LOCUS       BBa_K823024             4794 bp    DNA     linear       06-Dec-2025
DEFINITION  pSB<sub>Bs</sub>4S-P<i><sub>xyl</i></sub>: Integrative expression 
            vector for <i>Bacillus subtilis</i
ACCESSION   BBa_K823024
VERSION     BBa_K823024.1  
FEATURES             Location/Qualifiers
     CDS             211..885
                     /label=spc
     misc_feature    1028..1456
                     /label='thrC
     misc_feature    1453..1863
                     /label=thrB
     CDS             2716..3576
                     /label=bla
     misc_feature    3731..4033
                     /label=hom
     misc_feature    4033..4572
                     /label=thrC'
     promoter        4539..4763
                     /label=Pxly region
ORIGIN
        1 tactagtagc ggccgctgca ggataaaaaa tttagaagcc aatgaaatct ataaataaac
       61 taaattaagt ttatttaatt aacaactatg gatataaaat aggtactaat caaaatagtg
      121 aggaggatat atttgaatac atacgaacaa attaataaag tgaaaaaaat acttcggaaa
      181 catttaaaaa ataaccttat tggtacttac atgtttggat caggagttga gagtggacta
      241 aaaccaaata gtgatcttga ctttttagtc gtcgtatctg aaccattgac agatcaaagt
      301 aaagaaatac ttatacaaaa aattagacct atttcaaaaa aaataggaga taaaagcaac
      361 ttacgatata ttgaattaac aattattatt cagcaagaaa tggtaccgtg gaatcatcct
      421 cccaaacaag aatttattta tggagaatgg ttacaagagc tttatgaaca aggatacatt
      481 cctcagaagg aattaaattc agatttaacc ataatgcttt accaagcaaa acgaaaaaat
      541 aaaagaatat acggaaatta tgacttagag gaattactac ctgatattcc attttctgat
      601 gtgagaagag ccattatgga ttcgtcagag gaattaatag ataattatca ggatgatgaa
      661 accaactcta tattaacttt atgccgtatg attttaacta tggacacggg taaaatcata
      721 ccaaaagata ttgcgggaaa tgcagtggct gaatcttctc cattagaaca tagggagaga
      781 attttgttag cagttcgtag ttatcttgga gagaatattg aatggactaa tgaaaatgta
      841 aatttaacta taaactattt aaataacaga ttaaaaaaat tataaaaaaa ttgaaaaaat
      901 ggtggaaaca cttttttcaa tttttttgtt ttattattta atatttggga aatattcatt
      961 ctaattggta atcagatttt agaaaacaat aaacccttgc atagggggat ctcgaggcct
     1021 tccgaaaatg cgcggctttg aagctgaggg tgcggcggca atcgtgcgca atgaagtgat
     1081 tgaaaatccg gaaacaatag cgacagccat tcgtatcgga aacccggcaa gctgggacaa
     1141 agctgtaaag gcagccgagg aatccaatgg gaaaattgac gaagtcactg atgatgaaat
     1201 ccttcacgca tatcagctga tcgcccgtgt agaaggcgtg tttgcagaac caggttcttg
     1261 cgcgtctatc gcaggagtgc tgaaacaggt gaaatccgga gaaattccga aaggcagcaa
     1321 ggtcgtagct gtgttaacag gaaacggact gaaagatccg aacacagcgg tcgacatttc
     1381 agaaatcaag cctgtcacat tgccgactga tgaagacagc atccttgaat atgtaaaagg
     1441 agcggcccgt gtatgaacga agccgacatg ctgttctctg tcactgttcc cggaagcaca
     1501 gctaacctag gccccggctt tgattcagtc ggaatggcgc tcagcagata tttgaagctg
     1561 accgtctttg aaagcgacaa atggtctttt gaggctgaaa cagaaacagt cgccggaatt
     1621 aattcgagct cctttaactt cctcaccagt agttgtatcg gtaccataag tagaagcagc
     1681 aacccaagta gctttaccag catccggttc aaccagcata gtaagaatct tactggacat
     1741 cggcagttct tcgaacagtg cgccaactac cagctctttc tacagttcat tcagggcacc
     1801 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     1861 tgatccatgg attacgcgcg accttcagca tcgccggcat gtccccctgg cggacgggaa
     1921 gtatccagct cgaggtcggg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1981 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2041 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2101 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2161 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2221 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2281 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2341 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     2401 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttgatagctc
     2461 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2521 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2581 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2641 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2701 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2761 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2821 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2881 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2941 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3001 taatagtttg cgcaacgttg ttgccattgc tgccggcatc gtggtgtcac gctcgtcgtt
     3061 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3121 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3181 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3241 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3301 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     3361 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3421 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3481 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3541 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3601 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3661 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     3721 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     3781 agaattaatt catgtaaaag atgaggttgg ttcattctcg aaaattacat ctgtgttctc
     3841 agagcggggc gtgacgtttg aaaaaatcct tcagctgcca attaaaggcc atgatgagtt
     3901 agctgaaatc gtaattgtca cacatcatac atcagaagct gatttcagtg atatcctgca
     3961 aaacctaaat gatttggaag tcgttcaaga agtcaaaagc acatatcgtg tagaagggaa
     4021 cggttggagc taatgtggaa aggacttatc catcaatata aagaattttt acctgtaaca
     4081 gatcaaacac cggcgctaac tttacatgaa ggaaacacac ctcttattca cctgccgaag
     4141 ctgtctgagc agctcggaat tgagcttcat gtcaaaacgg aaggcgtcaa tcctacggga
     4201 tcatttaaag atcgcggaat ggttatggct gtggcaaagg caaaagaaga aggcaatgac
     4261 acgattatgt gcgcgtcaac aggtaacact tccgctgctg cggcagcata tgcagcccgt
     4321 gctaacatga aatgcattgt catcatcccg aacggaaaaa ttgcatttgg aaaactcgct
     4381 caagctgtca tgtacggagc cgagattatc gcaattgacg gaaactttga cgatgcgctt
     4441 aaaattgtcc gttccatctg tgagaaatca ccgattgccc ttgtcaactc agtcaaccct
     4501 taccgcattg aaggccaaaa aactgctgcc ttcggatcag cgatatccac ttcatccact
     4561 ccatttgttt aatctttaaa ttaagtatca acatagtaca tagcgaatct tccctttatt
     4621 atatctaatg tgttcataaa aaactaaaaa aaatattgaa aatactgacg aggttatata
     4681 agatgaaaat aagttagttt gtttaaacaa caaactaata ggtgatgtac ttactatatg
     4741 aaataaaatg catctgggat cccaagctta tcgaattcgc ggccgcttct agag
//