##gff-version 3
##sequence-region BBa_K823053 1 3196
BBa_K823053	.	terminator	3102	3196	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K823053	.	terminator	3151	3196	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K823053	.	stem_loop	3163	3185	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K823053	.	engineered_region	3151	3196	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K823053	.	terminator	3102	3142	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K823053	.	polyA_site	3129	3142	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K823053	.	engineered_region	3102	3142	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K823053	.	stem_loop	3109	3128	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K823053	.	stop_codon	3135	3135	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K823053	.	engineered_region	1	3093	.	+	0	ID=BBa_I732017;Name=BBa_I732017
BBa_K823053	.	CDS	19	3093	.	+	0	ID=BBa_I732005;Name=BBa_I732005;Parent=BBa_I732017
BBa_K823053	.	stop_codon	3091	3093	.	+	0	ID=annotation1936872;Name=STOP;Parent=BBa_I732005
BBa_K823053	.	point_mutation	3042	3042	.	+	0	ID=annotation1936869;Name=C->T;Parent=BBa_I732005
BBa_K823053	.	start_codon	19	21	.	+	0	ID=annotation1936871;Name=START;Parent=BBa_I732005
BBa_K823053	.	CDS	19	3093	.	+	0	ID=annotation1936870;Name=lacZ;Parent=BBa_I732005
BBa_K823053	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732017
BBa_K823053	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K823053
aaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctg
gcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcg
cccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagc
tggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgccca
tctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgct
cacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcat
ctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttac
gcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcg
gatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgc
tttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaa
cagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtgg
tggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctat
cgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgc
ggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctct
gcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgc
tgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaata
ttgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaac
gcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaat
cacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacacca
cggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccat
caaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtctt
ggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatc
agtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcg
ccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcag
tttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcc
tgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaaca
gttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaac
gcgaccgcatggtcagaagccgggcacatcagcgcctggcagcagtggcgtctggcggaaaacctcagtgtgacgctcc
ccgccgcgtcccacgccatcccgcatctgaccaccagcgaaatggatttttgcatcgagctgggtaataagcgttggca
atttaaccgccagtcaggctttctttcacagatgtggattggcgataaaaaacaactgctgacgccgctgcgcgatcag
ttcacccgtgcaccgctggataacgacattggcgtaagtgaagcgacccgcattgaccctaacgcctgggtcgaacgct
ggaaggcggcgggccattaccaggccgaagcagcgttgttgcagtgcacggcagatacacttgctgatgcggtgctgat
tacgaccgctcacgcgtggcagcatcaggggaaaaccttatttatcagccggaaaacctaccggattgatggtagtggt
caaatggcgattaccgttgatgttgaagtggcgagcgatacaccgcatccggcgcggattggcctgaactgccagctgg
cgcaggtagcagagcgggtaaactggctcggattagggccgcaagaaaactatcccgaccgccttactgccgcctgttt
tgaccgctgggatctgccattgtcagacatgtataccccgtacgtcttcccgagcgaaaacggtctgcgctgcgggacg
cgcgaattgaattatggcccacaccagtggcgcggcgacttccagttcaacatcagccgctacagtcaacagcaactga
tggaaaccagccatcgccatctgctgcacgcggaagaaggcacatggctgaatatcgacggtttccatatggggattgg
tggcgacgactcctggagcccgtcagtatcggcggaatttcagctgagcgccggtcgctaccattaccagttggtctgg
tgtcaaaaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatata
aaaagccagattattaatccggcttttttattattt