttgactggacgattcgcgggctatttttacgtttcttgtgattgataaccgctgtttccgccatgacagatccatgtgaagtgtgacaagtttttagattgtcacactaaataaaaaagagtcaataagcagggataactttgtgaaaaaacagcttcttctgagggcaatttgtcacagggttaagggcaatttgtcacagacaggactgtcatttgagggtgatttgtcacactgaaagggcaatttgtcacaacaccttctgtagaaccagcatggataaaggcctacaaggcgctctaaaaaagaagatctaaaaactataaaaaaaataattataaaaatatccccgtggataagtggataaccccaagggaagttttttcaggcatcgtgtgtaagcagaatatataagtgctgttccctggtgcttcctcgctcactcgaaattcccggggatagctttatgcttgtaaaccgttttgtgaaaaaatttttaaaataaaaaaggggacctctagggtccccaattaattagtaatataatctattaaaggtcattcaaaaggtcatccaccggatcaattcccctgctcgcgcaggctgggtgccaagctctcgggtaacatcaaggcccgatccttggagcccttgccctcccgcacgatgatcgtgccgtgatcgaaatccagatccttgacccgcagttgcaaaccctcactgatccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcgcggtacctgtcgcggcaacgcgctaacagacgtagtaagaaccaccagcattgtaatgctggctaaagtcactttcctgagctgtataacgatgagcgattttactttttctggctatgaattggcctgctttgtaacacactccggtctatcccgtagcgccgggcatatcctgtcgcaatgtgcaaatctcgcggcaacaaccagtgaatacttcattcacaagcctcaccgcctgatcgcggcagaaactggttatagccaatcaaccgtcgttcgtgcattccgtgaagctgtaaacaaaggtatcctgtctgtagagattgttatcggcgatcaccgtgaacgtcgcgctaacctgtaccggtttacaccatcctttttggccttcgcacaacaagccaaaaatgcgctgatagaaagcaaattaaagatctcttcagcggcaaccaaggttaaagctgttctcgctaagacattggctttatttaattttttatccacacccccatgtcaaaatgataccccctccccctgtcaggatgacgtggcaataaagaataagaagtcacaagttaaaaaaacaaaaagatcagtttccggcggtgccggaacaaccagcctcaaaaaattgacttcatggatcgctaaggcaaaagcaaaggctgacaatctgcggttatccaaaaaacgcactcaaaaacatgagttcaagcagaaagtagaggcggctgcgcggaaatatgcttacctgaagaacaagcgttcgcctgatattggcgggatatcaaacttcgataacctaccgcattgcatgacggtaaacgaagctcttaatgcggttttagccaaaaataaagataacgaacaatggggtataccggcaggattcagagggtaatgaattgctctaattataaccatgcatactttcaacacctctagtttgccatgaggcaaactcataggtgtcctggtaagaggacactgttgccaaaactggacgccccattattgcaattaataaacaactaacggacaattctacctaacaataagtggagttgcggccggccgcgatccgctagcaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaactgttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcgagctctgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagggttccacgtggaaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z