LOCUS BBa_K823021 9792 bp DNA linear 22-Nov-2024 DEFINITION pSBBs1C-lacZ (lacZ reporter vector for B. subtilis) ACCESSION BBa_K823021 VERSION BBa_K823021.1 FEATURES Location/Qualifiers CDS 49..3112 /label=lacZ misc_feature 3165..3698 /label=amyE' CDS 4023..4883 /label=bla misc_feature 7402..8229 /label='amyE CDS 8813..9463 /label=cat ORIGIN 1 tactagtagc ggccgctgca gggatccaca gtacataaaa aaggagacat gacgatggtc 61 gttttacaac gtgactgggt cgaccgggaa aaccctggcg ttacccaact taatcgcctt 121 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 181 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa 241 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc 301 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt 361 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt 421 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac 481 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg 541 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg 601 gtgctgcgtt ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc 661 attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt 721 gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc 781 ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc 841 gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat 901 cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat 961 ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc 1021 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag 1081 ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg 1141 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg 1201 cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg 1261 tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc 1321 gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat 1381 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat 1441 cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa 1501 ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat 1561 gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct 1621 ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc 1681 ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc 1741 tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct 1801 tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc 1861 tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc 1921 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc 1981 gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa 2041 gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag 2101 ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca 2161 tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt 2221 gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt 2281 tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag 2341 atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca 2401 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc 2461 gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca 2521 gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa 2581 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc 2641 gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag 2701 ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc 2761 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc 2821 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc 2881 ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg 2941 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac 3001 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaatta 3061 attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata 3121 accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttcga 3181 tcagaccagt ttttaatttg tgtgtttcca tgtgtccagt ttggaatact cttaacctca 3241 ttggaaatcg cggcataatc actggtggta tgattgatga ccgcgtcaac aatgaccttt 3301 atgccatatt cttcagcggc tgcacacatt tctttaaatt cttgttcagt acctaagtaa 3361 cggttgccaa tttgatacga tgtcggctga tacagccagt accagttcga catgctttta 3421 tctccttgat tcccttcctt tacttggtta atcggagatg tctgaatggc tgtatatcct 3481 gcatcatgaa tatccttcat attgtgtttt aacgtattga acgaccaatt ccatgcatga 3541 agaatggttc cgcttttgat cgacggtgct gtaagctcat tcgatttgtt cgccgtttca 3601 gcactcgcag ccgccggtcc tgccagaacc aaatgaaaca gcaataaaaa tccagcgaat 3661 aacggcagta aagaggtttt gaatcgtttt gcaaacattc ttgacactcc ttatttgatt 3721 ttttgaagac ttacttcgga gtcaaaaatc cctcttactt cattcttccg cttcctcctt 3781 tcaaaccgat gtgaagactg gagaattttg ttaattcttg aagacgaaag ggcctcgtga 3841 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 3901 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 3961 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 4021 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 4081 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 4141 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 4201 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 4261 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 4321 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 4381 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 4441 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 4501 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 4561 tgccggcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 4621 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 4681 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 4741 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 4801 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 4861 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 4921 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 4981 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 5041 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 5101 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 5161 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 5221 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 5281 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 5341 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 5401 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 5461 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 5521 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 5581 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 5641 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 5701 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 5761 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 5821 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat 5881 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct 5941 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc 6001 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 6061 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag 6121 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc 6181 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc 6241 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt 6301 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc 6361 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag 6421 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg 6481 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg 6541 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc 6601 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta 6661 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg 6721 cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc 6781 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa 6841 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt 6901 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat 6961 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc 7021 gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct 7081 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc 7141 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc 7201 ggctccggcg caaatgcaga caatatcagc atccttgcag ggtatgtttc tctttgatgt 7261 ctttttgttt gtgaagtatt tcacatttat attgtgcaac acttcacaaa cttttgcaag 7321 agaaaagttt tgtctgattt atgaacaaaa aagaaaccat cattgatggt ttctttcggt 7381 aagtcccgtc tagccttgcc ctcaatgggg aagagaaccg cttaagcccg agtcattata 7441 taaaccattt agcacgtaat caaagccagg ctgattctga ccgggcactt gggcgctgcc 7501 attattaaaa atcacttttg cgttggttgt atccgtgtcc gcaggcagcg tcagcgtgta 7561 aattccgtct gcatttttag tcattggttt tccaggccaa gatccggtca attcaattac 7621 tcggctccca tcatgtttat agatataagc atttacctgg ctccaatgat tcggattttg 7681 atagccgatg gttttggccg acgctggatc tcttttaaca aaactgtatt tctcggtcct 7741 cgttacacca tcactgttcg ttccttttaa catgatggtg tatgttttgc caaattggat 7801 ctccttttcc gattgtgaat tgatctccat ccttaaacgc ctgtcgtctg gtccattatt 7861 gatttgataa acggcttttg ttgtattcgc atctgcacgc aaggtaatcg tcagttgatc 7921 attgaaagaa tgtgttacac ctgttttgta attctcaagg aaaacatgag gcgcttttgc 7981 aatatcatca ggataaagca cagctacaga cctggcattg atcgtgcctg tcagtttacc 8041 atcgttcact tgaaatgaac ccgctccagc tttattgtca tacctgccat caggcaattt 8101 tgttgccgta ttgatagaga cagaggatga acctgcattt gccagcacaa cgccatgtga 8161 gccgcgctga ttcataaata tctggttgtt tccattcggg ttcgagagtt cctcaggctg 8221 tccagccatc acattgtgaa atctattgac cgcagtgata gcctgatctt caaataaagc 8281 actcccgcga tcgcctattt ggcttttccc ccagatctgg ggaacctcac accatttccg 8341 cctccctcag gtctggaaaa gaaaagaggc gtactgcctg aacgagaagc tatcaccgcc 8401 cagcctaaac ggatatcatc atcgctcatc catgtcgatc gacgctctcc cttatgcgac 8461 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg 8521 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata 8581 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg 8641 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg 8701 cgtccggcgt agaggatctg gagctgtaat ataaaaacct tcttcaacta acggggcagg 8761 ttagtgacat tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa 8821 gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat 8881 aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt 8941 tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat 9001 ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag 9061 gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt 9121 ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt 9181 tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc 9241 cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga 9301 aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa 9361 tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct 9421 cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa 9481 tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc 9541 ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat 9601 ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac cacattaaaa 9661 aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc ttttctttct 9721 tatcttgata ataagggtaa ctattgccga tgataagctg tcaaacatga gaattcgcgg 9781 ccgcttctag ag //