LOCUS BBa_K831015 1411 bp DNA linear 23-Dec-2024 DEFINITION Inducible HipA7 toxin under the control of prm promoter ACCESSION BBa_K831015 VERSION BBa_K831015.1 FEATURES Location/Qualifiers misc_feature 9..25 /label=OR1 misc_feature 33..49 /label=OR2 promoter 48..53 /label=-35 misc_feature 59..65 /label=mutate to obliterate OR3 promoter 71..76 /label=-10 promoter 1..82 /label=BBa_I12007 CDS 89..1411 /label=HipA7 CDS 89..1411 /label=BBa_K831000 ORIGIN 1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa 61 atagtggtga tagatttaac gttactagat gcctaaactt gtcacttgga tgaacaacca 121 gcgggtaggc gagttaacga agttagccaa cggcgcgcac acctttaagt atgcaccgga 181 gtggttagca agccgttatg ccagaccgtt gtcactttcg ctgccattgc agagggggaa 241 tatcacctct gatgccgtat ttaacttctt cgataacctg ttacccgata gcccgattgt 301 acgtgaccgg atcgttaaac gttatcatgc caaatccaga caaccgtttg atttattgtc 361 agaaataggg cgagacagcg ttggtgccgt gacgttaata cccgaagacg aaaccgtaac 421 gcatccgata atggcatggg aaaagcttac tgaagccaga cttgaagaag tattaacggc 481 ttataaagca gatatcccgc taggcatgat tagagaagaa aatgactttc gcatctcggt 541 tgctggcgca caggagaaga cagcactgct cagaataggc aatgactggt gcattccgaa 601 aggaataacg ccgacgacgc acatcattaa attaccgatt ggcgaaatca ggcagcccaa 661 tgcgacgctc gatctcagcc aaagcgttga taatgagtat tactgtctgc tgctggcgaa 721 agaacttggg ttgaatgttc cggacgcaga aatcattaaa gcgggaaatg tgcgcgcgtt 781 agcggtcgaa cgttttgaca ggcgttggaa tgctgagcga acggttttac ttcgcttgcc 841 acaggaggat atgtgtcaga cattcggttt accttcatcg gtgaaatatg aatcagatgg 901 aggcccaggc atcgcgcgga tcatggcttt tttgatgggg tccagcgagg cgctgaaaga 961 tcgctatgat tttatgaaat tccaggtctt ccagtggttg attggcgcaa cggacggtca 1021 tgcaaaaaac ttctccgtat ttattcaggc tggcggcagt tatcgactca cgccatttta 1081 cgacatcatt tcagcatttc cggtccttgg cggtacggga atacacatca gcgatctcaa 1141 actggcaatg gggcttaacg catccaaagg caaaaaaacg gcaatcgata aaatttatcc 1201 gcgacatttt ttggcgacag caaaggtgct gagattcccg gaagtgcaga tgcatgaaat 1261 cctgagtgac tttgccagaa tgattccagc agcactggat aacgtgaaga cttcattacc 1321 gacagatttt ccggagaacg tggtgacggc agttgaaagc aatgtgttga ggttgcatgg 1381 acggttaagc cgagaatacg gtagtaagtg a //