LOCUS       BBa_K817060             1491 bp    DNA     linear       29-Jun-2024
DEFINITION  PfadBA-RBS-GLP1-xx-Plac-RBS-fadR-xx
ACCESSION   BBa_K817060
VERSION     BBa_K817060.1  
FEATURES             Location/Qualifiers
     promoter        1..72
                     /label=PfadBA
     promoter        1..72
                     /label=BBa_K817002
     RBS             81..95
                     /label=RBS-1\Strong
     RBS             88..92
                     /label=RBS
     misc_feature    81..95
                     /label=BBa_B0030
     RBS             81..95
                     /label=BBa_B0030
     misc_feature    102..165
                     /label=SP1
     CDS             166..263
                     /label=GLP-1
     CDS             102..263
                     /label=BBa_K817000
     stem_loop       283..326
                     /label=stem_loop
     misc_feature    272..351
                     /label=BBa_B0010
     terminator      272..351
                     /label=BBa_B0010
     stem_loop       367..386
                     /label=T7 TE
     misc_feature    393..393
                     /label=stop
     polyA_site      387..400
                     /label=polya
     misc_feature    360..400
                     /label=BBa_B0012
     terminator      360..400
                     /label=BBa_B0012
     CDS             409..496
                     /label=end of LacI coding region (inactive)
     misc_feature    409..608
                     /label=BBa_R0010
     misc_feature    497..534
                     /label=CAP binding site
     promoter        545..550
                     /label=-35
     promoter        569..574
                     /label=-10
     misc_feature    574..608
                     /label=LacI binding site
     misc_feature    581..581
                     /label=start
     promoter        409..608
                     /label=BBa_R0010
     misc_feature    621..624
                     /label=conserved
     RBS             617..628
                     /label=BBa_B0034
     misc_feature    409..628
                     /label=BBa_J04500
     CDS             635..1354
                     /label=FadR
     CDS             635..1354
                     /label=BBa_K817001
     misc_feature    409..1354
                     /label=BBa_K817007
     stem_loop       1374..1417
                     /label=stem_loop
     misc_feature    1363..1442
                     /label=BBa_B0010
     terminator      1363..1442
                     /label=BBa_B0010
     stem_loop       1458..1477
                     /label=T7 TE
     misc_feature    1484..1484
                     /label=stop
     polyA_site      1478..1491
                     /label=polya
     misc_feature    1451..1491
                     /label=BBa_B0012
     terminator      1451..1491
                     /label=BBa_B0012
ORIGIN
        1 atctggtacg accagatttg acaatctggt acgaccagat gatactgagc acatcagcag
       61 gacgcactga cctactagag attaaagagg agaaatacta gatgaaacag agcaccattg
      121 cgctggcgct gctgccgctg ctgtttaccc cggtgaccaa agcgcatgcg gaaggcacct
      181 ttaccagcga tgtgagcagc tatctggaag gccaggcggc gaaagaattt attgcgtggc
      241 tggtgaaagg ccgcggctaa taatactaga gccaggcatc aaataaaacg aaaggctcag
      301 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      361 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagca atacgcaaac
      421 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
      481 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      541 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      601 ttcacacata ctagagaaag aggagaaata ctagatggtc attaaggcgc aaagcccggc
      661 gggtttcgcg gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac
      721 tattttgccc gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg
      781 tgaagtgtta cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac
      841 gaaggtgaat aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact
      901 ggatcacgaa agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc
      961 cactattttt attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc
     1021 taccgctaat gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt
     1081 ccgcggcctg gcgtttgctt ccggcaaccc gatttacggt ctgattctta acgggatgaa
     1141 agggctgtat acgcgtattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc
     1201 gctgggcttc taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta
     1261 cgaaacagtg cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa
     1321 tctgccgggt gatttagcca ttcaggggcg ataatactag agccaggcat caaataaaac
     1381 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1441 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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