BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K818310 1 BBa_K818310 AmilGFP with alsT regulator 2012-09-23T11:00:00Z 2015-05-08T01:13:29Z BBa_K818300, BBa_K592010, BBa_B0034 This is a coding construct for B. subtilis to produce AmilGFP pigment in response of rotten meat volatiles. AmilCP is a blue pigment designed by iGEM Uppsala 2011 and the regulator, alsT, is designed by iGEM Groningen 2012. false false _1076_ 0 11906 9 It's complicated false A combined part from BBa_K818300, BBa_K592010, and BBa_B0034. false Emeraldo Jo component2220085 1 BBa_K592010 component2220081 1 BBa_K818300 component2220083 1 BBa_B0034 annotation2220083 1 BBa_B0034 range2220083 1 509 520 annotation2220085 1 BBa_K592010 range2220085 1 527 1225 annotation2220081 1 BBa_K818300 range2220081 1 1 500 BBa_K592010 1 amilGFP amilGFP, yellow chromoprotein 2011-09-17T11:00:00Z 2015-05-08T01:12:48Z Acropora millepora This chromoprotein, amilGFP, naturally exhibits very strong yellow color when expressed. The color is strong and readily visible to naked eye both in LB-culture and on agar plates. The DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of two illegal internal restriction sites (EcoRI and PstI). false false _763_ 0 7929 9 It's complicated false Two illegal internal restriction sites (EcoRI and PstI) was removed. false Lei Sun annotation2131633 1 amilGFP range2131633 1 1 696 BBa_K818300 1 P-alsT Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis. 2012-09-18T11:00:00Z 2015-05-08T01:13:29Z This part is from Bacillus subtilis genome. Promoter alsT is a promoter that is repressed by tnrA in the presence of low ammonium by B. subtilis. tnrA is a promoter that responds to ammonium availability in the environment. At low availbility tnrA is activated thus repressing alsT. At high availbility of ammonium (produced by rotten meat), tnrA will be deactivated and alsT will be active. false false _1076_ 0 11906 9 Not in stock false - false Emeraldo Jo annotation2213092 1 alsT promoter range2213092 1 1 500 BBa_K818310_sequence 1 ctccccctcctttaatattactgaatatttttataataataccccaatccatcctaaaagtaaacctgatttattaaatttaaaaataaatttaactttgttaagtatatatctttttgcttatttttgaaagagaaaacttcttttattagcgtatgtatacccggtttatggtgtgaataaaaacatattttaaatgatatgttgtttaggatttattactttatttatgtgtaaatcaaagagaaaggcaagagaatgggaagaaatgattacattgttaatagggtactctttttttaaagccagtttgcaaggaatgtttttattcgtaggaaaataatagagaaggggtcgacagagtaacatctggctatgacaatattcttctcgaaaaaacttcctaattcgtcatattgtgatataataaaactcgttatgttaaaaaatctaacatcaaaatcgaattcgtattgaattgatgaaaacgggaggtaaattactagagaaagaggagaaatactagatgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K818300_sequence 1 ctccccctcctttaatattactgaatatttttataataataccccaatccatcctaaaagtaaacctgatttattaaatttaaaaataaatttaactttgttaagtatatatctttttgcttatttttgaaagagaaaacttcttttattagcgtatgtatacccggtttatggtgtgaataaaaacatattttaaatgatatgttgtttaggatttattactttatttatgtgtaaatcaaagagaaaggcaagagaatgggaagaaatgattacattgttaatagggtactctttttttaaagccagtttgcaaggaatgtttttattcgtaggaaaataatagagaaggggtcgacagagtaacatctggctatgacaatattcttctcgaaaaaacttcctaattcgtcatattgtgatataataaaactcgttatgttaaaaaatctaacatcaaaatcgaattcgtattgaattgatgaaaacgggaggtaaat BBa_K592010_sequence 1 atgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z