BBa_K821011
type: ComponentDefinition
persistent identity: BBa K821011
display id: BBa_K821011
version: 1
was generated by: iGEM to SBOL conversion
description: PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:29Z
mutable provenance: From BioBrick
owned by: james
owned by: myers
mutable description: PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator
discontinued: false
dominant: false
experience: experience/Works
group u list: _1079_
m user id: 0
owner id: 10617
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: To compare the strength of the orthogonal RBS with the native RBS in expression protein
star: false
creator: Jinlai Zhang, Dongchang Qin
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_I746918
component: BBa_B0034
component: BBa_E1010
component: BBa_K821009
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_K821009
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_I746918
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K821011_sequence/1

BBa_B0012
type: Component
persistent identity: component2269209
display id: component2269209
version: 1
top level: BBa_K821011
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2269207
display id: component2269207
version: 1
top level: BBa_K821011
definition: BBa_B0010
access: public

BBa_I746918
type: Component
persistent identity: component2269206
display id: component2269206
version: 1
top level: BBa_K821011
definition: BBa_I746918
access: public

BBa_B0034
type: Component
persistent identity: component2269201
display id: component2269201
version: 1
top level: BBa_K821011
definition: BBa_B0034
access: public

BBa_E1010
type: Component
persistent identity: component2269199
display id: component2269199
version: 1
top level: BBa_K821011
definition: mRFP1
access: public

BBa_K821009
type: Component
persistent identity: component2269196
display id: component2269196
version: 1
top level: BBa_K821011
definition: BBa_K821009
access: public

BBa_R0010
type: Component
persistent identity: component2269188
display id: component2269188
version: 1
top level: BBa_K821011
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2269188
display id: annotation2269188
version: 1
top level: BBa_K821011
location: https://synbiohub.org/public/igem/BBa_K821011/annotation2269188/range2269188/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K821011/annotation2269188/range2269188/1
type: Range
persistent identity: range2269188
display id: range2269188
version: 1
top level: BBa_K821011
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K821009
type: SequenceAnnotation
persistent identity: annotation2269196
display id: annotation2269196
version: 1
top level: BBa_K821011
location: https://synbiohub.org/public/igem/BBa_K821011/annotation2269196/range2269196/1
component: BBa_K821009

https://synbiohub.org/public/igem/BBa_K821011/annotation2269196/range2269196/1
type: Range
persistent identity: range2269196
display id: range2269196
version: 1
top level: BBa_K821011
direction: direction/reverse
start: 209
end: 214
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2269199
display id: annotation2269199
version: 1
top level: BBa_K821011
location: https://synbiohub.org/public/igem/BBa_K821011/annotation2269199/range2269199/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K821011/annotation2269199/range2269199/1
type: Range
persistent identity: range2269199
display id: range2269199
version: 1
top level: BBa_K821011
direction: direction/reverse
start: 221
end: 926
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2269201
display id: annotation2269201
version: 1
top level: BBa_K821011
location: https://synbiohub.org/public/igem/BBa_K821011/annotation2269201/range2269201/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K821011/annotation2269201/range2269201/1
type: Range
persistent identity: range2269201
display id: range2269201
version: 1
top level: BBa_K821011
direction: direction/reverse
start: 935
end: 946
orientation: inline

BBa_I746918
type: SequenceAnnotation
persistent identity: annotation2269206
display id: annotation2269206
version: 1
top level: BBa_K821011
location: https://synbiohub.org/public/igem/BBa_K821011/annotation2269206/range2269206/1
component: BBa_I746918

https://synbiohub.org/public/igem/BBa_K821011/annotation2269206/range2269206/1
type: Range
persistent identity: range2269206
display id: range2269206
version: 1
top level: BBa_K821011
direction: direction/reverse
start: 953
end: 1690
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2269207
display id: annotation2269207
version: 1
top level: BBa_K821011
location: https://synbiohub.org/public/igem/BBa_K821011/annotation2269207/range2269207/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K821011/annotation2269207/range2269207/1
type: Range
persistent identity: range2269207
display id: range2269207
version: 1
top level: BBa_K821011
direction: direction/forward
start: 1699
end: 1778
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2269209
display id: annotation2269209
version: 1
top level: BBa_K821011
location: https://synbiohub.org/public/igem/BBa_K821011/annotation2269209/range2269209/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K821011/annotation2269209/range2269209/1
type: Range
persistent identity: range2269209
display id: range2269209
version: 1
top level: BBa_K821011
direction: direction/forward
start: 1787
end: 1827
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_I746918
type: ComponentDefinition
persistent identity: BBa I746918
display id: BBa_I746918
version: 1
was generated by: iGEM to SBOL conversion
description: mut3b GFP with 6-his tag
created: 2008-09-29T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: The part was obtained by PCR from mut3b GFP from the 2007 parts distribution.
owned by: james
owned by: myers
mutable description: Coding region of mut3b with C-terminal 6-his tag for assembly into constructs that allow 6-his purification of mut3b GFP.
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: 2 stop codons were removed from the mut3b coding sequence and reinserted after the 6-his tag.
star: false
creator: Stefan Milde
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mut3b coding region
sequence annotation: start
sequence annotation: 6-his tag
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I746918_sequence/1

mut3b coding region
type: SequenceAnnotation
persistent identity: annotation1977607
display id: annotation1977607
version: 1
top level: BBa_I746918
location: https://synbiohub.org/public/igem/BBa_I746918/annotation1977607/range1977607/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_I746918/annotation1977607/range1977607/1
type: Range
persistent identity: range1977607
display id: range1977607
version: 1
top level: BBa_I746918
direction: direction/forward
start: 1
end: 714
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1977606
display id: annotation1977606
version: 1
top level: BBa_I746918
location: https://synbiohub.org/public/igem/BBa_I746918/annotation1977606/range1977606/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I746918/annotation1977606/range1977606/1
type: Range
persistent identity: range1977606
display id: range1977606
version: 1
top level: BBa_I746918
direction: direction/forward
start: 1
end: 3
orientation: inline

6-his tag
type: SequenceAnnotation
persistent identity: annotation1977608
display id: annotation1977608
version: 1
top level: BBa_I746918
location: https://synbiohub.org/public/igem/BBa_I746918/annotation1977608/range1977608/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_I746918/annotation1977608/range1977608/1
type: Range
persistent identity: range1977608
display id: range1977608
version: 1
top level: BBa_I746918
direction: direction/forward
start: 715
end: 732
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1977609
display id: annotation1977609
version: 1
top level: BBa_I746918
location: https://synbiohub.org/public/igem/BBa_I746918/annotation1977609/range1977609/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I746918/annotation1977609/range1977609/1
type: Range
persistent identity: range1977609
display id: range1977609
version: 1
top level: BBa_I746918
direction: direction/forward
start: 733
end: 738
orientation: inline

BBa_K821009
type: ComponentDefinition
persistent identity: BBa K821009
display id: BBa_K821009
version: 1
was generated by: iGEM to SBOL conversion
description: Orthogonal RBS 1
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:29Z
mutable provenance: PCR mutation
owned by: james
owned by: myers
mutable description: Orthogonal Shine-Dalgarno sequence 1
discontinued: false
dominant: false
experience: experience/Works
group u list: _1079_
m user id: 0
owner id: 10617
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: From our own design, based on ribosome-RBS interaction model
star: false
creator: Qinfeng Wu, Bin Jia
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: Orthogonal RBS 1
sequence: https://synbiohub.org/public/igem/BBa_K821009_sequence/1

Orthogonal RBS 1
type: SequenceAnnotation
persistent identity: annotation2184200
display id: annotation2184200
version: 1
top level: BBa_K821009
location: https://synbiohub.org/public/igem/BBa_K821009/annotation2184200/range2184200/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K821009/annotation2184200/range2184200/1
type: Range
persistent identity: range2184200
display id: range2184200
version: 1
top level: BBa_K821009
direction: direction/forward
start: 1
end: 6
orientation: inline

https://synbiohub.org/public/igem/BBa_K821011_sequence/1
type: Sequence
persistent identity: BBa K821011 sequence
display id: BBa_K821011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagaggttccgtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacatcatcaccatcaccactaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746918_sequence/1
type: Sequence
persistent identity: BBa I746918 sequence
display id: BBa_I746918_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacatcatcaccatcaccactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K821009_sequence/1
type: Sequence
persistent identity: BBa K821009 sequence
display id: BBa_K821009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttccg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z