##gff-version 3
##sequence-region BBa_K817032 1 3427
BBa_K817032	.	promoter	1	72	.	+	0	ID=BBa_K817002;Name=BBa_K817002
BBa_K817032	.	promoter	1	72	.	+	0	ID=annotation2197686;Name=PfadBA;Parent=BBa_K817002
BBa_K817032	.	engineered_region	928	3427	.	+	0	ID=BBa_I763027;Name=BBa_I763027
BBa_K817032	.	engineered_region	928	2297	.	+	0	ID=BBa_I763026;Name=BBa_I763026;Parent=BBa_I763027
BBa_K817032	.	terminator	2169	2297	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I763026
BBa_K817032	.	terminator	2169	2248	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K817032	.	stem_loop	2180	2223	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K817032	.	engineered_region	2169	2248	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K817032	.	terminator	2257	2297	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K817032	.	stop_codon	2290	2290	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K817032	.	engineered_region	2257	2297	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K817032	.	stem_loop	2264	2283	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K817032	.	polyA_site	2284	2297	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K817032	.	engineered_region	928	2160	.	+	0	ID=BBa_I763025;Name=BBa_I763025;Parent=BBa_I763026
BBa_K817032	.	engineered_region	990	2160	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_I763025
BBa_K817032	.	CDS	1008	2135	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_K817032	.	engineered_region	1008	2135	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K817032	.	engineered_tag	2136	2160	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K817032	.	CDS	1008	2135	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K817032	.	tag	2097	2135	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K817032	.	ribosome_entry_site	990	1001	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_K817032	.	conserved_region	994	997	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K817032	.	promoter	928	981	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I763025
BBa_K817032	.	promoter	947	952	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K817032	.	non_covalent_binding_site	953	971	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K817032	.	engineered_region	928	981	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K817032	.	non_covalent_binding_site	928	946	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K817032	.	promoter	970	975	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K817032	.	engineered_region	2306	3427	.	+	0	ID=BBa_I763004;Name=BBa_I763004;Parent=BBa_I763027
BBa_K817032	.	terminator	3299	3378	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763004
BBa_K817032	.	stem_loop	3310	3353	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K817032	.	engineered_region	3299	3378	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K817032	.	engineered_region	2532	3290	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763004
BBa_K817032	.	tag	3246	3284	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_K817032	.	start_codon	2532	2534	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_K817032	.	engineered_region	2532	3251	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_K817032	.	stop_codon	3285	3290	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_K817032	.	ribosome_entry_site	2514	2525	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763004
BBa_K817032	.	conserved_region	2518	2521	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K817032	.	promoter	2306	2505	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_I763004
BBa_K817032	.	non_covalent_binding_site	2394	2431	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K817032	.	promoter	2442	2447	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K817032	.	engineered_region	2306	2505	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K817032	.	CDS	2306	2393	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K817032	.	promoter	2466	2471	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K817032	.	start_codon	2478	2478	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K817032	.	non_covalent_binding_site	2471	2505	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K817032	.	terminator	3387	3427	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763004
BBa_K817032	.	stop_codon	3420	3420	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K817032	.	engineered_region	3387	3427	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K817032	.	stem_loop	3394	3413	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K817032	.	polyA_site	3414	3427	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K817032	.	engineered_region	81	919	.	+	0	ID=BBa_P0340;Name=BBa_P0340
BBa_K817032	.	CDS	98	757	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0340
BBa_K817032	.	engineered_tag	758	782	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K817032	.	tag	718	751	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K817032	.	CDS	101	717	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K817032	.	terminator	879	919	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0340
BBa_K817032	.	stop_codon	912	912	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K817032	.	engineered_region	879	919	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K817032	.	stem_loop	886	905	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K817032	.	polyA_site	906	919	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K817032	.	terminator	791	870	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0340
BBa_K817032	.	stem_loop	802	845	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K817032	.	engineered_region	791	870	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K817032	.	ribosome_entry_site	81	91	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0340
BBa_K817032	.	ribosome_entry_site	87	90	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K817032	.	ribosome_entry_site	81	91	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K817032	.	engineered_region	81	91	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
>BBa_K817032
atctggtacgaccagatttgacaatctggtacgaccagatgatactgagcacatcagcaggacgcactgacctactaga
gtcacacaggactactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagatt
gacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagt
aacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtt
tctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactgg
cgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgat
taaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcg
gcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctg
tggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctc
ccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggccca
ttaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcgg
aacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgc
gatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggat
atctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttc
gcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcc
cgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcatta
atgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaat
aactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggttt
cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacact
ttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatac
tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata