BBa_K831001 1 BBa_K831001 HipB antitoxin from Escherichia coli K12 2012-09-25T11:00:00Z 2015-05-08T01:13:32Z This part was extracted of the genome of Escherichia coli K12 MG1655 using high fidelity PCR HipB is the antitoxin of the HipAB toxin-antitoxin (TA) module. HipB neutralizes the toxin effect of HipA due to protein-protein interactions. false false _1090_ 0 9809 9 It's complicated true No false Silvia J Ca??as annotation2199138 1 HipB range2199138 1 1 267 BBa_K831009 1 BBa_K831009 Inducible HipB antitoxin under the control of lac promoter 2012-09-25T11:00:00Z 2015-05-08T01:13:32Z This part was extracted of the genome of Escherichia coli K12 MG1655 using high fidelity PCR HipB is the antitoxin of the HipAB toxin-antitoxin (TA) module. HipB neutralizes the toxin effect of HipA due to protein-protein interactions. false false _1090_ 0 9809 9 It's complicated true None false Silvia J Ca??as component2199416 1 BBa_R0010 component2199424 1 BBa_K831001 annotation2199424 1 BBa_K831001 range2199424 1 207 473 annotation2199416 1 BBa_R0010 range2199416 1 1 200 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961227 1 start range1961227 1 173 173 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K831001_sequence 1 atgatgagctttcagaagatctatagcccaacgcaattggcgaatgcaatgaaactggttcgccagcaaaatggctggacgcagagcgagctggcgaaaaaaattggtattaagcaggcgacgatttccaatttcgaaaacaaccctgacaataccacgctcacgacattttttaagattttacagtcgcttgaactctcaatgacgctatgcgacgcgaaaaatgcctcgccagaatcaacagaacagcaaaatctggagtggtaa BBa_K831009_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagatgatgagctttcagaagatctatagcccaacgcaattggcgaatgcaatgaaactggttcgccagcaaaatggctggacgcagagcgagctggcgaaaaaaattggtattaagcaggcgacgatttccaatttcgaaaacaaccctgacaataccacgctcacgacattttttaagattttacagtcgcttgaactctcaatgacgctatgcgacgcgaaaaatgcctcgccagaatcaacagaacagcaaaatctggagtggtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z