BBa_K823020 1 BBa_K823020 chloramphenicol acetyltransferase (cat) 2012-08-14T11:00:00Z 2015-05-08T01:13:30Z Plasmid: Bacillus subtilis reporter vector pAC6 This part codes for the chloramphenicol acetyltransferase (cat) gene used in Bacillus subtilis for resistance against the antibiotic chloramphenicol. We constructed it in the freiburg standard (Assembly 25) for in-frame fusion-proteins. There is a Shine-Dalgarno Sequence (RBS) optimized for Bacillus subtilis included between the XbaI and NgoIV restriction site. You can use it both N-and C terminal fusions. false false _1081_ 0 11555 9 It's complicated false We constructed the part in the freiburg standard (Assembly 25). We included a Bacillus optimized RBS between the XbaI and NgoIV restriction site. false Simon Kelterborn annotation2179570 1 cat range2179570 1 1 645 BBa_K823020_sequence 1 aactttaataaaattgatttagacaattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaactggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcatttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaataaaggtaattcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgcaggattgtttatgaactctattcaggaattgtcagataggcctaatgactggctttta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z