LOCUS       BBa_K821010             1833 bp    DNA     linear       23-May-2024
DEFINITION  PLacI+Native RBS+RFP+Native RBS+GFP+Terminator
ACCESSION   BBa_K821010
VERSION     BBa_K821010.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..932
                     /label=BBa_E1010
     misc_feature    945..948
                     /label=conserved
     RBS             941..952
                     /label=BBa_B0034
     misc_feature    959..961
                     /label=start
     CDS             959..1672
                     /label=mut3b coding region
     misc_feature    1673..1690
                     /label=6-his tag
     misc_feature    1691..1696
                     /label=stop
     CDS             959..1696
                     /label=BBa_I746918
     stem_loop       1716..1759
                     /label=stem_loop
     misc_feature    1705..1784
                     /label=BBa_B0010
     terminator      1705..1784
                     /label=BBa_B0010
     stem_loop       1800..1819
                     /label=T7 TE
     misc_feature    1826..1826
                     /label=stop
     polyA_site      1820..1833
                     /label=polya
     misc_feature    1793..1833
                     /label=BBa_B0012
     terminator      1793..1833
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag aaagaggaga aatactagat
      961 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1021 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     1081 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     1141 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
     1201 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     1261 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     1321 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     1381 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     1441 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     1501 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     1561 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     1621 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aacatcatca
     1681 ccatcaccac taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1741 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1801 gctcaccttc gggtgggcct ttctgcgttt ata
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