LOCUS       BBa_K823049             1586 bp    DNA     linear       22-Dec-2024
DEFINITION  P<sub><i>cotYZ</i></sub> - <i>cotZ</i> (-2aa) - <i>gfp</i> - 
            Terminator
ACCESSION   BBa_K823049
VERSION     BBa_K823049.1  
FEATURES             Location/Qualifiers
     promoter        1..183
                     /label=PcotYZ
     promoter        150..157
                     /label=-10
     promoter        1..187
                     /label=BBa_K823030
     CDS             196..630
                     /label=cotZ
     CDS             196..630
                     /label=BBa_K823031
     CDS             639..1483
                     /label=gfpmut1
     misc_feature    639..1483
                     /label=BBa_K823039
     stem_loop       1499..1518
                     /label=T7 TE
     misc_feature    1525..1525
                     /label=stop
     polyA_site      1519..1532
                     /label=polya
     misc_feature    1492..1532
                     /label=BBa_B0012
     terminator      1492..1532
                     /label=BBa_B0012
     stem_loop       1553..1575
                     /label=stem_loop
     misc_feature    1541..1586
                     /label=BBa_B0011
     terminator      1541..1586
                     /label=BBa_B0011
     terminator      1492..1586
                     /label=BBa_B0014
ORIGIN
        1 gacagcaaca aatacactcg tagccatcct agttatcact cttgtcctct aggacctaaa
       61 agcagagcta aaaacgctct gctttttctt attttccaag catatgatga atatatagac
      121 gttcacccac accaagtggg gcacgggtac atatgttgtt aaggactaaa gtcaaatacc
      181 ctataattac tagagaaaac atcaagctgc gtgcgtgaag ctgtagaaaa tattgaagat
      241 ctgcaaaacg ctgttgaaga agactgcccg accggctgcc actctaagct tttatctgta
      301 agccattcgt taggcgacac agtgcctttt gcaatattta catcaaaatc aacgccatta
      361 gtcgccttcg gaaatgtcgg cgaactcgat aacggccctt gctttaatac agtatttttc
      421 agggtcgaaa gagtgcatgg aagctgtgca acactgtcat tattaatcgc atttgacgaa
      481 cacaaacaca ttttggactt caccgataaa gatacggtgt gtgaagtgtt ccgactcgaa
      541 aaaacgaact actgtattga agttgactta gactgcttct gcgcaatcaa ctgcttaaat
      601 cctcgattaa tcaatcgtac acatcatcat tactagaggg ttctgtagtc gacttctcac
      661 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
      721 tcattactgg atctatcaac aggagtccaa gctcagctaa ttaagcttat ttgtatagtt
      781 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc
      841 tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      901 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      961 ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt
     1021 ctgccatgat gtatacattg tgtgagttat agttgtattc caatttgtgt ccaagaatgt
     1081 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
     1141 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt
     1201 cctgtacata accttcgggc atggcactct tgaaaaagtc atgccgtttc atatgatctg
     1261 ggtatctcgc aaagcattga acaccataac cgaaagtagt gacaagtgtt ggccatggaa
     1321 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
     1381 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa
     1441 gaattgggac aactccagtg aaaagttctt ctcctttacg cattactaga gtcacactgg
     1501 ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga
     1561 ttattaatcc ggctttttta ttattt
//