LOCUS BBa_K823054 6313 bp DNA linear 22-Dec-2024 DEFINITION Sporovector ACCESSION BBa_K823054 VERSION BBa_K823054.1 FEATURES Location/Qualifiers CDS 1..247 /label=hom CDS 247..756 /label=thrC misc_feature 766..772 /label=EcoRI promoter 773..958 /label=PcotYZ misc_feature 959..964 /label=XbaI CDS 1192..1872 /label=RFP misc_feature 2035..2040 /label=NgoMIV CDS 2038..2439 /label=cgeA misc_feature 2431..2436 /label=AgeI stem_loop 2448..2542 /label=B0014 misc_feature 2543..2548 /label=PstI CDS 2738..3412 /label=spec CDS 3555..3983 /label=thrC CDS 3980..4390 /label=thrB CDS 5243..6103 /label=bla CDS 6258..6313 /label=hom ORIGIN 1 aattcatgta aaagatgagg ttggttcatt ctcgaaaatt acatctgtgt tctcagagcg 61 gggcgtgacg tttgaaaaaa tccttcagct gccaattaaa ggccatgatg agttagctga 121 aatcgtaatt gtcacacatc atacatcaga agctgatttc agtgatatcc tgcaaaacct 181 aaatgatttg gaagtcgttc aagaagtcaa aagcacatat cgtgtagaag ggaacggttg 241 gagctaatgt ggaaaggact tatccatcaa tataaagaat ttttacctgt aacagatcaa 301 acaccggcgc taactttaca tgaaggaaac acacctctta ttcacctgcc gaagctgtct 361 gagcagctcg gaattgagct tcatgtcaaa acggaaggcg tcaatcctac gggatcattt 421 aaagatcgcg gaatggttat ggctgtggca aaggcaaaag aagaaggcaa tgacacgatt 481 atgtgcgcgt caacaggtaa cacttccgct gctgcggcag catatgcagc ccgtgctaac 541 atgaaatgca ttgtcatcat cccgaacgga aaaattgcat ttggaaaact cgctcaagct 601 gtcatgtacg gagccgagat tatcgcaatt gacggaaact ttgacgatgc gcttaaaatt 661 gtccgttcca tctgtgagaa atcaccgatt gcccttgtca actcagtcaa cccttaccgc 721 attgaaggcc aaaaaactgc tgccttcgga tcctagaagc ttatcgaatt cgacagcaac 781 aaatacactc gtagccatcc tagttatcac tcttgtcctc taggacctaa aagcagagct 841 aaaaacgctc tgctttttct tattttccaa gcatatgatg aatatataga cgttcaccca 901 caccaagtgg ggcacgggta catatgttgt taaggactaa agtcaaatac cctataattc 961 tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 1021 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 1081 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 1141 tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta gatggcttcc 1201 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac 1261 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc 1321 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg 1381 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg 1441 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt 1501 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa 1561 ctgcgtggta ccaacttccc gtccgatggt ccggttatgc agaaaaaaac catgggttgg 1621 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg 1681 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct 1741 aaaaaacctg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc 1801 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactcctcc 1861 ggagcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa 1921 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 1981 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatagccggc 2041 gaaaatgcac agttaaaaaa ggatttaatt aaagccgttt taagcccttt atttccaact 2101 gcaacagaag gaggagaaaa tatggatagt aatcttaaag ccttgcttga tgctgccatc 2161 gatcaaaaag tagatgaaag tgaaacggtt acggcagaat ctattttaga cccttctctt 2221 ccggcaagat ggatttttgc cagaattacg ccaggcacta caatcagcat tgtgactgat 2281 tcaggtgaca tgatcggacc agttgttttc gttgctttcg atcaggttca cgggatcgta 2341 tttgtaacac aggaaagctc cgtcactccg gcaggccaag ctacaacatt aattgatgta 2401 gacaaagtag aaagcgttac gttcttttca accggttaat actagagtca cactggctca 2461 ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa gccagattat 2521 taatccggct tttttattat ttctgcagga taaaaaattt agaagccaat gaaatctata 2581 aataaactaa attaagttta tttaattaac aactatggat ataaaatagg tactaatcaa 2641 aatagtgagg aggatatatt tgaatacata cgaacaaatt aataaagtga aaaaaatact 2701 tcggaaacat ttaaaaaata accttattgg tacttacatg tttggatcag gagttgagag 2761 tggactaaaa ccaaatagtg atcttgactt tttagtcgtc gtatctgaac cattgacaga 2821 tcaaagtaaa gaaatactta tacaaaaaat tagacctatt tcaaaaaaaa taggagataa 2881 aagcaactta cgatatattg aattaacaat tattattcag caagaaatgg taccgtggaa 2941 tcatcctccc aaacaagaat ttatttatgg agaatggtta caagagcttt atgaacaagg 3001 atacattcct cagaaggaat taaattcaga tttaaccata atgctttacc aagcaaaacg 3061 aaaaaataaa agaatatacg gaaattatga cttagaggaa ttactacctg atattccatt 3121 ttctgatgtg agaagagcca ttatggattc gtcagaggaa ttaatagata attatcagga 3181 tgatgaaacc aactctatat taactttatg ccgtatgatt ttaactatgg acacgggtaa 3241 aatcatacca aaagatattg cgggaaatgc agtggctgaa tcttctccat tagaacatag 3301 ggagagaatt ttgttagcag ttcgtagtta tcttggagag aatattgaat ggactaatga 3361 aaatgtaaat ttaactataa actatttaaa taacagatta aaaaaattat aaaaaaattg 3421 aaaaaatggt ggaaacactt ttttcaattt ttttgtttta ttatttaata tttgggaaat 3481 attcattcta attggtaatc agattttaga aaacaataaa cccttgcata gggggatctc 3541 gaggccttcc gaaaatgcgc ggctttgaag ctgagggtgc ggcggcaatc gtgcgcaatg 3601 aagtgattga aaatccggaa acaatagcga cagccattcg tatcggaaac ccggcaagct 3661 gggacaaagc tgtaaaggca gccgaggaat ccaatgggaa aattgacgaa gtcactgatg 3721 atgaaatcct tcacgcatat cagctgatcg cccgtgtaga aggcgtgttt gcagaaccag 3781 gttcttgcgc gtctatcgca ggagtgctga aacaggtgaa atccggagaa attccgaaag 3841 gcagcaaggt cgtagctgtg ttaacaggaa acggactgaa agatccgaac acagcggtcg 3901 acatttcaga aatcaagcct gtcacattgc cgactgatga agacagcatc cttgaatatg 3961 taaaaggagc ggcccgtgta tgaacgaagc cgacatgctg ttctctgtca ctgttcccgg 4021 aagcacagct aacctaggcc ccggctttga ttcagtcgga atggcgctca gcagatattt 4081 gaagctgacc gtctttgaaa gcgacaaatg gtcttttgag gctgaaacag aaacagtcgc 4141 cggaattaat tcgagctcct ttaacttcct caccagtagt tgtatcggta ccataagtag 4201 aagcagcaac ccaagtagct ttaccagcat ccggttcaac cagcatagta agaatcttac 4261 tggacatcgg cagttcttcg aacagtgcgc caactaccag ctctttctac agttcattca 4321 gggcaccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc 4381 tgtctcttga tccatggatt acgcgcgacc ttcagcatca ccggcatgtc cccctggcgg 4441 acgggaagta tccagctcga ggtcgggccg cgttgctggc gtttttccat aggctccgcc 4501 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 4561 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 4621 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 4681 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 4741 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 4801 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 4861 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 4921 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 4981 atagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 5041 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 5101 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 5161 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 5221 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 5281 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 5341 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 5401 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 5461 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 5521 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc tggcatcgtg gtgtcacgct 5581 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 5641 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 5701 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 5761 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 5821 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac 5881 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 5941 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 6001 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 6061 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 6121 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 6181 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 6241 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 6301 gtcttcaaga att //