LOCUS       BBa_K824009             1381 bp    DNA     linear       09-May-2024
DEFINITION  Arsenic Promoter with GFP Reporter (Arsenic Detector)
ACCESSION   BBa_K824009
VERSION     BBa_K824009.1  
FEATURES             Location/Qualifiers
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    1..518
                     /label=BBa_J33201
     CDS             525..1244
                     /label=GFP protein
     CDS             525..1244
                     /label=BBa_E0040
     stem_loop       1264..1307
                     /label=stem_loop
     misc_feature    1253..1332
                     /label=BBa_B0010
     terminator      1253..1332
                     /label=BBa_B0010
     stem_loop       1348..1367
                     /label=T7 TE
     misc_feature    1374..1374
                     /label=stop
     polyA_site      1368..1381
                     /label=polya
     misc_feature    1341..1381
                     /label=BBa_B0012
     terminator      1341..1381
                     /label=BBa_B0012
     misc_feature    525..1381
                     /label=BBa_I13401
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
      481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagatgcgt aaaggagaag
      541 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      601 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      661 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
      721 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
      781 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      841 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      901 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      961 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
     1021 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     1081 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     1141 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     1201 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat
     1261 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1321 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1381 a
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