LOCUS       BBa_K820008              598 bp    DNA     linear       11-Jun-2024
DEFINITION  LacI-inducible LacZalpha
ACCESSION   BBa_K820008
VERSION     BBa_K820008.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..461
                     /label=lacZ
     misc_feature    228..230
                     /label=START
     misc_feature    456..461
                     /label=STOP
     CDS             228..461
                     /label=BBa_I732006
     stem_loop       481..524
                     /label=stem_loop
     misc_feature    470..549
                     /label=BBa_B0010
     terminator      470..549
                     /label=BBa_B0010
     stem_loop       565..584
                     /label=T7 TE
     misc_feature    591..591
                     /label=stop
     polyA_site      585..598
                     /label=polya
     misc_feature    558..598
                     /label=BBa_B0012
     terminator      558..598
                     /label=BBa_B0012
     terminator      470..598
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg accatgatta
      241 cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      301 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      361 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc
      421 cggcaccaga agcggtgccg gaaagctggc tggagtaata atactagagc caggcatcaa
      481 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      541 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//