LOCUS       BBa_K823050             1756 bp    DNA     linear       22-Dec-2024
DEFINITION  luc luciferase, codon optimized for Bacillus subtilis+ RBS+Term
ACCESSION   BBa_K823050
VERSION     BBa_K823050.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1653
                     /label=BBa_K823028
     stem_loop       1669..1688
                     /label=T7 TE
     misc_feature    1695..1695
                     /label=stop
     polyA_site      1689..1702
                     /label=polya
     misc_feature    1662..1702
                     /label=BBa_B0012
     terminator      1662..1702
                     /label=BBa_B0012
     stem_loop       1723..1745
                     /label=stem_loop
     misc_feature    1711..1756
                     /label=BBa_B0011
     terminator      1711..1756
                     /label=BBa_B0011
     terminator      1662..1756
                     /label=BBa_B0014
ORIGIN
        1 atggaagatg ctaaaaacat caaaaaaggc cctgctcctt tctaccctct tgaagatggc
       61 acagctggcg aacaacttca taaagctatg aaacgttacg ctcttgttcc tggcacaatc
      121 gctttcacag atgctcatat cgaagttgat atcacatacg ctgaatactt cgaaatgtct
      181 gttcgtcttg ctgaagctat gaaacgttac ggccttaaca caaaccatcg tatagttgta
      241 tgcagcgaaa actctcttca attcttcatg cctgttcttg gcgctctttt catcggcgtt
      301 gctgttgctc ctgctaacga tatctacaac gaacgtgaac ttcttaactc tatgggcatc
      361 tctcagccta ctgttgtctt cgtatctaaa aaaggccttc aaaaaatcct taacgttcaa
      421 aaaaaacttc ctatcatcca aaaaatcatc atcatggatt ctaaaacaga ttaccaaggc
      481 ttccaatcta tgtacacatt cgttacatct catcttcctc ctggcttcaa cgaatacgat
      541 ttcgttcctg aatctttcga tcgtgataaa acaatcgctc ttatcatgaa ctcttctggc
      601 tctacaggcc ttcctaaagg cgttgctctt cctcatcgta cagcttgcgt tcgtttctct
      661 catgctcgtg atcctatctt cggcaaccaa atcatccctg acactgctat cctcagcgtt
      721 gttcctttcc atcatggctt cggcatgttc acaacacttg gctaccttat ctgcggcttc
      781 cgtgttgttc ttatgtaccg tttcgaagaa gaacttttcc ttcgttctct tcaagattac
      841 aaaatccaat ctgctcttct tgttcctaca cttttctctt tcttcgctaa atctacactt
      901 atcgataaat acgatctttc taaccttcat gaaatcgctt ctgggggcgc acctctcagc
      961 aaagaagttg gcgaagctgt tgctaaacgt ttccatcttc ctggcatccg tcaaggctac
     1021 ggccttacag aaacaacatc tgctatcctt atcacacctg aaggcgatga taaacctggc
     1081 gctgttggca aagttgttcc tttcttcgaa gctaaagttg ttgatcttga tacaggcaaa
     1141 acacttggcg ttaaccaacg tggcgaactt tgcgttcgtg gccctatgat catgtctggc
     1201 tacgttaaca accctgaagc tacaaacgct cttatcgata aagatggctg gcttcattct
     1261 ggcgatatcg cttactggga tgaagatgaa catttcttca tcgttgatcg tcttaaatct
     1321 cttatcaaat acaaaggcta ccaagttgct cctgctgaac ttgaatctat ccttcttcaa
     1381 catcctaaca tcttcgatgc tggcgttgct ggccttcctg atgatgatgc tggcgaactt
     1441 cctgctgctg ttgttgttct tgaacatggc aaaacaatga cagaaaaaga aatcgttgat
     1501 tacgttgctt ctcaagttac aacagctaaa aaacttcgtg gcggcgttgt tttcgttgat
     1561 gaagttccta aaggccttac aggcaaactt gatgctcgta aaatccgtga aatccttatc
     1621 aaagctaaaa aaggcggcaa gatagctgtc taatactaga gtcacactgg ctcaccttcg
     1681 ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc
     1741 ggctttttta ttattt
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