LOCUS       BBa_K820009              670 bp    DNA     linear       29-Jun-2024
DEFINITION  LacI-inducible ccdB
ACCESSION   BBa_K820009
VERSION     BBa_K820009.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     misc_feature    228..230
                     /label=start
     misc_feature    531..533
                     /label=stop
     CDS             228..530
                     /label=cds
     CDS             228..533
                     /label=BBa_K145151
     stem_loop       553..596
                     /label=stem_loop
     misc_feature    542..621
                     /label=BBa_B0010
     terminator      542..621
                     /label=BBa_B0010
     stem_loop       637..656
                     /label=T7 TE
     misc_feature    663..663
                     /label=stop
     polyA_site      657..670
                     /label=polya
     misc_feature    630..670
                     /label=BBa_B0012
     terminator      630..670
                     /label=BBa_B0012
     terminator      542..670
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg cagtttaagg
      241 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
      301 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
      361 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
      421 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
      481 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taatactaga
      541 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      601 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      661 tgcgtttata
//