BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_K823031 1 cotZ <i>cotZ</i>: <i>B. subtilis</i> spore crust protein (-2aa) 2012-09-05T11:00:00Z 2015-05-08T01:13:30Z B. subtillis W168 genome crust protein See all the details about the LMU-Munich 2012 project here: http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins false false _1081_ 0 12084 9 It's complicated false We designed it in Freiburg standard (Assembly 25) and used it for the display of fusion proteins on B. subtilis spores. false Franziska D??rr annotation2181858 1 cotZ range2181858 1 1 435 BBa_K823039 1 BBa_K823039 gfpmut1 (B. subtilis optimized + Freiburg standard) 2012-09-07T11:00:00Z 2015-05-08T01:13:30Z Plasmid pGFPamy This gfpmut3 gene was amplified from pGFPamy B. subtilis vector. It is constructed in the [[Help:Assembly_standard_25|Freiburg standard]] with RBS. prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT false false _1081_ 0 11555 9 It's complicated false We designed this part in the Freiburg standard for N- and C-terminal fusions. false Tamara Cikovic annotation2182335 1 gfpmut1 range2182335 1 1 845 BBa_K823030 1 PcotYZ P_<i>cotYZ</i>: <i>B. subtilis</i> promoter regulating expression of spore crust proteins 2012-09-05T11:00:00Z 2015-05-08T01:13:30Z B. subtilis W168 genome PcotYZ: B. subtilis promoter regulates spore crust proteins and is activated in late sporulation. PcotYZ regulates the crust protein cotZ and cotY. false false _1081_ 0 12084 9 It's complicated false We use this promoter for the expression of fusion proteins on B. subtilis spores. false Franziska D??rr annotation2200628 1 -10 range2200628 1 150 157 annotation2182004 1 PcotYZ range2182004 1 1 183 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939311 1 BBa_B0011 component939303 1 BBa_B0012 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_K823049 1 GFP-spore P_<i>cotYZ</i> - <i>cotZ</i> (-2aa) - <i>gfp</i> - Terminator 2012-09-09T11:00:00Z 2015-05-08T01:13:30Z genomic B. subtilis, registry (Termiantor), Plasmid (pGFPamy) This composite part expresses a gfp fusion protein on a B. subtilis spore. false false _1081_ 0 11555 9 It's complicated true This part is cloned in the B. subtilis pSBBs1C backbone vector for direct integration in the amyE locus of B. subtilis. false Franziska D??rr component2182403 1 BBa_K823031 component2182412 1 BBa_B0014 component2182401 1 BBa_K823030 component2182405 1 BBa_K823039 annotation2182412 1 BBa_B0014 range2182412 1 1492 1586 annotation2182403 1 BBa_K823031 range2182403 1 196 630 annotation2182405 1 BBa_K823039 range2182405 1 639 1483 annotation2182401 1 BBa_K823030 range2182401 1 1 187 BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K823031_sequence 1 aaaacatcaagctgcgtgcgtgaagctgtagaaaatattgaagatctgcaaaacgctgttgaagaagactgcccgaccggctgccactctaagcttttatctgtaagccattcgttaggcgacacagtgccttttgcaatatttacatcaaaatcaacgccattagtcgccttcggaaatgtcggcgaactcgataacggcccttgctttaatacagtatttttcagggtcgaaagagtgcatggaagctgtgcaacactgtcattattaatcgcatttgacgaacacaaacacattttggacttcaccgataaagatacggtgtgtgaagtgttccgactcgaaaaaacgaactactgtattgaagttgacttagactgcttctgcgcaatcaactgcttaaatcctcgattaatcaatcgtacacatcatcat BBa_K823039_sequence 1 ggttctgtagtcgacttctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagctcagctaattaagcttatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtggtctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgccgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcat BBa_K823030_sequence 1 gacagcaacaaatacactcgtagccatcctagttatcactcttgtcctctaggacctaaaagcagagctaaaaacgctctgctttttcttattttccaagcatatgatgaatatatagacgttcacccacaccaagtggggcacgggtacatatgttgttaaggactaaagtcaaataccctataat BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K823049_sequence 1 gacagcaacaaatacactcgtagccatcctagttatcactcttgtcctctaggacctaaaagcagagctaaaaacgctctgctttttcttattttccaagcatatgatgaatatatagacgttcacccacaccaagtggggcacgggtacatatgttgttaaggactaaagtcaaataccctataattactagagaaaacatcaagctgcgtgcgtgaagctgtagaaaatattgaagatctgcaaaacgctgttgaagaagactgcccgaccggctgccactctaagcttttatctgtaagccattcgttaggcgacacagtgccttttgcaatatttacatcaaaatcaacgccattagtcgccttcggaaatgtcggcgaactcgataacggcccttgctttaatacagtatttttcagggtcgaaagagtgcatggaagctgtgcaacactgtcattattaatcgcatttgacgaacacaaacacattttggacttcaccgataaagatacggtgtgtgaagtgttccgactcgaaaaaacgaactactgtattgaagttgacttagactgcttctgcgcaatcaactgcttaaatcctcgattaatcaatcgtacacatcatcattactagagggttctgtagtcgacttctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagctcagctaattaagcttatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtggtctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgccgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcattactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z