LOCUS BBa_K823026 8289 bp DNA linear 26-Dec-2024 DEFINITION pSBBs0K-Pspac (replicative Bacillus subtilis expression vector; IPTG inducible ACCESSION BBa_K823026 VERSION BBa_K823026.1 FEATURES Location/Qualifiers CDS 271..1353 /label=lacI CDS 2609..3469 /label=bla misc_feature 4093..5097 /label=repU CDS 5257..6027 /label=kan CDS 6250..6648 /label=ble CDS 6906..7871 /label=lacY promoter 8193..8258 /label=Pspac ORIGIN 1 tactagtagc ggccgctgca ggcatgcaag ctaattccgg tggaaacgag gtcatcattt 61 ccttccgaaa aaacggttgc atttaaatct tacatatgta atactttcaa agactacatt 121 tgtaagattt gatgtttgag tcggctgaaa gatcgtacgt accaattatt gtttcgtgat 181 tgttcaagcc ataacactgt agggatagtg gaaagagtgc ttcatctggt tacgatcaat 241 caaatattca aacggaggga gacgattttg atgaaaccag taacgttata cgatgtcgca 301 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt 361 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac 421 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt 481 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg 541 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg 601 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac 661 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc 721 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc 781 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt 841 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt 901 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg 961 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg 1021 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta 1081 gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac caccatcaaa 1141 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 1201 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 1261 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 1321 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagga 1381 tcctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 1441 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 1501 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 1561 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 1621 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 1681 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 1741 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 1801 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 1861 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 1921 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 1981 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 2041 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 2101 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 2161 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 2221 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 2281 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 2341 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 2401 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 2461 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 2521 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 2581 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 2641 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 2701 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 2761 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 2821 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 2881 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgccggc atcgtggtgt 2941 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 3001 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 3061 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 3121 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 3181 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 3241 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 3301 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 3361 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 3421 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 3481 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 3541 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 3601 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 3661 cctttcgtct tcaagagttc cttaaggaac gtacagacgg cttaaaagcc tttaaaaacg 3721 tttttaaggg gtttgtagac aaggtaaagg ataaaacagc acaattccaa gaaaaacacg 3781 atttagaacc taaaaagaac gaatttgaac taactcataa ccgagaggta aaaaaagaac 3841 gaagtcgaga tcagggaatg agtttataaa ataaaaaaag cacctgaaaa ggtgtctttt 3901 tttgatggtt ttgaacttgt tctttcttat cttgatacat atagaaataa cgtcattttt 3961 attttagttg ctgaaaggtg cgttgaagtg ttggtatgta tgtgttttaa agtattgaaa 4021 acccttaaaa ttggttgcac agaaaaaccc catctgttaa agttataagt gactaaacaa 4081 ataactaaat agatgggggt ttcttttaat attatgtgtc ctaatagtag catttattca 4141 gatgaaaaat caagggtttt agtggacaag acaaaaagtg gaaaagtgag accatggaga 4201 gaaaagaaaa tcgctaatgt tgattacttt gaacttctgc atattcttga atttaaaaag 4261 gctgaaagag taaaagattg tgctgaaata ttagagtata aacaaaatcg tgaaacaggc 4321 gaaagaaagt tgtatcgagt gtggttttgt aaatccaggc tttgtccaat gtgcaactgg 4381 aggagagcaa tgaaacatgg cattcagtca caaaaggttg ttgctgaagt tattaaacaa 4441 aagccaacag ttcgttggtt gtttctcaca ttaacagtta aaaatgttta tgatggcgaa 4501 gaattaaata agagtttgtc agatatggct caaggatttc gccgaatgat gcaatataaa 4561 aaaattaata aaaatcttgt tggttttatg cgtgcaacgg aagtgacaat aaataataaa 4621 gataattctt ataatcagca catgcatgta ttggtatgtg tggaaccaac ttattttaag 4681 aatacagaaa actacgtgaa tcaaaaacaa tggattcaat tttggaaaaa ggcaatgaaa 4741 ttagactatg atccaaatgt aaaagttcaa atgattcgac cgaaaaataa atataaatcg 4801 gatatacaat cggcaattga cgaaactgca aaatatcctg taaaggatac ggattttatg 4861 accgatgatg aagaaaagaa tttgaaacgt ttgtctgatt tggaggaagg tttacaccgt 4921 aaaaggttaa tctcctatgg tggtttgtta aaagaaatac ataaaaaatt aaaccttgat 4981 gacacagaag aaggcgattt gattcataca gatgatgacg aaaaagccga tgaagatgga 5041 ttttctatta ttgcaatgtg gaattgggaa cggaaaaatt attttattaa agagtagttc 5101 aacaaacggg ccagtttgtt gaagattaga tgctataatt gttattaaaa ggattgaagg 5161 atgcttagga agacgagtta ttaatagctg aataagaacg gtgctctcca aatattctta 5221 tttagaaaag caaatctaaa attatctgaa aagggaatga gaatagtgaa tggaccaata 5281 ataatgacta gagaagaaag aatgaagatt gttcatgaaa ttaaggaacg aatattggat 5341 aaatatgggg atgatgttaa ggctattggt gtttatggct ctcttggtcg tcagactgat 5401 gggccctatt cggatattga gatgatgtgt gtcatgtcaa cagaggaagc agagttcagc 5461 catgaatgga caaccggtga gtggaaggtg gaagtgaatt ttgatagcga agagattcta 5521 ctagattatg catctcaggt ggaatcagat tggccgctta cacatggtca atttttctct 5581 attttgccga tttatgattc aggtggatac ttagagaaag tgtatcaaac tgctaaatcg 5641 gtagaagccc aaacgttcca cgatgcgatt tgtgccctta tcgtagaaga gctgtttgaa 5701 tatgcaggca aatggcgtaa tattcgtgtg caaggaccga caacatttct accatccttg 5761 actgtacagg tagcaatggc aggtgccatg ttgattggtc tgcatcatcg catctgttat 5821 acgacgagcg cttcggtctt aactgaagca gttaagcaat cagatcttcc ttcaggttat 5881 gaccatctgt gccagttcgt aatgtctggt caactttccg actctgagaa acttctggaa 5941 tcgctagaga atttctggaa tgggattcag gagtggacag aacgacacgg atatatagtg 6001 gatgtgtcaa aacgcatacc attttgaacg atgacctcta ataattgtta atcatgttgg 6061 ttacgtattt attaacttct cctagtatta gtaattatca tggctgtcat ggcgcattaa 6121 cggaataaag ggtgtgctta aatcgggcca ttttgcgtaa taagaaaaag gattaattat 6181 gagcgaattg aattaataat aaggtaatag atttacatta gaaaatgaaa ggggatttta 6241 tgcgtgagaa tgttacagtc tatcccggca ttgccagtcg gggatattaa aaagagtata 6301 ggtttttatt gcgataaact aggtttcact ttggttcacc atgaagatgg attcgcagtt 6361 ctaatgtgta atgaggttcg gattcatcta tgggaggcaa gtgatgaagg ctggcgctct 6421 cgtagtaatg attcaccggt ttgtacaggt gcggagtcgt ttattgctgg tactgctagt 6481 tgccgcattg aagtagaggg aattgatgaa ttatatcaac atattaagcc tttgggcatt 6541 ttgcacccca atacatcatt aaaagatcag tggtgggatg aacgagactt tgcagtaatt 6601 gatcccgaca acaatttgat tagctttttt caacaaataa aaagctaaaa tctattatta 6661 atctgttcag caatcgggcg cgattgctga ataaaagata cgagagacct ctcttgtatc 6721 ttttttattt tgagtggttt tgtccgttac actagaaaac cgaaagacaa taaaaatttt 6781 attcttgctg agtctggctt tcggtaagct agacaaaacg gacaaaataa aaattggcaa 6841 gggtttaaag gtggagattt tttgagtgat cttctcaaaa aatactacct gtcccttgct 6901 gatttttaaa cgagcacgag agcaaaaccc ccctttgctg aggtggcgaa cgatgagcca 6961 ataatacgta cagacataat agtgccagcc agcagcaggg cgtttttccc accgatgcga 7021 ttaatgatca gtggcgcaaa gaacataatc gaggcgttaa gtaattcgcc cattgtcgtt 7081 acgtagccaa atacccgcgt accctgttca ccggtagcaa agaacgaagt aaagaaatta 7141 gcaaactgtt ggtcaaaaac atcgtaggtg caggaaacgc caataacata cagtgacaaa 7201 aaccacagtt ttggctgtct gaacagttcc agtgccagct taaggctaaa tgccgaatgg 7261 ttggcaccta ccgcattggc aaccgtggca gaagagggcg catccgtttt ggcgaaaaag 7321 agtaaaacgg cgaggatgag tgcacagcca gagcccagcc agaaaacaaa ctgattattg 7381 atggtgaaca tgatgccgac aatcgaggca cacagcgccc agccaacaca gccaaacatc 7441 cgcgcgcgac caaattcgaa attactgcga cggctgactt tctcaataaa tgcctctact 7501 gctggcgcac cggcgttaaa acaaaagcct agataaatac caccaacaat cgatcctact 7561 aaaatgttgt attgtaacag tggcccgaag ataaaaataa agaacggcgc aaacatcact 7621 aacatgccgg taataatcca cagcaggtat ttgcgcagcc cgagtttgtc agaaagcaga 7681 ccaaacagcg gttggaataa tagcgagaac agagaaatag cggcaaaaat aatacccgta 7741 tcacttttgc tgatatggtt gatgtcatgt agccaaatcg ggaaaaacgg gaagtaggct 7801 cccatgataa aaaagtaaaa gaaaaagaat aaaccgaaca tccaaaagtt tgtgtttttt 7861 aaatagtaca taatggattt ccttacgcga aatacgggca gacatggcct gcccggttat 7921 tattattttt gacaccagac caactggtaa tggtagcgac cggcgctcag ctggagttct 7981 acacagccca gtccagacta ttcggcactg aaattatggg tgaagtggtc aagacctcac 8041 taggcacctt aaaaatagcg caccctgaag aagatttatt tgaggtagcc cttgcctacc 8101 tagcttccaa gaaagatatc ctaacagcac aagagcggaa agatgttttg ttctacatcc 8161 agaacaacct ctgctaaaat tcctgaaaaa ttttgcaaaa agttgttgac tttatctaca 8221 aggtgtggca taatgtgtgg aattgtgagc ggataacaat taagcttgaa ttcgcggccg 8281 cttctagag //