##gff-version 3 ##sequence-region BBa_K820008 1 598 BBa_K820008 . terminator 470 598 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K820008 . terminator 558 598 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K820008 . polyA_site 585 598 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K820008 . engineered_region 558 598 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K820008 . stem_loop 565 584 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K820008 . stop_codon 591 591 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K820008 . terminator 470 549 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K820008 . engineered_region 470 549 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K820008 . stem_loop 481 524 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K820008 . CDS 228 461 . + 0 ID=BBa_I732006;Name=BBa_I732006 BBa_K820008 . CDS 228 461 . + 0 ID=annotation1936904;Name=lacZ;Parent=BBa_I732006 BBa_K820008 . stop_codon 456 461 . + 0 ID=annotation1936906;Name=STOP;Parent=BBa_I732006 BBa_K820008 . start_codon 228 230 . + 0 ID=annotation1936905;Name=START;Parent=BBa_I732006 BBa_K820008 . ribosome_entry_site 209 221 . + 0 ID=BBa_B0032;Name=BBa_B0032 BBa_K820008 . ribosome_entry_site 209 221 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_K820008 . ribosome_entry_site 215 218 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_K820008 . engineered_region 209 221 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_K820008 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_K820008 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_K820008 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_K820008 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_K820008 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_K820008 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_K820008 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_K820008 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 >BBa_K820008 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgaccatga ttacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagc acatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaat ggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagccagg catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact agagtcacactggctcaccttcgggtgggcctttctgcgtttata