LOCUS       BBa_K821008             1956 bp    DNA     linear       22-Dec-2024
DEFINITION  PLtet+orthogonal 16S RNA
ACCESSION   BBa_K821008
VERSION     BBa_K821008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             63..1775
                     /label=Orthogonal 16S rRNA
     CDS             63..1956
                     /label=BBa_K821002
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agggccgctg agaaaaagcg aagcggcact gctctttaac aatttatcag acaatctgtg
      121 tgggcactcg aagatacgga ttcttaacgt cgcaagacga aaaatgaata ccaagtctca
      181 agagtgaaca cgtaattcat tacgaagttt aattctttga gcgtcaaact tttaaattga
      241 agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgaac
      301 ggtaacagga agaagcttgc ttctttgctg acgagtggcg gacgggtgag taatgtctgg
      361 gaaactgcct gatggagggg gataactact ggaaacggta gctaataccg cataacgtcg
      421 caagaccaaa gagggggacc ttcgggcctc ttgccatcgg atgtgcccag atgggattag
      481 ctagtaggtg gggtaacggc tcacctaggc gacgatccct agctggtctg agaggatgac
      541 cagccacact ggaactgaga cacggtccag actcctacgg gaggcagcag tggggaatat
      601 tgcacaatgg gcgcaagcct gatgcagcca tgccgcgtgt atgaagaagg ccttcgggtt
      661 gtaaagtact ttcagcgggg aggaagggag taaagttaat acctttgctc attgacgtta
      721 cccgcagaag aagcaccggc taactccgtg ccagcagccg cggtaatacg gagggtgcaa
      781 gcgttaatcg gaattactgg gcgtaaagcg cacgcaggcg gtttgttaag tcagatgtga
      841 aatccccggg ctcaacctgg gaactgcatc tgatactggc aagcttgagt ctcgtagagg
      901 ggggtagcat tccaggtgta gcggtgaaat gcgtagagat ctggaggaat accggtggcg
      961 aaggcggccc cctggacgaa gactgacgct caggtgcgaa agcgtgggga gcaaacagga
     1021 ttagataccc tggtagtcca cgccgtaaac gatgtcgact tggaggttgt gcccttgagg
     1081 cgtggcttcc ggagctaacg cgttaagtcg accgcctggg gagtacggcc gcaaggttaa
     1141 aactcaaatg aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgatgc
     1201 aacgcgaaga accttacctg gtcttgacat ccacggaagt tttcagagat gagaatgtgc
     1261 cttcgggaac cgtgagacag gtgctgcatg gctgtcgtca gctcgtgttg tgaaatgttg
     1321 ggttaagtcc cgcaacgagc gcaaccctta tcctttgttg ccagcggtcc ggccgggaac
     1381 tcaaaggaga ctgccagtga taaactggag gaaggtgggg atgacgtcaa gtcatcatgg
     1441 cccttacgac cagggctaca cacgtgctac aatggcgcat acaaagagaa gcgacctcgc
     1501 gagagcaagc ggacctcata aagtgcgtcg tagtccggat tggagtctgc aactcgactc
     1561 catgaagtcg gaatcgctag taatcgtgga tcagaatgcc acggtgaata cgttcccggg
     1621 ccttgtacac accgcccgtc acaccatggg agtgggttgc aaaagaagta ggtagcttaa
     1681 ccttcgggag ggcgcttacc actttgtgat tcatgactgg ggtgaagtcg taacaaggta
     1741 accgtagggg aacctgcggt tggatcacgg aactacctta aagaagcgta ctttgtagtg
     1801 ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt ttacgcgttg ggagtgaggc
     1861 tgaagagaat aaggccgttc gctttctatt aatgaaagct caccctacac gaaaatatca
     1921 cgcaacgcgt gataagcaat tttcgtgtcc ccttcg
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