LOCUS BBa_K823027 6193 bp DNA linear 22-Dec-2024 DEFINITION pSBBs2E: Empty backbone for integration into Bacillus subtilis lacA locus ACCESSION BBa_K823027 VERSION BBa_K823027.1 FEATURES Location/Qualifiers CDS 500..1300 /label=erm misc_feature 1600..2100 /label=lacA' CDS 4603..5400 /label=bla misc_feature 5600..6100 /label='lacA ORIGIN 1 gtcgcgatga ttaattaatt cagaacgctc ggttgccgcc gggcgttttt tatgcagcaa 61 tggcaagaac gtcccgggga gctcccgcgg gcggccgcac tcttcctttt tcaatattat 121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 241 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt 301 caagaattga tcctctagca caaaagaaaa acgaaatgat acaccaatca gtgcaaaaaa 361 agatataatg ggagataaga cggttcgtgt tcgtgctgac ttgcaccata tcataaaaat 421 cgaaacagca aagaatggcg gaaacgtaaa agaagttatg gaaataagac ttagaagcaa 481 acttaagagt gtgttgatag tgcagtatct taaaattttg tataatagga attgaagtta 541 aattagatgc taaaaatttg taattaagaa ggagtgatta catgaacaaa aatataaaat 601 attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa caattgaatt 661 taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta acgacgaaac 721 tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta ttcaacttat 781 cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat attctacagt 841 ttcaattccc taacaaacag aggtataaaa ttgttgggag tattccttac catttaagca 901 cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat ctgattgttg 961 aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg ctcttgcaca 1021 ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat cctaaaccaa 1081 aagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca gataaatatt 1141 ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt caactgttta 1201 ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta agtaccgtta 1261 cttatgagca agtattgtct atttttaata gttatctatt atttaacggg aggaaataat 1321 tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa tgtagataaa 1381 ttattaggta tactactgac agcttccaag gagctaaaga ggtccctaga ctctaggact 1441 ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1501 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc tctagctaag 1561 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactcttgt taactcctag 1621 agcggccgca tcgattcaca gtggcaatct cccccgtatt cgtttgaaat gtgccacatt 1681 aacagcgccg ggtgatgtcc gtatcgttct gctaataagc ggttgatgtg ccgtgttttt 1741 tctcggtaga ctttagatgt gaggcagtgg ttgtgccttc cgccgtgcag ctgtttgacg 1801 cgggaggcat tgacgcgcaa aacttccgga taggtttgcg acagccaggc cggacgggct 1861 ccgctcggcg ttgctaatat gacccggccg cctatactgt gaatccgctc aaaaatatca 1921 tccagccatt caaattgata tacgccctcc tccggctcaa gtgcgctcca tgcaaaaatg 1981 ccgacagaaa acgtattcgt atgagaaagc ttcatcagtt tgatatcgtc agctaaaata 2041 tcgggccgat ccagccactg atcggggttg tagtctcccc catggagcat aaattttgct 2101 tttgttacgt gcgttttttc aagctttgac atcaccccat cctgatattg tctgcatttg 2161 cgccggagca aaccaaaaac ctggtgagac acgccttgaa ttagtagaaa agaacttgaa 2221 gattttcaaa ggcatcgtta gtgaagtcat ggcgagcgga tttgacggca ttttcttagt 2281 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg 2341 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct 2401 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac 2461 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc 2521 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac 2581 ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat 2641 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg 2701 tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg 2761 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact 2821 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg 2881 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa 2941 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg 3001 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt 3061 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac 3121 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc 3181 attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa 3241 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac 3301 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg 3361 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 3421 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 3481 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 3541 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt 3601 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 3661 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 3721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 3781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 3841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 3901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 3961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 4021 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg 4081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 4141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 4201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 4261 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 4321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 4381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 4441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 4501 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 4561 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 4621 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 4681 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 4741 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 4801 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 4861 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc 4921 cggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 4981 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 5041 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 5101 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 5161 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac 5221 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 5281 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 5341 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 5401 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 5461 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 5521 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 5581 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 5641 gcgtatcacg aggccctttc gtcttcaaga atgatgggct aatgtgtgtt tacgacaatt 5701 ctcacttcat acttttccat cgtcaggtcg cctgacaata tgtctcctgt cattatgtcc 5761 ttcacactct gatcaaacgt gaccagctgt ttttcttccg tgaaattcat gacaaaaata 5821 taatcattgt cctgatcctg cctcgcttgt acggagacgc cttttccgtg ccgaaccgga 5881 aaaactggag agagagacag gtctgtgatc agaccctcat agaaatcacg ctgaaattga 5941 tcctccaaac gcgcgccgat aaaatacgcc ttgccctgct gatactcatg gcttgtgacc 6001 gctggcgtgc gcgcataaaa atcttcttga tacaccgctt ccactgaagc tgtctttaca 6061 tcaatcacgg ttgcataatc cttcatttca tatatttggc tgcggtagct gacagcgttt 6121 cgatccttcg gatacagggt gtccgtttca agaggctcaa ctccaaatat agcttgaaat 6181 cgatatctct gca //