##gff-version 3
##sequence-region BBa_K820009 1 670
BBa_K820009	.	terminator	542	670	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K820009	.	terminator	630	670	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K820009	.	stem_loop	637	656	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K820009	.	engineered_region	630	670	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K820009	.	stop_codon	663	663	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K820009	.	polyA_site	657	670	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K820009	.	terminator	542	621	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K820009	.	stem_loop	553	596	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K820009	.	engineered_region	542	621	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K820009	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K820009	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K820009	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K820009	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K820009	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K820009	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K820009	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K820009	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K820009	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K820009	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K820009	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K820009	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K820009	.	CDS	228	533	.	+	0	ID=BBa_K145151;Name=BBa_K145151
BBa_K820009	.	start_codon	228	230	.	+	0	ID=annotation1970251;Name=start;Parent=BBa_K145151
BBa_K820009	.	CDS	228	530	.	+	0	ID=annotation1970253;Name=cds;Parent=BBa_K145151
BBa_K820009	.	stop_codon	531	533	.	+	0	ID=annotation1970252;Name=stop;Parent=BBa_K145151
>BBa_K820009
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgcagttta
aggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcg
acggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatc
ggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatc
tcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata