BBa_K817034
1
BBa_K817034
J23119-RBS-SP1-Penetratin-Flag-xx
2012-09-23T11:00:00Z
2015-05-08T01:13:28Z
J23119-RBS-SP1-Penetratin-Flag-xx
J23119-RBS-SP1-Penetratin-Flag-xx
false
false
_1075_
0
13572
9
It's complicated
false
J23119-RBS-SP1-Penetratin-Flag-xx
false
Yen-Der Li
component2201615
1
BBa_J23119
component2201631
1
BBa_K817024
annotation2201631
1
BBa_K817024
range2201631
1
44
342
annotation2201615
1
BBa_J23119
range2201615
1
1
35
BBa_K817027
1
BBa_K817027
SP1-Penetratin-Flag
2012-09-22T11:00:00Z
2015-05-08T01:13:28Z
SP1-Penetratin-Flag
SP1-Penetratin-Flag
false
false
_1075_
0
13572
9
Not in stock
false
SP1-Penetratin-Flag
false
Jui-Tsen Yu
annotation2201516
1
FLAG
range2201516
1
121
141
annotation2201466
1
SP1
range2201466
1
1
63
annotation2201513
1
CPP
range2201513
1
64
120
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K817021
1
BBa_K817021
RBS-SP1-Penetratin-Flag
2012-09-17T11:00:00Z
2015-05-08T01:13:28Z
RBS-SP1-xx
Released HQ 2013
RBS-SP1-xx
false
false
_1075_
0
13572
9
In stock
false
RBS-SP1-xx
false
Jui-Tsen Yu
component2201523
1
BBa_K817027
component2201518
1
BBa_B0030
annotation2201523
1
BBa_K817027
range2201523
1
22
162
annotation2201518
1
BBa_B0030
range2201518
1
1
15
BBa_K817024
1
BBa_K817024
RBS-SP1-Penetratin-Flag-xx
2012-09-17T11:00:00Z
2015-05-08T01:13:28Z
SP1-xx
Released HQ 2013
SP1-xx
false
false
_1075_
0
13572
9
In stock
false
SP1-xx
false
Jui-Tsen Yu
component2201577
1
BBa_B0015
component2201570
1
BBa_K817021
annotation2201577
1
BBa_B0015
range2201577
1
171
299
annotation2201570
1
BBa_K817021
range2201570
1
1
162
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K817021_sequence
1
attaaagaggagaaatactagatgaaacagagcaccattgcgctggcgctgctgccgctgctgtttaccccggtgaccaaagcgcgccagattaaaatttggtttcagaaccgccgcatgaaatggaaaaaagattataaagatgatgatgataaataataa
BBa_K817027_sequence
1
atgaaacagagcaccattgcgctggcgctgctgccgctgctgtttaccccggtgaccaaagcgcgccagattaaaatttggtttcagaaccgccgcatgaaatggaaaaaagattataaagatgatgatgataaataataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K817034_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgaaacagagcaccattgcgctggcgctgctgccgctgctgtttaccccggtgaccaaagcgcgccagattaaaatttggtttcagaaccgccgcatgaaatggaaaaaagattataaagatgatgatgataaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_K817024_sequence
1
attaaagaggagaaatactagatgaaacagagcaccattgcgctggcgctgctgccgctgctgtttaccccggtgaccaaagcgcgccagattaaaatttggtttcagaaccgccgcatgaaatggaaaaaagattataaagatgatgatgataaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z