LOCUS BBa_K820008 598 bp DNA linear 31-Oct-2024 DEFINITION LacI-inducible LacZalpha ACCESSION BBa_K820008 VERSION BBa_K820008.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..461 /label=lacZ misc_feature 228..230 /label=START misc_feature 456..461 /label=STOP CDS 228..461 /label=BBa_I732006 stem_loop 481..524 /label=stem_loop misc_feature 470..549 /label=BBa_B0010 terminator 470..549 /label=BBa_B0010 stem_loop 565..584 /label=T7 TE misc_feature 591..591 /label=stop polyA_site 585..598 /label=polya misc_feature 558..598 /label=BBa_B0012 terminator 558..598 /label=BBa_B0012 terminator 470..598 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg accatgatta 241 cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 301 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 361 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc 421 cggcaccaga agcggtgccg gaaagctggc tggagtaata atactagagc caggcatcaa 481 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 541 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //