LOCUS       BBa_K821011             1827 bp    DNA     linear       22-May-2024
DEFINITION  PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator
ACCESSION   BBa_K821011
VERSION     BBa_K821011.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..214
                     /label=Orthogonal RBS 1
     RBS             209..214
                     /label=BBa_K821009
     CDS             221..895
                     /label=mrfp1
     misc_feature    902..926
                     /label=Help:Barcodes
     CDS             221..926
                     /label=BBa_E1010
     misc_feature    939..942
                     /label=conserved
     RBS             935..946
                     /label=BBa_B0034
     misc_feature    953..955
                     /label=start
     CDS             953..1666
                     /label=mut3b coding region
     misc_feature    1667..1684
                     /label=6-his tag
     misc_feature    1685..1690
                     /label=stop
     CDS             953..1690
                     /label=BBa_I746918
     stem_loop       1710..1753
                     /label=stem_loop
     misc_feature    1699..1778
                     /label=BBa_B0010
     terminator      1699..1778
                     /label=BBa_B0010
     stem_loop       1794..1813
                     /label=T7 TE
     misc_feature    1820..1820
                     /label=stop
     polyA_site      1814..1827
                     /label=polya
     misc_feature    1787..1827
                     /label=BBa_B0012
     terminator      1787..1827
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggt tccgtactag atggcttcct ccgaagacgt
      241 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
      301 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
      361 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta
      421 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
      481 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
      541 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
      601 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
      661 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
      721 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
      781 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
      841 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata
      901 acgctgatag tgctagtgta gatcgctact agagaaagag gagaaatact agatgcgtaa
      961 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     1021 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     1081 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
     1141 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt
     1201 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
     1261 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     1321 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     1381 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
     1441 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
     1501 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
     1561 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     1621 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaacatc atcaccatca
     1681 ccactaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1741 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1801 cttcgggtgg gcctttctgc gtttata
//