##gff-version 3
##sequence-region BBa_K817017 1 4441
BBa_K817017	.	promoter	3242	3393	.	+	0	ID=annotation2187467;Name=nnpp promoters
BBa_K817017	.	stem_loop	30	64	.	+	0	ID=annotation2187459;Name=terminator stem loop
BBa_K817017	.	engineered_region	1	21	.	+	0	ID=annotation2187457;Name=suffix
BBa_K817017	.	promoter	242	290	.	+	0	ID=annotation2187462;Name=F1 Replication Origin
BBa_K817017	.	operator	2037	2211	.	+	0	ID=annotation2187465;Name=lacOP
BBa_K817017	.	primer_binding_site	4286	4305	.	+	0	ID=annotation2187469;Name=VF2
BBa_K817017	.	CDS	3433	4248	.	+	0	ID=annotation2187468;Name=kanR
BBa_K817017	.	CDS	617	1372	.	+	0	ID=annotation2187463;Name=repE (rev)
BBa_K817017	.	stem_loop	4375	4394	.	+	0	ID=annotation2187471;Name=terminator stem loop
BBa_K817017	.	sequence_feature	22	93	.	+	0	ID=annotation2187458;Name=E. coli his operon terminator
BBa_K817017	.	sequence_feature	4362	4365	.	+	0	ID=annotation2187470;Name=end of terminator
BBa_K817017	.	engineered_region	4404	4425	.	+	0	ID=annotation2187472;Name=prefix
BBa_K817017	.	CDS	2289	3134	.	+	0	ID=annotation2187466;Name=repL
BBa_K817017	.	sequence_feature	34	35	.	+	0	ID=annotation2187460;Name=deletion of two bases
BBa_K817017	.	primer_binding_site	157	176	.	+	0	ID=annotation2187461;Name=VR
BBa_K817017	.	promoter	1463	1679	.	+	0	ID=annotation2187464;Name=oriS Replication Origin (rev)
BBa_K817017	.	non_covalent_binding_site	4426	4441	.	+	0	ID=annotation2187473;Name=parB binding site (parS)
>BBa_K817017
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaatggcgcgccacgcgtacccataatacccataatagctgtttgccatcgcgtatgcatcgatc
acgtgtccacgttctttaatagtggactcttgttccaaactggaacaacactcggatcgatccggcgcgcaccgtggga
aaaactccaggtagaggtacacacgcggatagccaattcagagtaataaactgtgataatcaaccctcatcaatgatga
cgaactaacccccgatatcaggtcacatgacgaagggaaagagaaggaaatcaactgtgacaaactgccctcaaatttg
gcttccttaaaaattacagttcaaaaagtatgagaaaatccatgcaggctgaaggaaacagcaaaactgtgacaaatta
ccctcagtaggtcagaacaaatgtgacgaaccaccctcaaatctgtgacagataaccctcagactatcctgtcgtcatg
gaagtgatatcgcggaaggaaaatacgatatgagtcgtctggcggcctttctttttctcaatgtatgagaggcgcattg
gagttctgctgttgatctcattaacacagacttgcaggaagcggcggcggaagtcaggcatacgctggtaactttgagg
cagctggtaacgctctatgatccagtcgattttcagagagacgatgcctgagccatccggcttacgatactgacacagg
gattcgtataaacgcatggcatacggattggtgatttcttttgtttcactaagccgaaactgcgtaaaccggttctgta
acccgataaagaagggaatgagatatgggttgatatgtacactgtaaagccctctggatggactgtgcgcacgtttgat
aaaccaaggaaaagattcatagcctttttcatcgccggcatcctcttcagggcgataaaaaaccacttccttccccgcg
aaactcttcaatgcctgccgtatatccttactggcttccgcagaggtcaatccgaatatttcagcatatttagcaacat
ggatctcgcagataccgtcatgttcctgtagggtgccatcagattttctgatctggtcaacgaacagatacagcatacg
tttttgatcccgggagagactatatgccgcctcagtgaggtcgtttgactggacgattcgcgggctatttttacgtttc
ttgtgattgataaccgctgtttccgccatgacagatccatgtgaagtgtgacaagtttttagattgtcacactaaataa
aaaagagtcaataagcagggataactttgtgaaaaaacagcttcttctgagggcaatttgtcacagggttaagggcaat
ttgtcacagacaggactgtcatttgagggtgatttgtcacactgaaagggcaatttgtcacaacaccttctgtagaacc
agcatggataaaggcctacaaggcgctctaaaaaagaagatctaaaaactataaaaaaaataattataaaaatatcccc
gtggataagtggataaccccaagggaagttttttcaggcatcgtgtgtaagcagaatatataagtgctgttccctggtg
cttcctcgctcactcgaaattcccggggatagctttatgcttgtaaaccgttttgtgaaaaaatttttaaaataaaaaa
ggggacctctagggtccccaattaattagtaatataatctattaaaggtcattcaaaaggtcatccaccggatcaattc
ccctgctcgcgcaggctgggtgccaagctctcgggtaacatcaaggcccgatccttggagcccttgccctcccgcacga
tgatcgtgccgtgatcgaaatccagatccttgacccgcagttgcaaaccctcactgatccgattcattaatgcagctgg
cacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacccc
aggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagcta
tgaccatgattacgccaagcgcggtacctgtcgcggcaacgcgctaacagacgtagtaagaaccaccagcattgtaatg
ctggctaaagtcactttcctgagctgtataacgatgagcgattttactttttctggctatgaattggcctgctttgtaa
cacactccggtctatcccgtagcgccgggcatatcctgtcgcaatgtgcaaatctcgcggcaacaaccagtgaatactt
cattcacaagcctcaccgcctgatcgcggcagaaactggttatagccaatcaaccgtcgttcgtgcattccgtgaagct
gtaaacaaaggtatcctgtctgtagagattgttatcggcgatcaccgtgaacgtcgcgctaacctgtaccggtttacac
catcctttttggccttcgcacaacaagccaaaaatgcgctgatagaaagcaaattaaagatctcttcagcggcaaccaa
ggttaaagctgttctcgctaagacattggctttatttaattttttatccacacccccatgtcaaaatgataccccctcc
ccctgtcaggatgacgtggcaataaagaataagaagtcacaagttaaaaaaacaaaaagatcagtttccggcggtgccg
gaacaaccagcctcaaaaaattgacttcatggatcgctaaggcaaaagcaaaggctgacaatctgcggttatccaaaaa
acgcactcaaaaacatgagttcaagcagaaagtagaggcggctgcgcggaaatatgcttacctgaagaacaagcgttcg
cctgatattggcgggatatcaaacttcgataacctaccgcattgcatgacggtaaacgaagctcttaatgcggttttag
ccaaaaataaagataacgaacaatggggtataccggcaggattcagagggtaatgaattgctctaattataaccatgca
tactttcaacacctctagtttgccatgaggcaaactcataggtgtcctggtaagaggacactgttgccaaaactggacg
ccccattattgcaattaataaacaactaacggacaattctacctaacaataagtggagttgcggccggccgcgatccgc
tagcaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaac
tgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgatt
aaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctat
cgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatg
agatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgc
atggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatatt
gttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtat
ttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctg
gcctgttgaacaagtctggaaagaaatgcataaactgttgccattctcaccggattcagtcgtcactcatggtgatttc
tcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgat
accaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatgg
tattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattgg
ttgtaacactggcgagctctgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgta
tcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctaga
gggttccacgtggaaca