BBa_K823040 1 BBa_K823040 Inverter: pBad-RyhB-pLac(R0011)-uof-lacZalpha 2012-09-09T11:00:00Z 2015-05-08T01:13:30Z E.coli, Plasmids This part reverses the positive Input of the pBad Promoter induced by Arabinose to a negative output of the lacZalpha Reporter, by the translational inhibitory action of the small RNA RyhB to uof translationally fused to lacZalpha. false false _1081_ 0 9472 9 It's complicated false Fusion of the small RNA directly to promotor to ensure exact +1 site and length. Translational fusion of uof to lacZalpha. These had to be done by fusionPCRs false Julia Bartels annotation2182369 1 uof range2182369 1 278 399 annotation2182368 1 pLac R0011 range2182368 1 213 267 annotation2182366 1 pBAD range2182366 1 1 123 annotation2182370 1 lacZalpha range2182370 1 400 704 annotation2182367 1 RyhB range2182367 1 124 202 BBa_K823040_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgttgtctcgcgatcaggaagaccctcgcggagaacctgaaagcacgacattgctcacattgcttccagtattacttagccagccgggtgctggcttttactagagctagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaagagctagaggtggttttcatttaggcgttacaattctatactgatacgcattatctcaagagcaaattctgtcacttcttctaatgaagtgaaccgcttagtaacaggacagattccgcatgactgataacaataccgccctaaagaaagctggcctgaaagtaacgcttcctcgtttaaaaatcctggaagttcttcaggagccggacaaccatcacgtcagtgcggatcccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z