LOCUS BBa_K823025 10640 bp DNA linear 22-Nov-2024 DEFINITION pSBBs3C-luxABCDE (lux reporter vector for B.subtilis) ACCESSION BBa_K823025 VERSION BBa_K823025.1 FEATURES Location/Qualifiers CDS 73..1155 /label=luxA CDS 1172..2155 /label=luxB CDS 2179..3621 /label=luxC CDS 3635..4558 /label=luxD CDS 4583..5695 /label=luxE misc_feature 5676..6515 /label=sacA CDS 6849..7721 /label=bla misc_feature 8712..9503 /label=sacA CDS 9661..10323 /label=cat ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcctagt aaggtcgaca 61 ggaggactct ctatgaaatt tggaaacttt ttgcttacat accaacctcc ccaattttct 121 caaacagagg taatgaaacg tttggttaaa ttaggtcgca tctctgagga gtgtggtttt 181 gataccgtat ggttactgga gcatcatttc acggagtttg gtttgcttgg taacccttat 241 gtcgctgctg catatttact tggcgcgact aaaaaattga atgtaggaac tgccgctatt 301 gttcttccca cagcccatcc agtacgccaa cttgaagatg tgaatttatt ggatcaaatg 361 tcaaaaggac gatttcggtt tggtatttgc cgagggcttt acaacaagga ctttcgcgta 421 ttcggcacag atatgaataa cagtcgcgcc ttagcggaat gctggtacgg gctgataaag 481 aatggcatga cagagggata tatggaagct gataatgaac atatcaagtt ccataaggta 541 aaagtaaacc ccgcggcgta tagcagaggt ggcgcaccgg tttatgtggt ggctgaatca 601 gcttcgacga ctgagtgggc tgctcaattt ggcctaccga tgatattaag ttggattata 661 aatactaacg aaaagaaagc acaacttgag ctttataatg aagtggctca agaatatggg 721 cacgatattc ataatatcga ccattgctta tcatatataa catctgtaga tcatgactca 781 attaaagcga aagagatttg ccggaaattt ctggggcatt ggtatgattc ttatgtgaat 841 gctacgacta tttttgatga ttcagaccaa acaagaggtt atgatttcaa taaagggcag 901 tggcgtgact ttgtattaaa aggacataaa gatactaatc gccgtattga ttacagttac 961 gaaatcaatc ccgtgggaac gccgcaggaa tgtattgaca taattcaaaa agacattgat 1021 gctacaggaa tatcaaatat ttgttgtgga tttgaagcta atggaacagt agacgaaatt 1081 attgcttcca tgaagctctt ccagtctgat gtcatgccat ttcttaaaga aaaacaacgt 1141 tcgctattat attagctaag gaggtaaaga aatgaaattt ggattgttct tccttaactt 1201 catcaattca acaactgttc aagaacaaag tatagttcgc atgcaggaaa taacggagta 1261 tgttgataag ttgaattttg aacagatttt agtgtatgaa aatcattttt cagataatgg 1321 tgttgtcggc gctcctctga ctgtttctgg ttttctgctc ggtttaacag agaaaattaa 1381 aattggttca ttaaatcaca tcattacaac tcatcatcct gtcgccatag cggaggaagc 1441 ttgcttattg gatcagttaa gtgaagggag atttatttta gggtttagtg attgcgaaaa 1501 aaaagatgaa atgcattttt ttaatcgccc ggttgaatat caacagcaac tatttgaaga 1561 gtgttatgaa atcattaacg atgctttaac aacaggctat tgtaatccag ataacgattt 1621 ttatagcttc cctaaaatat ctgtaaatcc ccatgcttat acgccaggcg gacctcggaa 1681 atatgtaaca gcaaccagtc atcatattgt tgagtgggcg gccaaaaaag gtattcctct 1741 catctttaag tgggatgatt ctaatgatgt tagatatgaa tatgctgaaa gatataaagc 1801 cgttgcggat aaatatgacg ttgacctatc agagatagac catcagttaa tgatattagt 1861 taactataac gaagatagta ataaagctaa acaagagacg cgtgcattta ttagtgatta 1921 tgttcttgaa atgcacccta atgaaaattt cgaaaataaa cttgaagaaa taattgcaga 1981 aaacgctgtc ggaaattata cggagtgtat aactgcggct aagttggcaa ttgaaaagtg 2041 tggtgcgaaa agtgtattgc tgtcctttga accaatgaat gatttgatga gccaaaaaaa 2101 tgtaatcaat attgttgatg ataatattaa gaagtaccac atggaatata cctaataggt 2161 accaggagga aggcaaatat gactaaaaaa atttcattca ttattaacgg ccaggttgaa 2221 atctttcccg aaagtgatga tttagtgcaa tccattaatt ttggtgataa tagtgtttac 2281 ctgccaatat tgaatgactc tcatgtaaaa aacattattg attgtaatgg aaataacgaa 2341 ttacggttgc ataacattgt caattttctc tatacggtag ggcaaagatg gaaaaatgaa 2401 gaatactcaa gacgcaggac atacattcgt gacttaaaaa aatatatggg atattcagaa 2461 gaaatggcta agctagaggc caattggata tctatgattt tatgttctaa aggcggcctt 2521 tatgatgttg tagaaaatga acttggttct cgccatatca tggatgaatg gctacctcag 2581 gatgaaagtt atgttcgggc ttttccgaaa ggtaaatctg tacatctgtt ggcaggtaat 2641 gttccattat ctgggatcat gtctatatta cgcgcaattt taactaagaa tcagtgtatt 2701 ataaaaacat cgtcaaccga tccttttacc gctaatgcat tagcgttaag ttttattgat 2761 gtagacccta atcatccgat aacgcgctct ttatctgtta tatattggcc ccaccaaggt 2821 gatacatcac tcgcaaaaga aattatgcga catgcggatg ttattgtcgc ttggggaggg 2881 ccagatgcga ttaattgggc ggtagagcat gcgccatctt atgctgatgt gattaaattt 2941 ggttctaaaa agagtctttg cattatcgat aatcctgttg atttgacgtc cgcagcgaca 3001 ggtgcggctc atgatgtttg tttttacgat cagcgagctt gtttttctgc ccaaaacata 3061 tattacatgg gaaatcatta tgaggaattt aagttagcgt tgatagaaaa acttaatcta 3121 tatgcgcata tattaccgaa tgccaaaaaa gattttgatg aaaaggcggc ctattcttta 3181 gttcaaaaag aaagcttgtt tgctggatta aaagtagagg tggatattca tcaacgttgg 3241 atgattattg agtcaaatgc aggtgtggaa tttaatcaac cacttggcag atgtgtgtac 3301 cttcatcacg tcgataatat tgagcaaata ttgccttatg ttcaaaaaaa taagacgcaa 3361 accatatcta tttttccttg ggagtcatca tttaaatatc gagatgcgtt agcattaaaa 3421 ggtgcggaaa ggattgtaga agcaggaatg aataacatat ttcgagttgg tggatctcat 3481 gacggaatga gaccgttgca acgattagtg acatatattt ctcatgaaag gccatctaac 3541 tatacggcta aggatgttgc ggttgaaata gaacagactc gattcctgga agaagataag 3601 ttccttgtat ttgtcccata atggaggtaa aagtatggaa aatgaatcaa aatataaaac 3661 catcgaccac gttatttgtg ttgaaggaaa taaaaaaatt catgtttggg aaacgctgcc 3721 agaagaaaac agcccaaaga gaaagaatgc cattattatt gcgtctggtt ttgcccgcag 3781 gatggatcat tttgctggtc tggcggaata tttatcgcgg aatggatttc atgtgatccg 3841 ctatgattcg cttcaccacg ttggattgag ttcagggaca attgatgaat ttacaatgtc 3901 tataggaaag cagagcttgt tagcagtggt tgattggtta actacacgaa aaataaataa 3961 cttcggtatg ttggcttcaa gcttatctgc gcggatagct tatgcaagcc tatctgaaat 4021 caatgcttcg tttttaatca ccgcagtcgg tgttgttaac ttaagatatt ctcttgaaag 4081 agctttaggg tttgattatc tcagtctacc cattaatgaa ttgccggata atcttgattt 4141 tgaaggccat aaattgggtg ctgaagtctt tgcgagagat tgtcttgatt ttggttggga 4201 agatttagct tctacaatta ataacatgat gtatcttgat ataccgttta ttgcttttac 4261 tgcaaataac gataattggg tcaagcaaga tgaagttatc acattgttat caaatattcg 4321 tagtaatcga tgcaagatat attctttgtt aggaagttcg catgacttga gtgaaaattt 4381 agtggtcctg cgcaattttt atcaatcggt tacgaaagcc gctatcgcga tggataatga 4441 tcatctggat attgatgttg atattactga accgtcattt gaacatttaa ctattgcgac 4501 agtcaatgaa cgccgaatga gaattgagat tgaaaatcaa gcaatttctc tgtcttaaag 4561 aatcctgagg aggaaaacag gtatgacttc atatgttgat aaacaagaaa ttacagcaag 4621 ctcagaaatt gatgatttga ttttttcgag cgatccatta gtgtggtctt acgacgagca 4681 ggaaaaaatc agaaagaaac ttgtgcttga tgcatttcgt aatcattata aacattgtcg 4741 agaatatcgt cactactgtc aggcacacaa agtagatgac aatattacgg aaattgatga 4801 catacctgta ttcccaacat cggtttttaa gtttactcgc ttattaactt ctcaggaaaa 4861 cgagattgaa agttggttta ccagtagcgg cacgaatggt ttaaaaagtc aggtggcgcg 4921 tgacagatta agtattgaga gactcttagg ctctgtgagt tatggcatga aatatgttgg 4981 tagttggttt gatcatcaaa tagaattagt caatttggga ccagatagat ttaatgctca 5041 taatatttgg tttaaatatg ttatgagttt ggtggaattg ttatatccta cgacatttac 5101 cgtaacagaa gaacgaatag attttgttaa aacattgaat agtcttgaac gaataaaaaa 5161 tcaagggaaa gatctttgtc ttattggttc gccatacttt atttatttac tctgccatta 5221 tatgaaagat aaaaaaatct cattttctgg agataaaagc ctttatatca taaccggagg 5281 cggctggaaa agttacgaaa aagaatctct gaaacgtgat gatttcaatc atcttttatt 5341 tgatactttc aatctcagtg atattagtca gatccgagat atatttaatc aagttgaact 5401 caacacttgt ttctttgagg atgaaatgca gcgtaaacat gttccgccgt gggtatatgc 5461 gcgagcgctt gatcctgaaa cgttgaaacc tgtacctgat ggaacgccgg ggttgatgag 5521 ttatatggat gcgtcagcaa ccagttatcc agcatttatt gttaccgatg atgtcgggat 5581 aattagcaga gaatatggta agtatcccgg cgtgctcgtt gaaattttac gtcgcgtcaa 5641 tacgaggacg cagaaagggt gtgctttaag cttaaccgaa gcgtttgata gttgatatcc 5701 agcaggatcc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa 5761 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactatttc 5821 gatcagacca gtttttaatt tgtgtgtttc catgtgtcca gtttggcgcg ccccatccga 5881 ccattgactg ccactggatt cactgcatga cgctgccgag acagctgacg ctttcaggac 5941 agaagctgat tcagcagccg ctgcctgagc taaaagccat gcgcagaaat gagaaaaaaa 6001 tacacatcaa catgcatgga tcatctggtg cgcttccagt ggaaaaacct gaaagaactg 6061 agattctact ggaagacatt catacggagt ctggcttttc aatcagtatc cgcggaacgg 6121 ctacgttttc cttccataaa gacgagggga ttgttacgct ggaacgaaag agctttgacg 6181 gaaaaagaac agaagcgaga cattgccgca tcaaggattt gcataccgta cacatgtttc 6241 tcgacgcgtc atctgtggaa atctttatca ataacggaga agaggtcttt agtgcaagat 6301 attttccttt cccgggaaat catgaagtaa cagccagtgc gaccgggaaa tcctggcgta 6361 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 6421 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt 6481 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 6541 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 6601 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 6661 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 6721 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 6781 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 6841 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 6901 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 6961 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 7021 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 7081 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 7141 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 7201 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 7261 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 7321 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 7381 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 7441 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 7501 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 7561 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 7621 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 7681 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 7741 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 7801 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 7861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 7921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 7981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 8041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 8101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 8161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 8221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 8281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 8341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 8401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 8461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 8521 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 8581 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 8641 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 8701 cgattcatta atgcaggaca gcacatgacc aggagcttcg tcgccgggct tatgaagaag 8761 tggagaaaaa agagcccatc gctaacagcg atccgcaccg ccagcatttt catatcatgc 8821 cgccggttgg gctgctgaat gacccgaatg gcgtgattta ttggaagggc agctatcatg 8881 tattctttca gtggcagccg tttcagacgg ggcacggcgc aaaattttgg gggcattata 8941 cgacacagga tgttgtgaat tggaagcggg aagagattgc gctggctccg agtgattggt 9001 ttgataaaaa cggctgctac tcgggcagcg ctgtcacgaa agacgatcgg ctctatcttt 9061 tttacacagg aaatgtcagg gatcaggatg gaaatcggga aacgtatcaa tgccttgctg 9121 tttctgacga cgggctgtcc tttgagaaaa agggtgtcgt cgcccgcctt ccggaagcga 9181 tattaacggc gcacttttcg cgatccgaag tatgggagca tgaaggcaca tggtatatgg 9241 tgattggtgc gcaaacagag aatttgaaag ggcaggctgt gttgtttgct tctgataacc 9301 tgacagagtg gagatttctt ggcccgataa ccggcgcggg cttcaacggg ctggacgatt 9361 ttggcgcgcc taaacggatc aaggagatgg cgcccaacag tcccccggcc acggggcctg 9421 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc 9481 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg 9541 ccacgatgcg tccggcgtag aggatctgga gctgtaatat aaaaaccttc ttcaactaac 9601 ggggcaggtt agtgacatta gaaaaccgac tgtaaaaagt acagtcggca ttatctcata 9661 ttataaaagc cagtcattag gcctatctga caattcctga atagagttca taaacaatcc 9721 tgcatgataa ccatcacaaa cagaatgatg tacctgtaaa gatagcggta aatatattga 9781 attaccttta ttaatgaatt ttcctgctgt aataatgggt agaaggtaat tactattatt 9841 attgatattt aagttaaacc cagtaaatga agtccatgga ataatagaaa gagaaaaagc 9901 attttcaggt ataggtgttt tgggaaacaa tttccccgaa ccattatatt tctctacatc 9961 agaaaggtat aaatcataaa actctttgaa gtcattcttt acaggagtcc aaataccaga 10021 gaatgtttta gatacaccat caaaaattgt ataaagtggc tctaacttat cccaataacc 10081 taactctccg tcgctattgt aaccagttct aaaagctgta tttgagttta tcacccttgt 10141 cactaagaaa ataaatgcag ggtaaaattt atatccttct tgttttatgt ttcggtataa 10201 aacactaata tcaatttctg tggttatact aaaagtcgtt tgttggttca aataatgatt 10261 aaatatctct tttctcttcc aattgtctaa atcaatttta ttaaagttca tttgatatgc 10321 ctcctaaatt tttatctaaa gtgaatttag gaggcttact tgtctgcttt cttcattaga 10381 atcaatcctt ttttaaaagt caatattact gtaacataaa tatatatttt aaaaatatcc 10441 cactttatcc aattttcgtt tgttgaacta atgggtgctt tagttgaaga ataaagacca 10501 cattaaaaaa tgtggtcttt tgtgtttttt taaaggattt gagcgtagcg aaaaatcctt 10561 ttctttctta tcttgataat aagggtaact attgccgatg ataagctgtc aaacatgaga 10621 attcgcggcc gcttctagag //