##gff-version 3
##sequence-region BBa_K821010 1 1833
BBa_K821010	.	ribosome_entry_site	941	952	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K821010	.	conserved_region	945	948	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K821010	.	CDS	959	1696	.	+	0	ID=BBa_I746918;Name=BBa_I746918
BBa_K821010	.	stop_codon	1691	1696	.	+	0	ID=annotation1977609;Name=stop;Parent=BBa_I746918
BBa_K821010	.	CDS	959	1672	.	+	0	ID=annotation1977607;Name=mut3b coding region;Parent=BBa_I746918
BBa_K821010	.	tag	1673	1690	.	+	0	ID=annotation1977608;Name=6-his tag;Parent=BBa_I746918
BBa_K821010	.	start_codon	959	961	.	+	0	ID=annotation1977606;Name=start;Parent=BBa_I746918
BBa_K821010	.	CDS	227	932	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K821010	.	engineered_tag	908	932	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K821010	.	CDS	227	901	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K821010	.	terminator	1705	1784	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K821010	.	engineered_region	1705	1784	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K821010	.	stem_loop	1716	1759	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K821010	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K821010	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K821010	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K821010	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K821010	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K821010	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K821010	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K821010	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K821010	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K821010	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K821010	.	terminator	1793	1833	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K821010	.	engineered_region	1793	1833	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K821010	.	stop_codon	1826	1826	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K821010	.	stem_loop	1800	1819	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K821010	.	polyA_site	1820	1833	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K821010
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagagga
gaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaat
gggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcacta
ctggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccaga
tcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagat
gacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattg
attttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcaga
caaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccat
tatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgcccttt
cgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgga
tgaactatacaaacatcatcaccatcaccactaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata