##gff-version 3
##sequence-region BBa_K817031 1 3430
BBa_K817031	.	engineered_region	81	922	.	+	0	ID=BBa_P0140;Name=BBa_P0140
BBa_K817031	.	terminator	794	873	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0140
BBa_K817031	.	stem_loop	805	848	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K817031	.	engineered_region	794	873	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K817031	.	terminator	882	922	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0140
BBa_K817031	.	engineered_region	882	922	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K817031	.	stop_codon	915	915	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K817031	.	stem_loop	889	908	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K817031	.	polyA_site	909	922	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K817031	.	ribosome_entry_site	81	94	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0140
BBa_K817031	.	conserved_region	87	90	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K817031	.	CDS	101	760	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0140
BBa_K817031	.	engineered_tag	761	785	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K817031	.	tag	721	754	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K817031	.	CDS	104	720	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K817031	.	promoter	1	72	.	+	0	ID=BBa_K817002;Name=BBa_K817002
BBa_K817031	.	promoter	1	72	.	+	0	ID=annotation2197686;Name=PfadBA;Parent=BBa_K817002
BBa_K817031	.	engineered_region	931	3430	.	+	0	ID=BBa_I763027;Name=BBa_I763027
BBa_K817031	.	engineered_region	2309	3430	.	+	0	ID=BBa_I763004;Name=BBa_I763004;Parent=BBa_I763027
BBa_K817031	.	promoter	2309	2508	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_I763004
BBa_K817031	.	non_covalent_binding_site	2474	2508	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K817031	.	non_covalent_binding_site	2397	2434	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K817031	.	engineered_region	2309	2508	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K817031	.	promoter	2445	2450	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K817031	.	CDS	2309	2396	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K817031	.	start_codon	2481	2481	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K817031	.	promoter	2469	2474	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K817031	.	terminator	3302	3381	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763004
BBa_K817031	.	stem_loop	3313	3356	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K817031	.	engineered_region	3302	3381	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K817031	.	ribosome_entry_site	2517	2528	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763004
BBa_K817031	.	conserved_region	2521	2524	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K817031	.	engineered_region	2535	3293	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763004
BBa_K817031	.	engineered_region	2535	3254	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_K817031	.	tag	3249	3287	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_K817031	.	stop_codon	3288	3293	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_K817031	.	start_codon	2535	2537	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_K817031	.	terminator	3390	3430	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763004
BBa_K817031	.	engineered_region	3390	3430	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K817031	.	stop_codon	3423	3423	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K817031	.	stem_loop	3397	3416	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K817031	.	polyA_site	3417	3430	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K817031	.	engineered_region	931	2300	.	+	0	ID=BBa_I763026;Name=BBa_I763026;Parent=BBa_I763027
BBa_K817031	.	engineered_region	931	2163	.	+	0	ID=BBa_I763025;Name=BBa_I763025;Parent=BBa_I763026
BBa_K817031	.	engineered_region	993	2163	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_I763025
BBa_K817031	.	CDS	1011	2138	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_K817031	.	engineered_tag	2139	2163	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K817031	.	CDS	1011	2138	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K817031	.	tag	2100	2138	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K817031	.	engineered_region	1011	2138	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K817031	.	ribosome_entry_site	993	1004	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_K817031	.	conserved_region	997	1000	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K817031	.	promoter	931	984	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I763025
BBa_K817031	.	promoter	950	955	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K817031	.	non_covalent_binding_site	956	974	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K817031	.	engineered_region	931	984	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K817031	.	non_covalent_binding_site	931	949	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K817031	.	promoter	973	978	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K817031	.	terminator	2172	2300	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I763026
BBa_K817031	.	terminator	2172	2251	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K817031	.	stem_loop	2183	2226	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K817031	.	engineered_region	2172	2251	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K817031	.	terminator	2260	2300	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K817031	.	engineered_region	2260	2300	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K817031	.	stop_codon	2293	2293	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K817031	.	stem_loop	2267	2286	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K817031	.	polyA_site	2287	2300	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K817031
atctggtacgaccagatttgacaatctggtacgaccagatgatactgagcacatcagcaggacgcactgacctactaga
gtcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgagg
tcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaa
aaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggg
gaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaag
tacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttt
ttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcat
caagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttg
atcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtga
aagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagag
attgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaacc
agtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccac
gtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaac
tggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggc
gattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaa
gcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattg
ctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattatttt
ctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggc
ccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatag
cggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccac
tgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcg
gatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggatt
ttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgtt
gcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattca
ttaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctt
aataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacagg
tttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttac
actttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
tactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggc
acaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgg
aaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcat
atgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacg
ggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattt
taaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaa
caaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatc
aacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaa
agatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaa
ctatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata