LOCUS BBa_K817031 3430 bp DNA linear 16-Jun-2024 DEFINITION PfadBA-TetR(P0340)-pLac ACCESSION BBa_K817031 VERSION BBa_K817031.1 FEATURES Location/Qualifiers promoter 1..72 /label=PfadBA promoter 1..72 /label=BBa_K817002 misc_feature 87..90 /label=conserved RBS 81..94 /label=BBa_B0031 CDS 104..720 /label=tetR misc_feature 721..754 /label=SsrA misc_feature 761..785 /label=Help:Barcodes CDS 101..760 /label=BBa_C0040 stem_loop 805..848 /label=stem_loop misc_feature 794..873 /label=BBa_B0010 terminator 794..873 /label=BBa_B0010 stem_loop 889..908 /label=T7 TE misc_feature 915..915 /label=stop polyA_site 909..922 /label=polya misc_feature 882..922 /label=BBa_B0012 terminator 882..922 /label=BBa_B0012 misc_feature 81..922 /label=BBa_P0140 misc_feature 931..949 /label=TetR 1 misc_feature 931..984 /label=BBa_R0040 promoter 950..955 /label=-35 misc_feature 956..974 /label=TetR 2 promoter 973..978 /label=-10 promoter 931..984 /label=BBa_R0040 misc_feature 997..1000 /label=conserved RBS 993..1004 /label=BBa_B0034 misc_feature 2100..2138 /label=LVA CDS 1011..2138 /label=lacI-LVA misc_feature 1011..2138 /label=BBa_C0012 misc_feature 2139..2163 /label=Help:Barcodes CDS 1011..2138 /label=BBa_C0012 misc_feature 993..2163 /label=BBa_S0100 misc_feature 931..2163 /label=BBa_I763025 stem_loop 2183..2226 /label=stem_loop misc_feature 2172..2251 /label=BBa_B0010 terminator 2172..2251 /label=BBa_B0010 stem_loop 2267..2286 /label=T7 TE misc_feature 2293..2293 /label=stop polyA_site 2287..2300 /label=polya misc_feature 2260..2300 /label=BBa_B0012 terminator 2260..2300 /label=BBa_B0012 terminator 2172..2300 /label=BBa_B0015 misc_feature 931..2300 /label=BBa_I763026 CDS 2309..2396 /label=end of LacI coding region (inactive) misc_feature 2309..2508 /label=BBa_R0010 misc_feature 2397..2434 /label=CAP binding site promoter 2445..2450 /label=-35 promoter 2469..2474 /label=-10 misc_feature 2474..2508 /label=LacI binding site misc_feature 2481..2481 /label=start promoter 2309..2508 /label=BBa_R0010 misc_feature 2521..2524 /label=conserved RBS 2517..2528 /label=BBa_B0034 misc_feature 2535..3254 /label=BBa_E0040 misc_feature 3249..3287 /label=LVA misc_feature 3288..3293 /label=Double Stop misc_feature 2535..2537 /label=Start misc_feature 2535..3293 /label=BBa_J04031 stem_loop 3313..3356 /label=stem_loop misc_feature 3302..3381 /label=BBa_B0010 terminator 3302..3381 /label=BBa_B0010 stem_loop 3397..3416 /label=T7 TE misc_feature 3423..3423 /label=stop polyA_site 3417..3430 /label=polya misc_feature 3390..3430 /label=BBa_B0012 terminator 3390..3430 /label=BBa_B0012 misc_feature 2309..3430 /label=BBa_I763004 misc_feature 931..3430 /label=BBa_I763027 ORIGIN 1 atctggtacg accagatttg acaatctggt acgaccagat gatactgagc acatcagcag 61 gacgcactga cctactagag tcacacagga aacctactag atgtccagat tagataaaag 121 taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac 181 ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa 241 taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt 301 ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag 361 atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga 421 aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact 481 agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga 541 agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc 601 gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt 661 attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc 721 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag 781 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 841 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 901 ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct 961 atcagtgata gagatactga gcactactag agaaagagga gaaatactag atggtgaatg 1021 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt 1081 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg 1141 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt 1201 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg 1261 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac 1321 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg 1381 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca 1441 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt 1501 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc 1561 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct 1621 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga 1681 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg 1741 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg 1801 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat 1861 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg 1921 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg 1981 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg 2041 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg 2101 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat 2161 ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 2221 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 2281 tgggcctttc tgcgtttata tactagagca atacgcaaac cgcctctccc cgcgcgttgg 2341 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 2401 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 2461 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagaaag 2521 aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg 2581 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg 2641 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc 2701 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag 2761 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa 2821 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag 2881 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca 2941 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca 3001 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg 3061 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac 3121 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag 3181 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac 3241 tatacaaaag gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga 3301 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 3361 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 3421 tgcgtttata //