BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_K1109005
1
BBa_K1109005
Anti-legionella unit
2013-09-22T11:00:00Z
2015-05-08T01:09:10Z
Oligonucleotide sequence of the anti-legionella peptide was obtained from genomic sequence, and sequence of the PelB leader peptide was obtained from iGEM database.
Some Staphylococcus strains produce anti-Legionella peptide. ORF for anti-legionella peptide specifically encodes for a 22 amino acid peptide. Anti-legionella unit is a biobrick for suitable suffix and prefix regions. It contains a PelB leader sequence in the upstream of the anti-legionella peptide ORF to direct the peptide outside the cell.
false
false
_1420_
0
11226
9
It's complicated
false
Anti-legionella unit is a biobrick for suitable suffix and prefix regions. It was derived from synthetic oligonucleotides by tandem hybridization reactions, and amplified by PCR.
false
Dr. ??zlem Darcansoy İşeri
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939303
1
BBa_B0012
range939303
1
1
41
annotation939311
1
BBa_B0011
range939311
1
50
95
BBa_K1109001
1
BBa_K1109001
LqsR promoter
2013-09-22T11:00:00Z
2015-05-08T01:09:10Z
Legionella pneumophila genomic lqs gene cluster was cloned into pNT-1 plasmid(Cellular Microbiology 9(12):2903-2920, 2007.) LqsR promoter region was amplified by PCR as a biobrick.
LqsR is the gene encoding response regulator component of the legionella quorum sensing (lqs). LqsR promoter region is controlled by its own response regulator LqsR.
false
false
_1420_
0
11226
9
Not in stock
false
This part is a new biobrick with prefix and suffix regeions.
false
Dr. ??zlem Darcansoy İşeri
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1109003
1
BBa_K1109003
LqsR promoter with RBS
2013-09-22T11:00:00Z
2015-05-08T01:09:10Z
Legionella pneumophila genomic lqs gene cluster was cloned into pNT-1 plasmid (Cellular Microbiology 9(12):2903-2920, 2007.) LqsR promoter region was amplified by PCR as a biobrick, and ligated with RBS (BBa_B0034).
LqsR is the gene encoding response regulator component of the legionella quorum sensing (lqs). LqsR promoter region is controlled by its own response regulator LqsR. This composite part contains RBS downstream of the promoter region. When any ORF is cloned to the downstream of this composite part, it can be expressed in response to legionella auto-inducer (LAI-I).
false
false
_1420_
0
11226
9
It's complicated
false
LqsR promoter part is a new biobrick with prefix and suffix regions, and RBS (BBa_B0034) was ligated to downstream of it.
false
Dr. ??zlem Darcansoy İşeri
component2358476
1
BBa_B0034
component2358474
1
BBa_K1109001
annotation2358474
1
BBa_K1109001
range2358474
1
1
266
annotation2358476
1
BBa_B0034
range2358476
1
275
286
BBa_K830000
1
BBa_K830000
anti legionella cassette
2013-10-01T11:00:00Z
2015-05-08T01:13:32Z
Anti-legionella unit synthesized. Other part of this composite is LqsR promoter. And LqsR promoter region was amplified by PCR as a biobrick, and ligated with RBS (BBa_B0034).
anti-legionella (Warnericin RK) peptide, which is directed to periplasmic space by pelB leader sequence, and some proteins are transcribed with lqsR promoter.lqsR promoter is activated by phosphorylated lqsR. This provides synthesis of anti-legionella (Warnericin RK) peptide and marker protein in case of lqsA presence in environment.
false
false
_1420_
0
11224
9
Not in stock
false
None
false
Berkhan GEN??
component2367102
1
BBa_B0014
component2367094
1
BBa_K1109003
component2367095
1
BBa_K1109005
annotation2367094
1
BBa_K1109003
range2367094
1
1
286
annotation2367102
1
BBa_B0014
range2367102
1
436
530
annotation2367095
1
BBa_K1109005
range2367095
1
293
427
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K830000_sequence
1
gagcaaaacgttccaaagttatatcctttctatgaacttcaaataacctgtggctccatcatcagaacctggctaacttgtgaataaagtaacatgattgtttattataaccaaattatttaggttttatattcaccgggctttttggagtaagtattcaaaataagcgctcttaaaatcaaagctcaaaattagacaagttattgaatataagtctatattattaaataagtaccttatcttagctaagaaatcaaaaaaggatatactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatgcaatttatcacagatcttatcaaaaaagcagtagatttcttcaaaggtttatttggtaacaaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K1109001_sequence
1
gagcaaaacgttccaaagttatatcctttctatgaacttcaaataacctgtggctccatcatcagaacctggctaacttgtgaataaagtaacatgattgtttattataaccaaattatttaggttttatattcaccgggctttttggagtaagtattcaaaataagcgctcttaaaatcaaagctcaaaattagacaagttattgaatataagtctatattattaaataagtaccttatcttagctaagaaatcaaaaaaggata
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_K1109005_sequence
1
atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatgcaatttatcacagatcttatcaaaaaagcagtagatttcttcaaaggtttatttggtaacaaataa
BBa_K1109003_sequence
1
gagcaaaacgttccaaagttatatcctttctatgaacttcaaataacctgtggctccatcatcagaacctggctaacttgtgaataaagtaacatgattgtttattataaccaaattatttaggttttatattcaccgggctttttggagtaagtattcaaaataagcgctcttaaaatcaaagctcaaaattagacaagttattgaatataagtctatattattaaataagtaccttatcttagctaagaaatcaaaaaaggatatactagagaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z