BBa_K826000 1 BBa_K826000 cytochrome which catalyzes the terminal oxidation of alkanes 2012-09-24T11:00:00Z 2015-05-08T01:13:30Z Alcanivorax borkumensis SK2 genome it is a standard biobrick part, which encode one type of cytochrome involved in hydrocarbon catabolism, espicially for the medium-chain-length hydrocarbone acording to our study. branched chain or aromatic hydrocarbon need to be study study for further. false false _1084_ 0 12311 9 It's complicated false in the fusion for with FDR and FDX, it located between these two gene, the same as in genome, only this, it can have the highest potential for its function. It has two enzyme point in the sequence (if you want to get it from bacterie), so site mutation is needed. the primer we design are as follows. P450 EcoRI (site 887) 5??? GTCCTTTGGAGTTCATTGGTAATCTCGC 3??? 68.7 46.4 5??? TACCAATGAACTCCAAAGGACGATTGAT 3??? 67.5 39.3 PstI (site 1281) 5??? TTGCCGAACTTCAGCTGCGTATTCTGTG 3??? 71.5 50.0 5??? TACGCAGCTGAAGTTCGGCAAGGCGGTT 3??? 76.7 57.1 false Kaige Zhang BBa_K826000_sequence 1 atgtcaacgagttcaagtacaagtaatgacatccaggcaaaaataattaacgccacatccaaagtcgtgccaatgcatctacagatcaaggcactaaaaaacttgatgaaggtgaagcggaagaccattggcacttcccgccctcaggtgcactttgttgaaaccgatttgcctgacgtcaatgatttggcgatagaagatatcgatacgagtaacccttttttataccgacaaggtaaggcgaatgcgtactttaagcggttgcgtgatgaagcgccggtgcactatcagaagaacagtgctttcgggccgttctggtcggtaacacgctacgaagatatcgtcttcgtggacaagagccatgatttgttttccgccgaaccccaaattatcttgggtgatcctccggaaggcctgtcggttgaaatgttcatcgctatggatcctcccaagcacgacgtacagcgtcgggcagtccagggtgttgttgcgcccaagaacctgaaagaaatggaaggactgatccgcaagcgcaccggggacgtactggatagcctgccgttggacactccgttcaactgggtgccggtggtgtcgaaagagctgaccgggcgcatgctagcctcactgttagatttcccgtatgacgaacgcgaaaaactggttggctggtcggatcgattgtccggcgcgtcctcggcaaccggcggcgagtttacgaatgaagatgtgttttttgatgacgcggcagatatggcgtgggctttctccaagctttggcgtgataaagaagcccgtcaaaaagcaggtgaagagccgggtttcgatttgatcagcatgcttcagtccaatgaagacacaaaagatctgatcaatcgtcctttggagttcattggtaatctcgcgttgttgattgttggcggtaatgacaccacgcgtaactcaatgagcgggggggtgctggctttaaatcagttcccagagcaattcgagaagctaaaggcgaacccaaagcttatccccaatatggtctctgaaatcattcgctggcaaacgccgcttgcgtatatgcgccgggttgccaagcaggatgtggagctgaacggacagaccatcaagaagggtgatcgcgtgctgatgtggtatgcgtcgggcaaccaggatgagagaaaatttgagaatcctgagcaattcatcatcgaccgcaaagatacgcgtaaccatgtgtcgtttggttatggggttcaccgttgtatgggcaaccgccttgccgaacttcagctgcgtattctgtgggaagagcttctccctcgctttgaaaacatcgaagtgatcggtgagccggagcgcgtgcaatcgaactttgtgcggggctattccaagatgatggttaagttgacggctaaaaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z