LOCUS       BBa_K819004             2783 bp    DNA     linear       25-Nov-2024
DEFINITION  Luminesensor repressible Red luciferase
ACCESSION   BBa_K819004
VERSION     BBa_K819004.1  
FEATURES             Location/Qualifiers
     promoter        1..152
                     /label=ColE promoter
     promoter        1..152
                     /label=BBa_K819003
     RBS             161..177
                     /label=rbs
     CDS             178..1104
                     /label=Luciferin regenerating enzyme (L. cruciata)
     misc_feature    1105..1110
                     /label=double stop codon
     misc_feature    1111..1116
                     /label=BamH1 site
     RBS             1117..1133
                     /label=rbs
     CDS             1134..2777
                     /label=Red luciferase (286) (L. cruciata)
     misc_feature    2778..2783
                     /label=double stop codon
     misc_feature    1990..1990
                     /label=G to A: Ser286Asn
     misc_feature    161..2783
                     /label=BBa_K325210
ORIGIN
        1 tgtttttttg atcgttttca caaaaatgga agtccacagt cttgacaggg aaaatgcagc
       61 ggcgtagctt ttatgctgta tataaaacca gtggttatat gtacagtatt tatttttaac
      121 ttattgtttt aaaagtcaaa gaggatttta tatactagag ttaaggaggt aaaaaaaatg
      181 gccccgaccg tggaacaaat tgtggagctg ggtacgtatc tgctggccga aagcccgcac
      241 tgggacgatg aaacgcaaag cctgtatttc gtcgatatcg ttggtcgcag cgtgaacaaa
      301 tacgttccga cgaccaagac gcatacccaa ctgaaattcg acaagaatcc gagcttcatc
      361 atcccggtga agggttgttc cgaccgtttt atcgtcagcc tggaacgcga gatcaacctg
      421 ctgacctggg acggcgcatc ctctgcacca tccaagattg agaagatcgc ggttttcgat
      481 aatacgccag agaagagcga aaaccgtctg aacgatggta aggcagatcc gctgggcaac
      541 ctgtgggcgg gtaccatgaa tatgggttct gaccatacca ccggcacgcc ggtgcgcggt
      601 accctgagca gcctgagcaa caagcaagtc aaagagcacg tcagcgaggt ctgcatcagc
      661 aatggcctgg catggagcaa ggacctgaag aaattctatt acattgatag cgctgttcgt
      721 caagttgacc agttcgattt cgatgcgaaa aacctgagct tgagcaatcg tcaaccgctg
      781 ttcacctttg acaaacacgg catcatgggt agcccggatg gtcagaccat cgatgcggag
      841 ggtaacctgt gggttgccac ctgtcagggc gataaagtcc tgaagattga tacttctacc
      901 ccggaaaccc tgctgggtat tgtggagatt ccggaacatc aggtgacgag cgtttgcatc
      961 ggcggtgcag aactgaacgt tttgtatgtg acgaccgcgt ccattaaact gcctggtgcg
     1021 gatgaaacca agccgatgaa aggcgccatc tataaagtca ccggtctggg tgtcaaaggt
     1081 ctgccgggtg accgtgttaa gctgtaataa ggatccttaa ggaggtaaaa aaaatggaga
     1141 acatggagaa cgacgagaat attgtggttg gtccgaaacc gttctacccg atcgaagagg
     1201 gcagcgcggg tacccagctg cgtaaataca tggaacgcta cgcgaaactg ggtgcgatcg
     1261 cgttcacgaa tgcggttacc ggcgtggact attcctatgc ggagtatttg gaaaagtcgt
     1321 gttgtctggg caaggcgtta cagaattatg gcctggtggt cgatggccgt atcgcgctgt
     1381 gcagcgagaa ctgtgaagag tttttcattc cggtgatcgc gggtttgttt attggcgtgg
     1441 gcgttgcacc gaccaatgaa atctacacgc tgcgcgaact ggtgcactcc ctgggtatct
     1501 ctaagccgac gatcgttttc agctccaaga aaggtttgga taaagttatt acggtgcaaa
     1561 agacggtcac cacgatcaaa acgattgtga tcctggacag caaagttgat taccgcggtt
     1621 atcaatgcct ggatacgttc atcaagcgta acaccccgcc aggttttcaa gcatctagct
     1681 tcaaaacggt ggaggttgac cgtaaagaac aagtcgcact gattatgaat agctcgggta
     1741 gcacgggcct gccaaagggt gtgcagctga cccatgaaaa caccgttacc cgttttagcc
     1801 acgcgcgtga tccgatttac ggcaatcaag tgtcgccggg tacggctgtt ctgactgttg
     1861 tcccttttca ccatggtttt ggcatgttta ccactctggg ttacctgatt tgcggttttc
     1921 gcgtcgttat gctgaccaag tttgatgaag aaaccttcct gaaaaccctg caagactaca
     1981 agtgcactaa tgtgattctg gtcccgacgc tgttcgcgat cctgaacaaa agcgagctgc
     2041 tgaataagta cgacctgagc aatttggtgg agattgcgag cggcggtgct ccgttgagca
     2101 aagaagtagg cgaggcagtg gcacgtcgct ttaacctgcc tggcgtccgt caaggttacg
     2161 gtttgactga aaccacgagc gcgattatca tcaccccgga aggtgacgat aaacctggcg
     2221 ctagcggtaa ggtcgtgccg ttgttcaagg ctaaagttat tgatctggac accaagaaga
     2281 gcctgggccc gaatcgccgt ggtgaagttt gcgttaaggg cccgatgctg atgaaaggtt
     2341 atgtaaacaa tccggaagca acgaaagagc tgatcgacga agagggctgg ctgcacactg
     2401 gcgatatcgg ctactacgat gaagagaaac actttttcat tgttgatcgc ctgaaaagcc
     2461 tgatcaagta caagggttac caagtcccgc cagcggagct ggaaagcgta ctgttgcagc
     2521 acccgtcgat tttcgacgcc ggtgtggctg gtgtgcctga tccggtggct ggcgaactgc
     2581 cgggtgctgt cgtggtgctg gagagcggta agaatatgac cgaaaaagaa gtcatggact
     2641 atgtggcgag ccaagtgtcc aatgcaaaac gtctgcgtgg cggtgttcgc tttgtggacg
     2701 aagtgccgaa aggtctgacc ggcaaaattg acggtcgtgc aattcgtgag attctgaaga
     2761 aaccggtcgc gaaaatgtaa taa
//