LOCUS BBa_K818000 5223 bp DNA linear 22-Nov-2024 DEFINITION Integration vector for Bacillus subtilis derived from pSac-Cm ACCESSION BBa_K818000 VERSION BBa_K818000.1 FEATURES Location/Qualifiers misc_feature 1..17 /label=BioBrick suffix misc_feature 18..170 /label=BBa_B0015 CDS 181..990 /label='sacA CDS 1324..2196 /label=bla CDS 3187..3930 /label=sacA' CDS 4249..4911 /label=cat misc_feature 5207..5223 /label=BioBrick prefix ORIGIN 1 actagtatca tctgcagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 61 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 121 ttcgggtggg cctttctgcg tttataggtg ggcctttctg cgtttatatt aagcttctag 181 gatcctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat aataataacc 241 gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact atttcgatca 301 gaccagtttt taatttgtgt gtttccatgt gtccagtttg gcgcgcccca tccgaccatt 361 gactgccact ggattcactg catgacgctg ccgagacagc tgacgctttc aggacagaag 421 ctgattcagc agccgctgcc tgagctaaaa gccatgcgca gaaatgagaa aaaaatacac 481 atcaacatgc atggatcatc tggtgcgctt ccagtggaaa aacctgaaag aactgagatt 541 ctactggaag acattcatac ggagtctggc ttttcaatca gtatccgcgg aacggctacg 601 ttttccttcc ataaagacga ggggattgtt acgctggaac gaaagagctt tgacggaaaa 661 agaacagaag cgagacattg ccgcatcaag gatttgcata ccgtacacat gtttctcgac 721 gcgtcatctg tggaaatctt tatcaataac ggagaagagg tctttagtgc aagatatttt 781 cctttcccgg gaaatcatga agtaacagcc agtgcgaccg ggaaatcctg gcgtaatagc 841 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc 901 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact 961 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc 1021 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc 1081 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga 1141 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 1201 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 1261 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 1321 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 1381 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 1441 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 1501 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 1561 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 1621 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 1681 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 1741 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 1801 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 1861 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 1921 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 1981 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 2041 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 2101 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 2161 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 2221 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 2281 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 2341 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 2401 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 2461 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 2521 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 2581 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 2641 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 2701 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 2761 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 2821 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 2881 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 2941 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 3001 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 3061 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 3121 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 3181 cattaatgca ggacagcaca tgaccaggag cttcgtcgcc gggcttatga agaagtggag 3241 aaaaaagagc ccatcgctaa cagcgatccg caccgccagc attttcatat catgccgccg 3301 gttgggctgc tgaatgaccc gaatggcgtg atttattgga agggcagcta tcatgtattc 3361 tttcagtggc agccgtttca gacggggcac ggcgcaaaat tttgggggca ttatacgaca 3421 caggatgttg tgaattggaa gcgggaagag attgcgctgg ctccgagtga ttggtttgat 3481 aaaaacggct gctactcggg cagcgctgtc acgaaagacg atcggctcta tcttttttac 3541 acaggaaatg tcagggatca ggatggaaat cgggaaacgt atcaatgcct tgctgtttct 3601 gacgacgggc tgtcctttga gaaaaagggt gtcgtcgccc gccttccgga agcgatatta 3661 acggcgcact tttcgcgatc cgaagtatgg gagcatgaag gcacatggta tatggtgatt 3721 ggtgcgcaaa cagagaattt gaaagggcag gctgtgttgt ttgcttctga taacctgaca 3781 gagtggagat ttcttggccc gataaccggc gcgggcttca acgggctgga cgattttggc 3841 gcgcctaaac ggatatcatc atcgctcatc catgtcgacg ctctccctta tgcgactcct 3901 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg 3961 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca 4021 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt 4081 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc 4141 cggcgtagag gatctggagc tgtaatataa aaaccttctt caactaacgg ggcaggttag 4201 tgacattaga aaaccgactg taaaaagtac agtcggcatt atctcatatt ataaaagcca 4261 gtcattaggc ctatctgaca attcctgaat agagttcata aacaatcctg catgataacc 4321 atcacaaaca gaatgatgta cctgtaaaga tagcggtaaa tatattgaat tacctttatt 4381 aatgaatttt cctgctgtaa taatgggtag aaggtaatta ctattattat tgatatttaa 4441 gttaaaccca gtaaatgaag tccatggaat aatagaaaga gaaaaagcat tttcaggtat 4501 aggtgttttg ggaaacaatt tccccgaacc attatatttc tctacatcag aaaggtataa 4561 atcataaaac tctttgaagt cattctttac aggagtccaa ataccagaga atgttttaga 4621 tacaccatca aaaattgtat aaagtggctc taacttatcc caataaccta actctccgtc 4681 gctattgtaa ccagttctaa aagctgtatt tgagtttatc acccttgtca ctaagaaaat 4741 aaatgcaggg taaaatttat atccttcttg ttttatgttt cggtataaaa cactaatatc 4801 aatttctgtg gttatactaa aagtcgtttg ttggttcaaa taatgattaa atatctcttt 4861 tctcttccaa ttgtctaaat caattttatt aaagttcatt tgatatgcct cctaaatttt 4921 tatctaaagt gaatttagga ggcttacttg tctgctttct tcattagaat caatcctttt 4981 ttaaaagtca atattactgt aacataaata tatattttaa aaatatccca ctttatccaa 5041 ttttcgtttg ttgaactaat gggtgcttta gttgaagaat aaagaccaca ttaaaaaatg 5101 tggtcttttg tgttttttta aaggatttga gcgtagcgaa aaatcctttt ctttcttatc 5161 ttgataataa gggtaactat tgccgatgat aagctgtcaa acatgagaat tcacaggtct 5221 aga //