##gff-version 3
##sequence-region BBa_K817060 1 1491
BBa_K817060	.	CDS	102	263	.	+	0	ID=BBa_K817000;Name=BBa_K817000
BBa_K817060	.	CDS	166	263	.	+	0	ID=annotation2201480;Name=GLP-1;Parent=BBa_K817000
BBa_K817060	.	tag	102	165	.	+	0	ID=annotation2201479;Name=SP1;Parent=BBa_K817000
BBa_K817060	.	terminator	1363	1442	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K817060	.	stem_loop	1374	1417	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K817060	.	engineered_region	1363	1442	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K817060	.	terminator	360	400	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K817060	.	stem_loop	367	386	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K817060	.	stop_codon	393	393	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K817060	.	engineered_region	360	400	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K817060	.	polyA_site	387	400	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K817060	.	terminator	272	351	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K817060	.	stem_loop	283	326	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K817060	.	engineered_region	272	351	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K817060	.	engineered_region	409	1354	.	+	0	ID=BBa_K817007;Name=BBa_K817007
BBa_K817060	.	engineered_region	409	628	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K817007
BBa_K817060	.	promoter	409	608	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K817060	.	engineered_region	409	608	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K817060	.	promoter	569	574	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K817060	.	CDS	409	496	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K817060	.	non_covalent_binding_site	497	534	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K817060	.	start_codon	581	581	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K817060	.	non_covalent_binding_site	574	608	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K817060	.	promoter	545	550	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K817060	.	ribosome_entry_site	617	628	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K817060	.	conserved_region	621	624	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K817060	.	CDS	635	1354	.	+	0	ID=BBa_K817001;Name=BBa_K817001;Parent=BBa_K817007
BBa_K817060	.	CDS	635	1354	.	+	0	ID=annotation2187738;Name=FadR;Parent=BBa_K817001
BBa_K817060	.	terminator	1451	1491	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K817060	.	stem_loop	1458	1477	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K817060	.	stop_codon	1484	1484	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K817060	.	engineered_region	1451	1491	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K817060	.	polyA_site	1478	1491	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K817060	.	ribosome_entry_site	81	95	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K817060	.	ribosome_entry_site	88	92	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K817060	.	engineered_region	81	95	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K817060	.	ribosome_entry_site	81	95	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K817060	.	promoter	1	72	.	+	0	ID=BBa_K817002;Name=BBa_K817002
BBa_K817060	.	promoter	1	72	.	+	0	ID=annotation2197686;Name=PfadBA;Parent=BBa_K817002
>BBa_K817060
atctggtacgaccagatttgacaatctggtacgaccagatgatactgagcacatcagcaggacgcactgacctactaga
gattaaagaggagaaatactagatgaaacagagcaccattgcgctggcgctgctgccgctgctgtttaccccggtgacc
aaagcgcatgcggaaggcacctttaccagcgatgtgagcagctatctggaaggccaggcggcgaaagaatttattgcgt
ggctggtgaaaggccgcggctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttc
ccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactt
tatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatact
agatggtcattaaggcgcaaagcccggcgggtttcgcggaagagtacattattgaaagtatctggaataaccgcttccc
tcccgggactattttgcccgcagaacgtgaactttcagaattaattggcgtaacgcgtactacgttacgtgaagtgtta
cagcgtctggcacgagatggctggttgaccattcaacatggcaagccgacgaaggtgaataatttctgggaaacttccg
gtttaaatatccttgaaacactggcgcgactggatcacgaaagtgtgccgcagcttattgataatttgctgtcggtgcg
taccaatatttccactatttttattcgcaccgcgtttcgtcagcatcccgataaagcgcaggaagtgctggctaccgct
aatgaagtggccgatcacgccgatgcctttgccgagctggattacaacatattccgcggcctggcgtttgcttccggca
acccgatttacggtctgattcttaacgggatgaaagggctgtatacgcgtattggtcgtcactatttcgccaatccgga
agcgcgcagtctggcgctgggcttctaccacaaactgtcggcgttgtgcagtgaaggcgcgcacgatcaggtgtacgaa
acagtgcgtcgctatgggcatgagagtggcgagatttggcaccggatgcagaaaaatctgccgggtgatttagccattc
aggggcgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata